2007
DOI: 10.1007/s10735-007-9136-z
|View full text |Cite
|
Sign up to set email alerts
|

A novel use of TAT-EGFP to validate techniques to alter osteosarcoma cell surface glycosaminoglycan expression

Abstract: Several methods to alter cell surface glycosaminoglycan (GAG) expression have previously been described, including treatments with chlorate to reduce the addition of charged sulfate groups, xyloside compounds to displace GAGs from their core proteins, and GAG lyases, such as heparinase and chondroitinase, to release GAG fragments from the cell layer. While these methods are useful in identifying cellular mechanisms which are dependent on GAGs, they must be stringently validated to assess results in the appropr… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

0
15
0

Year Published

2007
2007
2015
2015

Publication Types

Select...
6

Relationship

1
5

Authors

Journals

citations
Cited by 6 publications
(15 citation statements)
references
References 82 publications
0
15
0
Order By: Relevance
“…Cell culture and dose-response assays MG-63 osteosarcoma cells (ATCC, Manassas, VA) and human mesenchymal stem cells (hMSCs, Cambrex Corporation, East Rutherford, NJ) were used as previously described (Kumarasuriyar et al, 2007). Cultures were seeded at 3,000 cells/cm 2 in 6-well plates under differentiating conditions until assay endpoint.…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…Cell culture and dose-response assays MG-63 osteosarcoma cells (ATCC, Manassas, VA) and human mesenchymal stem cells (hMSCs, Cambrex Corporation, East Rutherford, NJ) were used as previously described (Kumarasuriyar et al, 2007). Cultures were seeded at 3,000 cells/cm 2 in 6-well plates under differentiating conditions until assay endpoint.…”
Section: Methodsmentioning
confidence: 99%
“…DNA was extracted by lysing cells in dH 2 O and freeze-thawing four times before centrifugation. Alkaline phosphatase (ALP) activity was assessed as previously described (Kumarasuriyar et al, 2007). To measure calcium content, cell layers were lysed with 1% trichloroacetic acid and the supernatant mixed 1:10 with calcium arsenazo reagent (Pointe Scientific, Canton, MI).…”
Section: Cell-based Assaysmentioning
confidence: 99%
See 1 more Smart Citation
“…Sulfate moieties of GAG mimetics are required for the antiprion activity (40). Sodium chlorate, a competitive inhibitor of the cellular 3=-phosphoadenosine 5=-phosphosulfate, prevents both HS and CS sulfation (41)(42)(43) and also decreases PrP Sc accumulation in persistently infected cells (31,44). GAG sulfation also affects PrP Sc formation in in vitro assays and thus directly acts on PrP Sc amplification (45).…”
mentioning
confidence: 99%
“…Perhaps the use of a more naïve source, or more homogenous population of stem cells, such as those found in amniotic fluid (Kolambkar et al 2007), or following immuno-selection ), may prove more successful. One approach to overcome this is the transplantation of an acellular bioactive construct containing heparan (Kumarasuriyar et al 2007;Woodruff et al 2007) or chondroitin sulfate (Hui et al 2007), molecules that are thought to stimulate autologous stem cells within the host.…”
mentioning
confidence: 99%