A novel variant of translation elongation factor-1β: isolation and characterization of the rice gene encoding EF-1β2

Terui, Yoshiaki; Tsutsumi, Ken‐ichi; Kidou, Shin‐ichiro; Sawazaki, Takeshi; Kuroiwa, Yasuyuki; Yamaki, Masahiro; Ejiri, Shin-ichiro

doi:10.1016/s0167-4781(98)00153-5

Cited by 7 publications

(9 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4). Interestingly, the 5¢ UTRs of the rice rps10 genes showed 63±73% identity to those of various rice transcripts, such as those derived Nucleotide sequence alignment of the 5¢¯anking regions of rice s10-1 (this study), EF-1b2 (Terui et al 1998), and the rice genomic DNA clones #15 and #18 (this study). Nucleotides identical to the s10-1 are indicated by asterisks.…”

Section: Genomic Organization Of the Rps10 Genes In Ricementioning

confidence: 71%

“…The GT dinucleotides at the 5¢ border of the introns are indicated by the black bar and the intron sequences are given in lower case letters. The complete intron sequences are not shown here but their lengths are indicated (in parentheses) at the end of the last row from the rice genes for the SNF1-like protein kinase Osk4 (Takano et al 1998), elongation factor EF-1b2 (Terui et al 1998), the G protein a subunit RGA1 (Seo et al 1995) and several deduced from EST clones (data not shown). In particular, #15, #18 and the EF-1b2 gene each have an intron located at similar position that shows a high degree of sequence similarity to those of rice rps10 genes (Fig.…”

Section: Genomic Organization Of the Rps10 Genes In Ricementioning

confidence: 99%

See 1 more Smart Citation

Transfer of the mitochondrial rps10 gene to the nucleus in rice: acquisition of the 5′ untranslated region followed by gene duplication

et al. 2000

View full text Add to dashboard Cite

Mitochondrial ribosomal protein S10 (rps10) is encoded by the mitochondrial genome in potato and pea. Here we show that the rps10 gene is absent from the mitochondrial genome of rice and has been transferred to the nucleus. Cloning and transcriptional analysis show that there are two rps10 genes in the rice nuclear genome and that their transcripts differ in abundance. Western analysis detected the RPS10 protein in the soluble fraction of rice mitochondria, although neither RPS10 has any obvious N-terminal presequence for targeting to mitochondria. This result suggests that targeting information is present in the internal region of rice RPS10. Genomic sequence analysis indicated that each rps10 gene has an intron in the 5' untranslated region (5' UTR) and that these intron sequences are homologous to each other. This result strongly suggests that a duplication event occurred after transfer of the rps10 gene to the nucleus. The duplicated rps10 genes have since been translocated to different chromosomes, because the two rps10 genes were mapped on chromosomes 6 and 12 by RFLP analysis. Interestingly, the 5' UTR and the intron of the rice rps10 genes are homologous to sequences found in several rice genes with various functions, such as osk4, EF-1beta2 and RAG1, suggesting a common origin and a functional role for the 5' UTR. Acquisition of the 5' flanking region might have accelerated the activation of the mitochondrial rps10 gene which was transferred to the nuclear genome.

show abstract

Section: Genomic Organization Of the Rps10 Genes In Ricementioning

confidence: 71%

Section: Genomic Organization Of the Rps10 Genes In Ricementioning

confidence: 99%

Transfer of the mitochondrial rps10 gene to the nucleus in rice: acquisition of the 5′ untranslated region followed by gene duplication

et al. 2000

View full text Add to dashboard Cite

show abstract

“…cDNAs encoding eEF1B subunits were provided by Shin-Ichiro Kidou (Iwate University). The cDNAs for rice eEF1Bb and eEF1Bb2 (Terui et al 1998) were modified by PCR and subcloned into EcoRI/XhoI-digested pCITE-4a (Novagen). The cDNA encoding rice eEF1Ba was also modified and subcloned into EcoRI/SalI-digested pCITE-4a.…”

Section: Methodsmentioning

confidence: 99%

Mutation of a conserved CDK site converts a metazoan Elongation Factor 1Bβ subunit into a replacement for yeast eEF1Bα

et al. 2003

View full text Add to dashboard Cite

Elongation factor subunit eEF1Bbeta (formerly EF-1beta in plants and EF-1delta in animals) was identified and cloned in a screen for proteins from pea that interact with a cyclin-dependent kinase (CDK). CDKs are enzymes that regulate progression through meiotic and mitotic cell cycles in eukaryotes. eEF1Bbeta and the related protein eEF1Balpha (formerly EF-1beta' in plants and EF-1beta in animals and fungi) can catalyze GTP/GDP exchange on the G-protein eEF1A (formerly EF-1alpha in plants, animals and fungi) during the elongation phase of protein synthesis in eukaryotes. Recombinant Cdc2 and its native homologues from pea extracts associated both in vitro and in vivo with eEF1Bbeta. A Cdc2-cyclin B complex phosphorylated recombinant plant eEF1Bbetas, but not eEF1Balpha. These interactions between CDK and eEF1Bbeta prompted investigations into the in vivo consequences of this relationship. Expression of cDNAs encoding rice or pea eEF1Bbeta subunits failed to complement a Saccharomyces cerevisiae mutant deleted for the eEF1Balpha gene, as was previously observed for the human eEF1Bbeta. However, replacement of Thr91, the sole consensus CDK phosphorylation site in pea eEF1Bbeta, with alanine allowed the pea protein to substitute for eEF1Balpha function in vivo. In addition, this rescued strain was severely cold sensitive, and more sensitive to translational inhibitors than wild-type yeast. Taken together, these results suggest a physiological connection between the cyclin-dependent class of kinases and a translational elongation factor in mitotic cells, and provide the first in vivo evidence that an altered form of eEF1Bbeta can serve as the guanine nucleotide exchange factor for eEF1A.

show abstract

“…are ubiquitously expressed, the third similar EF-1b, EF-1b2, which has higher similarity to EF-1b, is speciˆcally expressed in rice seed and cultured cells. 32) Although homodimers of EF-Ts are detected, 33) rice EF-1b and EF-1b? form heterodimers, but not homodimers, and EF-1b but not EF-1b?, has the ability to form a complex with EF-1g.…”

Section: Structure and Functions Of Ef-1 And Ef-1 Genesmentioning

confidence: 99%

Moonlighting Functions of Polypeptide Elongation Factor 1: From Actin Bundling to Zinc Finger Protein R1-Associated Nuclear Localization

Ejiri

2002

Bioscience, Biotechnology, and Biochemistry

Self Cite

191

168

View full text Add to dashboard Cite

A novel variant of translation elongation factor-1β: isolation and characterization of the rice gene encoding EF-1β2

Cited by 7 publications

References 15 publications

Transfer of the mitochondrial rps10 gene to the nucleus in rice: acquisition of the 5′ untranslated region followed by gene duplication

Transfer of the mitochondrial rps10 gene to the nucleus in rice: acquisition of the 5′ untranslated region followed by gene duplication

Mutation of a conserved CDK site converts a metazoan Elongation Factor 1Bβ subunit into a replacement for yeast eEF1Bα

Moonlighting Functions of Polypeptide Elongation Factor 1: From Actin Bundling to Zinc Finger Protein R1-Associated Nuclear Localization

Contact Info

Product

Resources

About