A novel WRKY34-bZIP3 module regulates phenolic acid and tanshinone biosynthesis in Salvia miltiorrhiza

Shi, Min; Zhu, Ruiyan; Zhang, Yi; Zhang, Siwei; Liu, Tingyao; Li, Kunlun; Liu, Shucan; Wang, Leran; Wang, Yao; Zhou, Wei; Hua, Qiang; Kai, Guoyin

doi:10.1016/j.ymben.2022.08.002

Cited by 25 publications

(10 citation statements)

References 50 publications

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“…The TAT is the initial enzyme in the tyrosine-derived pathway, and overexpression of SmTAT1 was able to significantly increase the phenolic acid content in S. miltiorrhiza [ 5 ]. As a critical enzyme gene in phenolic acid biosynthesis pathway, SmTAT1 had been shown to be a targeted gene of various TFs, including SmbZIP3 and SmbHLH60 [ 26 , 27 ]. Indeed, the as-1 element was found in the SmTAT1 promoter.…”

Section: Discussionmentioning

confidence: 99%

The SmNPR4-SmTGA5 module regulates SA-mediated phenolic acid biosynthesis in Salvia miltiorrhiza hairy roots

Ding

Zhang

et al. 2023

Horticulture Research

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Phenolic acids are the main bioactive compounds in Salvia miltiorrhiza, which can be increased by salicylic acid (SA) elicitation. However, the specific molecular mechanism remains unclear. The nonexpresser of PR genes 1 (NPR1) and its family members are essential components of the SA signaling pathway. Here, we report an NPR protein, SmNPR4, showed strongly expression in hairy root after SA treatment, acting as a negative moderator of SA-induced phenolic acid biosynthesis in Salvia miltiorrhiza (S. miltiorrhiza). Moreover, a basic leucine zipper family transcription factor SmTGA5 was identified and was found to interact with SmNPR4. SmTGA5 activates the expression of phenolic acid biosynthesis gene SmTAT1 through binding to the as-1 element. Finally, a series of biochemical assays and dual gene overexpression analysis demonstrated that the SmNPR4 significantly inhibited the function of SmTGA5, and SA can alleviate the inhibitory effect of SmNPR4 on SmTGA5. Overall, our results reveal the molecular mechanism of salicylic acid regulating phenolic acid biosynthesis in S. miltiorrhiza and provides new insights for SA signaling to regulate secondary metabolic biosynthesis.

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Section: Discussionmentioning

confidence: 99%

The SmNPR4-SmTGA5 module regulates SA-mediated phenolic acid biosynthesis in Salvia miltiorrhiza hairy roots

Ding

Zhang

et al. 2023

Horticulture Research

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“…SmWRKY34, in high homology with Arabidopsis WRKY40, played a negative role in phenolic acid biosynthesis. Transcriptome analysis revealed that ABA‐responsive bZIP TF SmbZIP3 , was differentially regulated by SmWRKY34 , and further study indicated that SmbZIP3 exhibited a promotion of phenolic acid production by activating the key biosynthetic gene SmTAT1 (Shi et al, 2022a). In addition, SmbZIP2 appeared as a negative regulator in the biosynthesis of phenolic acids via repressing the SmPAL1 gene (Shi et al, 2021c).…”

Section: Transcriptional Regulation Of Value‐added Product Biosynthes...mentioning

confidence: 99%

“…SmMYB9b and SmMYB98 also promoted tanshinone biosynthesis (Zhang et al, 2017b; Hao et al, 2020). Overexpression of ABA‐inducible SmbZIP3 significantly enhanced tanshionone content in S. miltiorrhiza hairy roots by activating SmERF128 and SmMYB9b, while introduction of SmWRKY34 decreased tanshinone by downregulating expression of SmbZIP3 and SmGGPPS (Shi et al, 2022a).…”

Section: Transcriptional Regulation Of Value‐added Product Biosynthes...mentioning

confidence: 99%

Molecular regulation of the key specialized metabolism pathways in medicinal plants

Shi,

Zhang,

Zheng

et al. 2024

JIPB

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The basis of modern pharmacology is the human ability to exploit the production of specialized metabolites from medical plants, for example, terpenoids, alkaloids, and phenolic acids. However, in most cases, the availability of these valuable compounds is limited by cellular or organelle barriers or spatio‐temporal accumulation patterns within different plant tissues. Transcription factors (TFs) regulate biosynthesis of these specialized metabolites by tightly controlling the expression of biosynthetic genes. Cutting‐edge technologies and/or combining multiple strategies and approaches have been applied to elucidate the role of TFs. In this review, we focus on recent progress in the transcription regulation mechanism of representative high‐value products and describe the transcriptional regulatory network, and future perspectives are discussed, which will help develop high‐yield plant resources.

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“…For example, in Salvia miltiorrhiza , SmWRKY34 has been shown to have a negative effect on phenolic acids and tanshinones by directly regulating SmRAS and SmGGPPS [ 8 ]. Similarly, overexpression of SmWRKY2 in S. miltiorrhiza hairy roots has been found to significantly enhance the expression of SmDXS2 and SmCPS , resulting in an increased accumulation of tanshinones [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

PatWRKY71 transcription factor regulates patchoulol biosynthesis and plant defense response

Li,

Huang,

Zuo

et al. 2024

BMC Plant Biol

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Patchoulol, a valuable compound belonging to the sesquiterpenoid family, is the primary component of patchouli oil produced by Pogostemon cablin (P. cablin). It has a variety of pharmacological and biological activities and is widely used in the medical and cosmetic industries. However, despite its significance, there is a lack of research on the transcriptional modulation of patchoulol biosynthesis.Salicylic acid (SA), is a vital plant hormone that serves as a critical signal molecule and plays an essential role in plant growth and defense. However, to date, no studies have explored the modulation of patchoulol biosynthesis by SA. In our study, we discovered that the application of SA can enhance the production of patchoulol. Utilizing transcriptome analysis of SA-treated P. cablin, we identified a crucial downstream transcription factor, PatWRKY71. The transcription level of PatWRKY71 was significantly increased with the use of SA. Furthermore, our research has revealed that PatWRKY71 was capable of binding to the promoter of PatPTS, ultimately leading to an increase in its expression. When PatWRKY71 was silenced by a virus, the expression of both PatWRKY71 and PatPTS was reduced, resulting in the down-regulation of patchoulol production. Through our studies, we discovered that heterologous expression of PatWRKY71 leads to an increase in the sensitivity of Arabidopsis to salt and Cd, as well as an outbreak of reactive oxygen species (ROS). Additionally, we uncovered the regulatory role of PatWRKY71 in both patchoulol biosynthesis and plant defense response. This discovery provided a theoretical basis for the improvement of the content of patchoulol and the resistance of P. cablin through genetic engineering.

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A novel WRKY34-bZIP3 module regulates phenolic acid and tanshinone biosynthesis in Salvia miltiorrhiza

Cited by 25 publications

References 50 publications

The SmNPR4-SmTGA5 module regulates SA-mediated phenolic acid biosynthesis in Salvia miltiorrhiza hairy roots

The SmNPR4-SmTGA5 module regulates SA-mediated phenolic acid biosynthesis in Salvia miltiorrhiza hairy roots

Molecular regulation of the key specialized metabolism pathways in medicinal plants

PatWRKY71 transcription factor regulates patchoulol biosynthesis and plant defense response

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