A novel widespread interkingdom signaling circuit

González, Juan F.; Venturi, Vittorio

doi:10.1016/j.tplants.2012.09.007

Cited by 119 publications

(126 citation statements)

References 74 publications

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“…Biologists now know that bacteria have social behaviors, communicating with each other through chemical signaling, such as quorum sensing (67,68); more recently, interdomain quorum signaling between bacteria and their eukaryotic partners has become evident (22,(69)(70)(71). In addition to quorum signals, bacteria use cell surfacederived molecules to communicate with their hosts, affecting host processes both at the cellular level [e.g., apoptosis, Toll-like receptor (TLR) signaling (52,72)], as well as at the organ-system level (Fig.…”

Section: Interdomain Communicationmentioning

confidence: 99%

Animals in a bacterial world, a new imperative for the life sciences

McFall‐Ngai

Hadfield

Bosch

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

2,197

1,899

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In the last two decades, the widespread application of genetic and genomic approaches has revealed a bacterial world astonishing in its ubiquity and diversity. This review examines how a growing knowledge of the vast range of animal–bacterial interactions, whether in shared ecosystems or intimate symbioses, is fundamentally altering our understanding of animal biology. Specifically, we highlight recent technological and intellectual advances that have changed our thinking about five questions: how have bacteria facilitated the origin and evolution of animals; how do animals and bacteria affect each other’s genomes; how does normal animal development depend on bacterial partners; how is homeostasis maintained between animals and their symbionts; and how can ecological approaches deepen our understanding of the multiple levels of animal–bacterial interaction. As answers to these fundamental questions emerge, all biologists will be challenged to broaden their appreciation of these interactions and to include investigations of the relationships between and among bacteria and their animal partners as we seek a better understanding of the natural world

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Section: Interdomain Communicationmentioning

confidence: 99%

Animals in a bacterial world, a new imperative for the life sciences

McFall‐Ngai

Hadfield

Bosch

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

2,197

1,899

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“…Sequence alignments revealed that the reported nine highly conserved amino acid residues shared by most LuxR-type proteins are included in NmuR (Fig. 1D) (26). The I/R QS system is required for activation by LuxR and its homologs, and the active form of these transcription factors requires their cognate signals or closely related AHLs (17,27).…”

Section: Characterization Of the Novel Luxi Homolog Nmuimentioning

confidence: 99%

An N -Acyl Homoserine Lactone Synthase in the Ammonia-Oxidizing Bacterium Nitrosospira multiformis

