A Novel Use of Alginate Hydrogel as Schwann Cell Matrix

Mosahebi, Afshin; Simon, Mary Alice; Wiberg, Mikael; Terenghi, Giorgio

doi:10.1089/107632701753213156

Cited by 101 publications

(62 citation statements)

References 43 publications

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“…Alginate is a naturally derived polymer, which has been wildly employed in dispensing-based biofabrication for various applications such as nerve tissue engineering [5,6] and bone tissue engineering [7,8], because of its good biocompatibility and ease gelation in the presence of calcium ions [5,9,10]. And calcium ions have been used to crosslink alginate in order to form hydrogel in dispensing-based biofabrication process.…”

Section: Introductionmentioning

confidence: 99%

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Cao

Chen

Schreyer

2012

ISRN Chemical Engineering

111

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One goal of biofabrication is to incorporate living cells into artificial scaffolds in order to repair damaged tissues or organs. Although there are many studies on various biofabrication techniques, the maintenance of cell viability during the biofabrication process and cell proliferation after the process is still a challenging issue. Construction of scaffolds using hydrogels composed of natural materials can avoid exposure of cells to harsh chemicals or temperature extremes but can still entail exposure to nonphysiological conditions, causing cell damage or even death. This paper presents an experimental investigation into the influence on Schwann cell survival and proliferation of calcium used for ionic crosslinking of alginate hydrogel during the biofabrication process. The experimental results obtained show the viability and proliferation capacity of cells, either suspended in cell culture medium or encapsulated in hydrogel, and vary with the calcium concentration and the time period of cells exposed to the calcium environment. The experimental results also show the alginate concentration and cell density, that have profound influence on cell survival and proliferation, and solution viscosity as well. This study suggests the incorporation of living cells in calcium-crosslinked hydrogel in the biofabrication process can be regulated for controlled cell survival and proliferation.

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Section: Introductionmentioning

confidence: 99%

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Cao

Chen

Schreyer

2012

ISRN Chemical Engineering

111

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“…Alginate has many applications in the food and pharmaceutical industries, and in medical research it has been used as a material for the sustained release of bovine serum albumin (BSA), fibroblast growth factor (FGF), immunoglobulin G (IgG), nerve growth factor (NGF), transforming growth factor (TGF-), TGF-, live cells, and more. [13][14][15] Alginate also has been used as scaffold material for tissue engineering applications, 16 has been used to encapsulate Schwann cells, 17 and most importantly to our research, has been shown to enhance elongation of amputated axons of infant rat spinal cords. [18][19][20] We have created a biodegradable fiber format capable of encapsulating a variety of biologically sensitive molecules and/or carrying drug-loaded microspheres or lipospheres.…”

Section: Introductionmentioning

confidence: 99%

Release of bovine serum albumin from a hydrogel‐cored biodegradable polymer fiber

Crow

Nelson

2006

Biopolymers

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We have developed a novel biodegradable, polymeric fiber construct that is coextruded using a wet-spinning process into a core-sheath format with a polysaccharide pre-hydrogel solution as the core fluid and poly(L-lactic acid) (PLLA) as the sheath. The biodegradable, biocompatible fibers were extruded from polymeric emulsions comprised of solutions of various molecular weights of PLLA dissolved in chloroform and containing dispersed, protein-free aqueous phases comprising up to 10% of the emulsion volume. Biologically sensitive agents can be loaded via a dispersed aqueous phase in the polymer, and/or directly into the polysaccharide. We show that this core-sheath fiber format will load a model protein that can be delivered for extended periods in vitro. Bovine serum albumin (BSA) was loaded into the fiber core as a model protein. We have shown that the greater the volume of the protein-free aqueous phase dispersed into the polymeric continuous-phase emulsion, the greater the total release of BSA encapsulated by a core gel comprised of 1% sodium alginate solution. We conclude this fiber format provides a promising vehicle for in vivo delivery of biological molecules. Its biocompatibility and biodegradability also allow for its use as a possible substrate for tissue engineering applications.

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“…[25][26][27] Alternatively, the nerve guide can be filled with SCs in a supporting adhesive matrix. [12][13][14]17,28 Therefore, the effect of fibronectin coating of TMC and CL (co)polymers on the behavior of HSCs was also investigated.…”

Section: Introductionmentioning

confidence: 99%

Adhesion and growth of human Schwann cells on trimethylene carbonate (co)polymers

Pêgo

Vleggeert-Lankamp

Deenen

et al. 2003

J Biomedical Materials Res

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Seeding of artificial nerve grafts with Schwann cells is a promising strategy for bridging large nerve defects. The aim of the present study was to evaluate the adhesion and growth of human Schwann cells (HSCs) on 1,3-trimethylene carbonate (TMC) and epsilon-caprolactone copolymers, with the final goal of using these materials in the development of an artificial nerve graft. The adhesion, proliferation, and morphology of HSCs on copolymers containing 10 and 82 mol % of TMC and on the parent homopolymers were investigated. HSCs adhered faster and in greater numbers on the copolymer with 82 mol % of TMC and on the TMC homopolymer compared with the other (co)polymers. On all polymer films, cell adhesion was lower than on gelatin (positive control). Despite differences in cell adhesion, cells displayed exponential growth on all tested surfaces, with similar growth rates. Cell numbers doubled approximately every 3 days on all substrates. When the polymer films were coated with fibronectin, no significant differences in cell adhesion and proliferation were observed between coated polymer surfaces and gelatin. The results indicate that all tested materials support the adhesion and proliferation of HSCs and can in principle be used for the preparation of flexible and slowly degrading nerve guides.

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A Novel Use of Alginate Hydrogel as Schwann Cell Matrix

Cited by 101 publications

References 43 publications

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Influence of Calcium Ions on Cell Survival and Proliferation in the Context of an Alginate Hydrogel

Release of bovine serum albumin from a hydrogel‐cored biodegradable polymer fiber

Adhesion and growth of human Schwann cells on trimethylene carbonate (co)polymers

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