A novel variant in the promoter region of miR-9 gene strongly affects litter size in Markhoz goats

Mahmoudi, Peyman; Rashidi, Amir; Rostamzadeh, Jalal; Razmkabir, Mohammad

doi:10.1016/j.theriogenology.2020.09.008

Cited by 6 publications

(5 citation statements)

References 25 publications

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“… 81 In this context, our results suggest that miR‐30c‐5p could act upon the endometrium regulating its receptivity for the embryo. Similarly, miR‐9‐5p could be involved in regulation of embryo development and/or endometrial receptivity based on the results of a genetic study in goats showing a strong association with the number of fetuses 82 and its expression in porcine endometrium during implantation. 83 , 84 For miR‐148a‐3p, a regulatory role for immune response in bovine endometrium by reducing inflammatory responses has been shown.…”

Section: Discussionmentioning

confidence: 99%

Dynamic regulation of the transcriptome and proteome of the equine embryo during maternal recognition of pregnancy

et al. 2022

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During initial maternal recognition of pregnancy (MRP), the equine embryo displays a series of unique events characterized by rapid blastocyst expansion, secretion of a diverse array of molecules, and transuterine migration to interact with the uterine surface. Up to date, the intricate transcriptome and proteome changes of the embryo underlying these events have not been critically studied in horses.

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Section: Discussionmentioning

confidence: 99%

Dynamic regulation of the transcriptome and proteome of the equine embryo during maternal recognition of pregnancy

et al. 2022

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“…In domestic animals, some miRNA variants are associated with economically important traits. For example, g.17104G > A of miR-208b is associated with the proportions of type I and IIb fibre numbers and drip loss in Berkshire, Landrace, and Yorkshire pigs [ 51 ], a G > A variant of miR-22 is related to meat colour parameters in Suhuai pigs [ 52 ], g.–113C > A of miR-9 is related to litter size in Markhoz goats [ 53 ], and g.–13G > A and g.–24T > G of miR-27a-3p are associated with litter size in Hu sheep [ 54 ]. In the present study, we identified a novel variant (g.–703indelT) of miR-423, an inhibitor of sow GC apoptosis, that is markedly associated with the NSB trait.…”

Section: Discussionmentioning

confidence: 99%

miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis

Li,

Zhang,

Wang

et al. 2023

J Animal Sci Biotechnol

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Background Atresia and degeneration, a follicular developmental fate that reduces female fertility and is triggered by granulosa cell (GC) apoptosis, have been induced by dozens of miRNAs. Here, we report a miRNA, miR-423, that inhibits the initiation of follicular atresia (FA), and early apoptosis of GCs. Results We showed that miR-423 was down-regulated during sow FA, and its levels in follicles were negatively correlated with the GC density and the P4/E2 ratio in the follicular fluid in vivo. The in vitro gain-of-function experiments revealed that miR-423 suppresses cell apoptosis, especially early apoptosis in GCs. Mechanically speaking, the miR-423 targets and interacts with the 3'-UTR of the porcine SMAD7 gene, which encodes an apoptosis-inducing factor in GCs, and represses its expression and pro-apoptotic function. Interestingly, FA and the GC apoptosis-related lncRNA NORHA was demonstrated as a ceRNA of miR-423. Additionally, we showed that a single base deletion/insertion in the miR-423 promoter is significantly associated with the number of stillbirths (NSB) trait of sows. Conclusion These results demonstrate that miR-423 is a small molecule for inhibiting FA initiation and GC early apoptosis, suggesting that treating with miR-423 may be a novel approach for inhibiting FA initiation and improving female fertility.

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“…However, only a few candidate miRNAs for female reproductive phenotypes, or single‐nucleotide variants (SNVs) significantly associated with female reproductive phenotypes, have been identified (Cho et al, 2021; Tyc et al, 2021). In domestic animals, only one SNV, g.‐113C>A, in the miR‐9 promoter was significantly associated with female fertility traits in goats (Mahmoudi et al, 2020), but its role in tissues and cells of the hypothalamic–pituitary–gonadal axis is unclear.…”

Section: Figurementioning

confidence: 99%

Two single nucleotide variants in the miR‐23a promoter affect granulosa cell apoptosis

Wang

et al. 2022

Animal Genetics

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microRNAs (miRNAs) are well known to be important in mammalian female fertility. However, the genetic regulation of miRNAs associated with female fertility remains largely unknown. Here, we report that two singlenucleotide variants (SNVs) in the miR-23a promoter strongly influence miR-23a transcription and function in granulosa cell (GC) apoptosis. Two novel SNVs, g.-283G>C and g.-271C>T, were detected in the porcine miR-23a promoter by pooled-DNA sequencing. Furthermore, SNVs in the promoter region influenced miR-23a transcription in porcine GCs by altering its promoter activity. Functionally, SNVs in the promoter strongly influenced miR-23a regulation of early apoptosis in porcine GCs cultured in vitro. In addition, a preliminary association analysis showed that the combined genotypes of the two SNVs, rather than a single SNV, were tentatively associated with sow fertility traits in a Large White population. Overall, our findings suggest that the SNVs g.-283G>C and g.-271C>T in the miR-23a promoter are causal variants affecting GC apoptosis and miR-23a may be a potential small-molecule nonhormonal drug for regulating female fertility.

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A novel variant in the promoter region of miR-9 gene strongly affects litter size in Markhoz goats

Cited by 6 publications

References 25 publications

Dynamic regulation of the transcriptome and proteome of the equine embryo during maternal recognition of pregnancy

Dynamic regulation of the transcriptome and proteome of the equine embryo during maternal recognition of pregnancy

miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis

Two single nucleotide variants in the miR‐23a promoter affect granulosa cell apoptosis

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