A novel workflow for unbiased quantification of autophagosomes in 3D inArabidopsis thalianaroots
Michal Daněk,
Daniela Kocourková,
Tereza Podmanická
et al.
Abstract:Macroautophagy is frequently quantified by live imaging of autophagosomes decorated with a marker of fluorescently tagged ATG8 protein (FT-ATG8) inArabidopsis thaliana. This requires generation of suitable plant material by time-consuming crossing or transformation with FT-ATG8 marker. Autophagosome quantification by image analysis often relies on their counting in individual focal planes. This approach is prone to deliver biased results due to inappropriate sampling of the regions of interest in the Z-directi… Show more
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