A Novel Virus–Host Cell Membrane Interaction

Poranen, Minna M.; Daugelavičius, Rimantas; Ojala, Päivi M.; Hess, Michael W.; Bamford, Dennis H.

doi:10.1083/jcb.147.3.671

Cited by 40 publications

(13 citation statements)

References 71 publications

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“…The loss of viral membrane around the NC enables the muralytic (peptidoglycan-degrading) enzyme P5, located on the NC surface, to degrade the peptidoglycan layer of the bacterial cell wall [ 49 , 50 ]. The permeabilization of the host plasma membrane facilitates the translocation of the NC across the cytoplasmic membrane of the host cell through an endocytosis-like process, driven by P8 [ 51 , 52 ]. Upon entry into the cytoplasm, the P8 shell of the NC dissociates to reveal the naked dodecahedral polymerase complex (PC).…”

Section: Cystoviridaementioning

confidence: 99%

RNA Phage Biology in a Metagenomic Era

Callanan

Stockdale

Shkoporov

et al. 2018

Viruses

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The number of novel bacteriophage sequences has expanded significantly as a result of many metagenomic studies of phage populations in diverse environments. Most of these novel sequences bear little or no homology to existing databases (referred to as the “viral dark matter”). Also, these sequences are primarily derived from DNA-encoded bacteriophages (phages) with few RNA phages included. Despite the rapid advancements in high-throughput sequencing, few studies enrich for RNA viruses, i.e., target viral rather than cellular fraction and/or RNA rather than DNA via a reverse transcriptase step, in an attempt to capture the RNA viruses present in a microbial communities. It is timely to compile existing and relevant information about RNA phages to provide an insight into many of their important biological features, which should aid in sequence-based discovery and in their subsequent annotation. Without comprehensive studies, the biological significance of RNA phages has been largely ignored. Future bacteriophage studies should be adapted to ensure they are properly represented in phageomic studies.

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Section: Cystoviridaementioning

confidence: 99%

RNA Phage Biology in a Metagenomic Era

Callanan

Stockdale

Shkoporov

et al. 2018

Viruses

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“…identified a lipid-containing bacteriophage, phi6, with a three-segmented dsRNA genome3. The characterization of these viruses has improved the understanding of dsRNA virus biology, such as its replication, virus assembly, genome packaging, and phage–host interactions456789. All dsRNA phages are classified in the Cystoviridae family; phi6 is a representative member of Cystoviridae.…”

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confidence: 99%

Characterization of the first double-stranded RNA bacteriophage infecting Pseudomonas aeruginosa

Yang

Shen

et al. 2016

Sci Rep

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Bacteriophages (phages) are widely distributed in the biosphere and play a key role in modulating microbial ecology in the soil, ocean, and humans. Although the role of DNA bacteriophages is well described, the biology of RNA bacteriophages is poorly understood. More than 1900 phage genomes are currently deposited in NCBI, but only 6 dsRNA bacteriophages and 12 ssRNA bacteriophages genome sequences are reported. The 6 dsRNA bacteriophages were isolated from legume samples or lakes with Pseudomonas syringae as the host. Here, we report the first Pseudomonas aeruginosa phage phiYY with a three-segmented dsRNA genome. phiYY was isolated from hospital sewage in China with the clinical P. aeruginosa strain, PAO38, as a host. Moreover, the dsRNA phage phiYY has a broad host range, which infects 99 out of 233 clinical P. aeruginosa strains isolated from four provinces in China. This work presented a detailed characterization of the dsRNA bacteriophage infecting P. aeruginosa.

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“…These data were accompanied by tracking intra- and extracellular ATP changes [ 48 ]. While this potentiometric approach has been previously used to study the life cycles of several phages in moderate environments [ 48 , 49 , 50 , 51 , 52 , 53 ], here we demonstrate that these techniques can also be applied to viruses thriving in extreme salinities (>3 M NaCl).…”

Section: Introductionmentioning

confidence: 69%

Monitoring Physiological Changes in Haloarchaeal Cell during Virus Release

Svirskaite

Oksanen

Daugelavičius

et al. 2016

Viruses

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The slow rate of adsorption and non-synchronous release of some archaeal viruses have hindered more thorough analyses of the mechanisms of archaeal virus release. To address this deficit, we utilized four viruses that infect Haloarcula hispanica that represent the four virion morphotypes currently known for halophilic euryarchaeal viruses: (1) icosahedral internal membrane-containing SH1; (2) icosahedral tailed HHTV-1; (3) spindle-shaped His1; and (4) pleomorphic His2. To discern the events occurring as the progeny viruses exit, we monitored culture turbidity, as well as viable cell and progeny virus counts of infected and uninfected cultures. In addition to these traditional metrics, we measured three parameters associated with membrane integrity: the binding of the lipophilic anion phenyldicarbaundecaborane, oxygen consumption, and both intra- and extra-cellular ATP levels.

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A Novel Virus–Host Cell Membrane Interaction

Cited by 40 publications

References 71 publications

RNA Phage Biology in a Metagenomic Era

RNA Phage Biology in a Metagenomic Era

Characterization of the first double-stranded RNA bacteriophage infecting Pseudomonas aeruginosa

Monitoring Physiological Changes in Haloarchaeal Cell during Virus Release

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