Gao

Zhuang

et al. 2014

Appl Environ Microbiol

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The chemolithoautotrophic bacterium Nitrosospira multiformis is involved in affecting the process of nitrogen cycling. Here we report the existence and characterization of a functional quorum sensing signal synthase in N. multiformis. One gene (nmuI) playing a role in generating a protein with high levels of similarity to N-acyl homoserine lactone (AHL) synthase protein families was identified. Two AHLs (C 14 -AHL and 3-oxo-C 14 -AHL) were detected using an AHL biosensor and liquid chromatographymass spectrometry (LC-MS) when nmuI, producing a LuxI homologue, was introduced into Escherichia coli. However, by extracting N. multiformis culture supernatants with acidified ethyl acetate, no AHL product was obtained that was capable of activating the biosensor or being detected by LC-MS. According to reverse transcription-PCR, the nmuI gene is transcribed in N. multiformis, and a LuxR homolog (NmuR) in this ammonia-oxidizing strain showed great sensitivity to long-chain AHL signals by solubility assay. A degradation experiment demonstrated that the absence of AHL signals might be attributed to the possible AHL-inactivating activities of this strain. To summarize, an AHL synthase gene (nmuI) acting as a long-chain AHL producer has been found in a chemolithotrophic ammonia-oxidizing microorganism, and the results provide an opportunity to complete the knowledge of the regulatory networks in N. multiformis. Q uorum sensing (QS) is a form of cell-cell communication that regulates gene expression in response to fluctuations in cell density. QS bacteria can alter their behavior through producing, releasing, and responding to autoinducing signaling molecules that accumulate in the environment (1). A variety of physiological functions, including biofilm formation, bioluminescence, virulence, swarming, plasmid transfer, and antibiotic biosynthesis, are subject to QS regulation (1-3). In Gram-negative bacteria, several signal molecules have now been identified, such as N-acyl homoserine lactones (AHLs), quinolone, p-coumarate, and 3-OH palmitic acid methyl ester (3-OH PAME), and the AHLs have probably been the most intensively investigated of these (3, 4). In the currently accepted LuxI/LuxR-type regulatory system of QS, AHL biosynthesis depends primarily on a synthase protein (I protein), and target genes are then activated via the interaction between the signal molecules and a response regulator protein (R protein) (1,3,5).Nitrosospira multiformis is a chemolithoautotrophic bacterium that is capable of oxidizing ammonia to obtain energy for growth (6). The ecological importance of N. multiformis and other ammonia-oxidizing bacteria (AOB) is that they affect the biological oxidation of inorganic nitrogen compounds in the environment. During the ammonia oxidation process in water or soil, biofilm formed by AOB can greatly affect the nitrification efficiency and ecological behavior of nitrifying bacteria (7-9). In many Gramnegative bacteria, the QS process controls exopolysaccharide production and biofilm formation, which is me...

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“…For example, Escherichia coli and Salmonella enterica serovar Typhimurium possess a luxR homolog called sdiA, which codes for a transcription factor responsive to AHLs produced by other bacteria (12). Some luxR homologs in plant-associated bacteria code for transcription factors that respond to plant-derived signals of unknown composition rather than AHLs (reviewed in reference 13).…”

mentioning

confidence: 99%

LuxR- and LuxI-Type Quorum-Sensing Circuits Are Prevalent in Members of the Populus deltoides Microbiome

Schaefer

Lappala

Morlen

et al. 2013

Appl Environ Microbiol

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We are interested in the root microbiome of the fast-growing Eastern cottonwood tree, Populus deltoides. There is a large bank of bacterial isolates from P. deltoides, and there are 44 draft genomes of bacterial endophyte and rhizosphere isolates. As a first step in efforts to understand the roles of bacterial communication and plant-bacterial signaling in P. deltoides, we focused on the prevalence of acyl-homoserine lactone (AHL) quorum-sensing-signal production and reception in members of the P. deltoides microbiome. We screened 129 bacterial isolates for AHL production using a broad-spectrum bioassay that responds to many but not all AHLs, and we queried the available genome sequences of microbiome isolates for homologs of AHL synthase and receptor genes. AHL signal production was detected in 40% of 129 strains tested. Positive isolates included members of the Alpha-, Beta-, and Gammaproteobacteria. Members of the luxI family of AHL synthases were identified in 18 of 39 proteobacterial genomes, including genomes of some isolates that tested negative in the bioassay. Members of the luxR family of transcription factors, which includes AHL-responsive factors, were more abundant than luxI homologs. There were 72 in the 39 proteobacterial genomes. Some of the luxR homologs appear to be members of a subfamily of LuxRs that respond to as-yet-unknown plant signals rather than bacterial AHLs. Apparently, there is a substantial capacity for AHL cell-to-cell communication in proteobacteria of the P. deltoides microbiota, and there are also Proteobacteria with LuxR homologs of the type hypothesized to respond to plant signals or cues.

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A novel widespread interkingdom signaling circuit

Cited by 119 publications

References 74 publications

Animals in a bacterial world, a new imperative for the life sciences

Animals in a bacterial world, a new imperative for the life sciences

An N -Acyl Homoserine Lactone Synthase in the Ammonia-Oxidizing Bacterium Nitrosospira multiformis

LuxR- and LuxI-Type Quorum-Sensing Circuits Are Prevalent in Members of the Populus deltoides Microbiome

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