A Novel β-Glucosidase From Chryseobacterium scophthalmum 1433 for Efficient Rubusoside Production From Stevioside

Yan, Zhenxin; Cao, Xueting; Yang, Xiao; Yang, Shida; Xu, Lixin; Jiang, Xukai; Xiao, Min

doi:10.3389/fmicb.2021.744914

Cited by 9 publications

(3 citation statements)

References 36 publications

(55 reference statements)

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“…Many β-glucosidases that can hydrolyze ST have been reported previously; for example, BGL1, 25 EcBgl, 26 and CsBGL 27 can catalyze conversion of ST to RU. SPGase hydrolyzed ST to produce RU, steviolbioside, steviolmonoside and SV, but the yield of SV was only 64%.…”

Section: Resultsmentioning

confidence: 92%

A strategy to increase rebaudioside A content based on one-step bioconversion of Stevia extract to steviol

Wen

Lan

et al. 2023

Green Chem.

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Section: Resultsmentioning

confidence: 92%

A strategy to increase rebaudioside A content based on one-step bioconversion of Stevia extract to steviol

Wen

Lan

et al. 2023

Green Chem.

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“…Previous studies on the hydrolysis and modification of steviol glycosides mainly focused on ST and RA, while there were few reports on RC. It has been reported that some β-glucosidase and β-galactosidase from microorganisms hydrolyze ST and RA to STE ( Nguyen et al, 2019 ), demonstrating their ability to hydrolyze the β-glucosidic linkage of the C13 side chain and β-glucosyl ester linkage of C19 side chain ( Chen et al, 2014 ; Nguyen et al, 2016 ), although the substrate specificity of enzymes from different sources varies greatly ( Lan et al, 2019 ; Yan et al, 2021 ). Based on sequence homology, the proteins encoded by 1932, 1940, 1941, and 1944 genes belong to β-glucosidase of glycoside hydrolase family 3.…”

Section: Discussionmentioning

confidence: 99%

Integrated metabolite profiling and transcriptome analysis unraveling mechanism of RC catabolism in Paenarthrobacter ilicis CR5301

Li¹,

Sun²,

Cao³

et al. 2023

Front. Microbiol.

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Steviol glycosides are ideal sweeteners that are widely used in food, medicine, and cosmetics. Rebaudioside C (RC) is considered to be the third most abundant steviol glycoside, which has a bitter aftertaste that limits its application. Hydrolysis of RC to generate other bioactive steviol glycosides is an effective way to promote its additional utilization. In our previous study, a bacterium Paenarthrobacter ilicis CR5301 was isolated and identified for hydrolyzing RC with high efficiency. Herein, the expression profiles of P. ilicis CR5301 in the deletion and presence of RC were investigated by RNA-seq. The RC metabolites were identified by high-performance liquid chromatography and ultra-performance liquid chromatography-triple-time of flight mass spectrometry. Novel results were discovered in four aspects of research. First, the identification of metabolites revealed that four metabolites, namely, dulcoside A, dulcoside B, dulcoside A1, and steviol, were produced during RC metabolism. Second, RNA-seq analyses unraveled that 105 genes of P. ilicis CR5301 were significantly differentially expressed, and 7 pathways were significantly enriched. Third, independent RT-qPCR verified the accuracy and reliability of the RNA-seq results. Finally, a complete catabolic model of RC in P. ilicis CR5301 was proposed, and key genes were indicated in the RC catabolic metabolism by combining them with literature and sequence alignments. This study comprehensively unraveled the genes and pathways of RC catabolism in P. ilicis CR5301 at the transcriptional and metabolic levels. It provided new insights and evidence for understanding the mechanism of RC catabolism in bacteria. Key candidate genes may potentially contribute to the RC hydrolysis and preparation of other functional steviol glycosides in the future.

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“…More efficient methods are the heterologous gene expression of glycosyltransferases for steviol glycosylation or the production of rubusoside from Stevia extracts by enzymatic conversion using suitable glycosidases (Figure 4). Yan et al [38] isolated a β-glucosidase from Chryseobacterium scophthalmum 1433, which converted 99% of the applied steviol glycosides into rubusoside. Industrial applications of these methods are future aims that will obviate the need for Rubus suavissimus plant culture systems.…”

Section: Rubus Suavissimusmentioning

confidence: 99%

Culturing Important Plants for Sweet Secondary Products under Consideration of Environmentally Friendly Aspects

2022

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Some sweet tasting plant secondary metabolites are non-caloric or low nutritive compounds that have traditional use in food formulations. This mini-review focuses on conventional and advanced cultivation regimes of plants that accumulate sweet tasting or sweet taste modulating secondary metabolites of potential economic importance, in particular mogrosides (Siraitia grosvenorii), phyllodulcin (Hydrangea macrophylla), glycyrrhizin (Glycyrrhiza glabra), steviol glycosides (Stevia grosvenorii), and rubusoside (Rubus suavissimus). Consequential obstacles during the cultivation of Hydrangea macrophylla cultivars outside their natural habitat in a protected cultivation environment are addressed. Culturing at non-habitat locations facilitates short transportation routes of plant material for processing, which can be a key to an economically and environmentally compatible usage. The biosynthetic pathways, as far as known, are shortly mentioned. The proved or hypothetical degradation pathways of the compounds to minimalize environmental contamination are another focal point.

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A Novel β-Glucosidase From Chryseobacterium scophthalmum 1433 for Efficient Rubusoside Production From Stevioside

Cited by 9 publications

References 36 publications

A strategy to increase rebaudioside A content based on one-step bioconversion of Stevia extract to steviol

A strategy to increase rebaudioside A content based on one-step bioconversion of Stevia extract to steviol

Integrated metabolite profiling and transcriptome analysis unraveling mechanism of RC catabolism in Paenarthrobacter ilicis CR5301

Culturing Important Plants for Sweet Secondary Products under Consideration of Environmentally Friendly Aspects

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