A novel zinc finger protein–based amperometric biosensor for miRNA determination

Povedano, Eloy; Montiel, Víctor Ruiz‐Valdepeñas; Gamella, María; Serafín, Verónica; Pedrero, Marı́a; Moráňová, Ludmila; Bartošík, Martin; Montoya, Juan José; Yáñez‐Sedeño, Paloma; Campuzano, Susana; Pingarrón, José M.

doi:10.1007/s00216-019-02219-w

Cited by 26 publications

(5 citation statements)

References 49 publications

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“…One of the strategies to overcome the miR-21 short length issue is using zinc finger proteins (ZFPs). 31 A zinc finger is a small peptide with a specific secondary structure that is stabilized via the binding of zinc ions with histidines and two cysteines to bind miRNAs. 32 Povedano et al 31 designed a biosensor based on ZFP biorecognition for capturing the RNA hybrid formed between miR-21 molecules and achieved a LOD of 0.91 nM.…”

Section: Bioanalytical Systemsmentioning

confidence: 99%

“…31 A zinc finger is a small peptide with a specific secondary structure that is stabilized via the binding of zinc ions with histidines and two cysteines to bind miRNAs. 32 Povedano et al 31 designed a biosensor based on ZFP biorecognition for capturing the RNA hybrid formed between miR-21 molecules and achieved a LOD of 0.91 nM. Hybridization chain reaction (HCR), strand displacement amplification (SDA), loop-mediated isothermal amplification (LAMP), rolling circle amplification (RCA), and target-catalyzed hairpin assembly (CHA) are some isothermal nucleic acid amplification techniques developed for miRNA detection.…”

Section: Bioanalytical Systemsmentioning

confidence: 99%

See 1 more Smart Citation

Recent advances on the electrochemical and optical biosensing strategies for monitoring microRNA-21: a review

Esmaeilzadeh¹,

Yaseen

Khudaynazarov

et al. 2022

Anal. Methods

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Section: Bioanalytical Systemsmentioning

confidence: 99%

Section: Bioanalytical Systemsmentioning

confidence: 99%

Recent advances on the electrochemical and optical biosensing strategies for monitoring microRNA-21: a review

Esmaeilzadeh¹,

Yaseen

Khudaynazarov

et al. 2022

Anal. Methods

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“…The linear range was 4-50 fM with a linear regression equation of I (µA) = −9.81 logc (fM) + 4.26 (R 2 = 0.992), and the LOD was down to 120 aM (S/N = 3). The detection performance was comparable to and even better than most reported nanomaterials-based electrochemical assays for miRNA detection (Table 2) [26,[33][34][35][36][37] but without sophisticated electrode modifications. The high sensitivity of this method can be attributed to the three amplification strategies, including magnetic enrichment, enzymatic polymerization, and the use of Cu 2+ ions in SWV.…”

Section: Calibration Curves For Mirnas Detectionmentioning

confidence: 60%

Attomole Electrochemical Detection of MicroRNAs Based on Surface-Initiated Enzymatic Polymerization Coupled with Copper Enhancement

Zhu,

Xu,

Zhang

et al. 2023

Targets

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The sensitive and effective detection of microRNAs (miRNAs) is of great significance since miRNAs have been proven to have undeniable importance in participating in many biological processes. Herein, we present a novel, sensitive, label-free electrochemical miRNA detection method. Three signal amplification techniques are incorporated in this method, including the efficient conjugate of primer-modified polystyrene spheres (PS) with magnetic beads (MBs) triggered by target miRNA, template-free surface-initiated enzymatic polymerization (SIEP) on the primers, and the use of copper ions in square wave voltammetry (SWV) for detecting acidically depurinated primers. Cooperating with the electrochemical approach, this method was able to achieve a detection limit of 120 aM. With an attomole level of sensitivity and easiness of manipulation, this novel method is suitable for miRNA routine detection in both research and clinical aspects.

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“…This happens because the interactivity exhibited by the hexahistidine tag (H6) and other His-rich tails with Ni 2+ during the immobilized metal affinity chromatographic (IMAC) purification of recombinant proteins [2,3] can be also exploited for a controlled crossmolecular assembly, when adding defined amounts of Zn 2+ , Ca 2+ , Mn 2+ or other divalent cations to solutions of pure Histagged protein [4][5][6]. In fact, this principle sustains the formation of the secretory granules in the mammalian endocrine system [7,8] and of different types of amyloidal and non-amyloidal protein materials existing in nature [4,7,[9][10][11][12][13][14][15][16]. The simplicity of His-rich peptides used as architectonic agents at the nanoscale offers an interesting alternative to more refined approaches to control protein self-assembling, that might require more sophisticated protein engineering [17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

The spectrum of building block conformers sustains the biophysical properties of clinically-oriented self-assembling protein nanoparticles

Voltà‐Durán

Sánchez²,

Hèctor-López-Laguna³

et al. 2022

Sci. China Mater.

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Histidine-rich peptides confer self-assembling properties to recombinant proteins through the supramolecular coordination with divalent cations. This fact allows the cost-effective, large-scale generation of microscopic and macroscopic protein materials with intriguing biomedical properties. Among such materials, resulting from the simple bioproduction of protein building blocks, homomeric nanoparticles are of special value as multivalent interactors and drug carriers. Interestingly, we have here identified that the assembly of a given His-tagged protein might render distinguishable categories of self-assembling protein nanoparticles. This fact has been scrutinized through the nanobody-containing fusion proteins EM1-GFP-H6 and A3C8-GFP-H6, whose biosynthesis results in two distinguishable populations of building blocks. In one of them, the assembling and disassembling is controllable by cations. However, a second population immediately self-assembles upon purification through a non-regulatable pathway, rendering larger nanoparticles with specific biological properties. The structural analyses of both model proteins and nanoparticles revealed important conformational variability in the building blocks. This fact renders different structural and functional categories of the final soft materials resulting from the participation of energetically unstable intermediates in the oligomerization process. These data illustrate the complexity of the Hismediated protein assembling in recombinant proteins but they also offer clues for a better design and refinement of protein-based nanomedicines, which, resulting from biological fabrication, show an architectonic flexibility unusual among biomaterials.

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A novel zinc finger protein–based amperometric biosensor for miRNA determination

Cited by 26 publications

References 49 publications

Recent advances on the electrochemical and optical biosensing strategies for monitoring microRNA-21: a review

Recent advances on the electrochemical and optical biosensing strategies for monitoring microRNA-21: a review

Attomole Electrochemical Detection of MicroRNAs Based on Surface-Initiated Enzymatic Polymerization Coupled with Copper Enhancement

The spectrum of building block conformers sustains the biophysical properties of clinically-oriented self-assembling protein nanoparticles

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