A Novel WRKY Transcription Factor Is Required for Induction of <i>PR-1a</i> Gene Expression by Salicylic Acid and Bacterial Elicitors

Verk, Marcel C. Van; Pappaioannou, Dimitri; Neeleman, Lyda; Bol, John F.; Linthorst, Huub J. M.

doi:10.1104/pp.107.112789

Cited by 236 publications

(163 citation statements)

References 52 publications

Supporting

Mentioning

146

Contrasting

Unclassified

Order By: Relevance

“…However, several studies have revealed that some WRKY proteins can bind to non-W or non-W-like box ciselements (Sun et al, 2003;Cai et al, 2008;van Verk et al, 2008). For example, a Suc-regulated barley (Hordeum vulgare) WRKY transcription factor, SUGAR SIG-NALING IN BARLEY2, can bind to both the W box and a sugar-responsive element (Sun et al, 2003).…”

Section: Wrky13 Specifically Binds To Multiple Types Of Cis-elements mentioning

confidence: 99%

Rice WRKY13 Regulates Cross Talk between Abiotic and Biotic Stress Signaling Pathways by Selective Binding to Different cis-Elements

et al. 2013

View full text Add to dashboard Cite

Plants use a complex signal transduction network to regulate their adaptation to the ever-changing environment. Rice (Oryza sativa) WRKY13 plays a vital role in the cross talk between abiotic and biotic stress signaling pathways by suppressing abiotic stress resistance and activating disease resistance. However, it is not clear how WRKY13 directly regulates this cross talk. Here, we show that WRKY13 is a transcriptional repressor. During the rice responses to drought stress and bacterial infection, WRKY13 selectively bound to certain site-and sequence-specific cis-elements on the promoters of SNAC1 (for STRESS RESPONSIVE NO APICAL MERISTEM, ARABIDOPSIS TRANSCRIPTION ACTIVATION FACTOR1/2, CUP-SHAPED COTYLEDON), the overexpression of which increases drought resistance, and WRKY45-1, the knockout of which increases both bacterial disease and drought resistance. WRKY13 also bound to two cis-elements of its native promoter to autoregulate the balance of its gene expression in different physiological activities. WRKY13 was induced in leaf vascular tissue, where bacteria proliferate, during infection, and in guard cells, where the transcriptional factor SNAC1 enhances drought resistance, during both bacterial infection and drought stress. These results suggest that WRKY13 regulates the antagonistic cross talk between drought and disease resistance pathways by directly suppressing SNAC1 and WRKY45-1 and autoregulating its own expression via site-and sequence-specific cis-elements on the promoters of these genes in vascular tissue where bacteria proliferate and guard cells where the transcriptional factor SNAC1 mediates drought resistance by promoting stomatal closure.

show abstract

Section: Wrky13 Specifically Binds To Multiple Types Of Cis-elements mentioning

confidence: 99%

Rice WRKY13 Regulates Cross Talk between Abiotic and Biotic Stress Signaling Pathways by Selective Binding to Different cis-Elements

et al. 2013

View full text Add to dashboard Cite

show abstract

“…5). Previous studies have reported that a barley (Hordeum vulgare) WRKY protein binds to a sugarresponsive cis-element specifically and a tobacco (Nicotiana tabacum) WRKY binds to a WK-box (Sun et al, 2003;van Verk et al, 2008). Thus, further study is required to determine whether OsWRKY13 could regulate OsWRKY45-1 via binding to a non-W-box or non-W-box-like element.…”

Section: Oswrky45 and Oswrky13 May Regulate Each Other In A Feedback mentioning

confidence: 99%

A Pair of Allelic WRKY Genes Play Opposite Roles in Rice-Bacteria Interactions

et al. 2009

View full text Add to dashboard Cite

Although allelic diversity of genes has been reported to play important roles in different physiological processes, information on allelic diversity of defense-responsive genes in host-pathogen interactions is limited. Here, we report that a pair of allelic genes, OsWRKY45-1 and OsWRKY45-2, which encode proteins with a 10-amino acid difference, play opposite roles in rice (Oryza sativa) resistance against bacterial pathogens. Bacterial blight caused by Xanthomonas oryzae pv oryzae (Xoo), bacterial streak caused by Xanthomonas oryzae pv oryzicola (Xoc), and fungal blast caused by Magnaporthe grisea are devastating diseases of rice worldwide. OsWRKY45-1-overexpressing plants showed increased susceptibility and OsWRKY45-1-knockout plants showed enhanced resistance to Xoo and Xoc. In contrast, OsWRKY45-2-overexpressing plants showed enhanced resistance and OsWRKY45-2-suppressing plants showed increased susceptibility to Xoo and Xoc. Interestingly, both OsWRKY45-1-and OsWRKY45-2-overexpressing plants showed enhanced resistance to M. grisea. OsWRKY45-1-regulated Xoo resistance was accompanied by increased accumulation of salicylic acid and jasmonic acid and induced expression of a subset of defenseresponsive genes, while OsWRKY45-2-regulated Xoo resistance was accompanied by increased accumulation of jasmonic acid but not salicylic acid and induced expression of another subset of defense-responsive genes. These results suggest that both OsWRKY45-1 and OsWRKY45-2 are positive regulators in rice resistance against M. grisea, but the former is a negative regulator and the latter is a positive regulator in rice resistance against Xoo and Xoc. The opposite roles of the two allelic genes in rice-Xoo interaction appear to be due to their mediation of different defense signaling pathways.

show abstract

“…Members of this family contain at least one conserved DNA-binding region, designated the WRKY domain, comprising the highly conserved WRKYGQK peptide sequence and a zinc finger motif (CX 4-7 CX 22-23 HXH/C). This domain generally binds to the DNA element termed the W box (C/TTGACT/C), although alternative binding sites have been identified (Sun et al, 2003;Cai et al, 2008;Ciolkowski et al, 2008;van Verk et al, 2008). WRKY family members are divided into three groups based on the number of WRKY domains and certain features of the zinc finger-like motifs (Eulgem et al, 2000).…”

Section: The Wrky Factorsmentioning

confidence: 99%