A novel tyrosinase biosensor based on hydroxyapatite–chitosan nanocomposite for the detection of phenolic compounds

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

“…Electrochemical biosensor has been considered as the best choice for the in situ monitoring of active compounds (e.g., phenolic) by virtue of its high sensitivity, simple instrumentation, low production cost and promising response speed (Lu et al, 2010). Excellent membrane forming ability of chitosan nanoparticles and their small response time and high sensitivity and stability (due to their high surface to volume ratio), low cost and hydrophilicity making them suitable for biosensor applications that are mostly concerned with working of enzymes for detection mechanisms (Nakorn, 2008).…”

The Investigation of Electrochemistry Behaviors of Tyrosinase Based on Directly Electrodeposited Graphene on Choline–Gold Nanoparticals

“…Many efforts have been devoted to develop a simple and effective analytical method for the determination of phenols. Electrochemical biosensor has been considered as the best choice for in situ monitoring of phenolic compounds by virtue of its high sensitivity, simple instrumentation, low production cost and promising response speed [5], However, determination of phenols through the direct electrochemical is suffered from a number of drawbacks due to the high overvoltage, a high anodic potential needs to be applied, which open up the detection system for interfering reactions [6]. And, the high applied voltage is also followed by an increase of background current and noise level.…”

“…Electrochemical biosensors based on Tyr are simple and convenient tools for phenol assays due to their high sensitivity, effectiveness and simplicity [12]. Many nanomaterials, such as carbon-coated nickel nanoparticles [13], polyaniline (PANI) [14], hydroxyapatite [15], Fe3O4 nanoparticles [4], ZnO nanorod microarrays [16] and sonogel-carbon [17] have been used to construct Tyr biosensor for the detection of catechol (CA). It shows advantages of good reliability, fast response, inexpensive instrument, low energy consumption, simple operation, time saving and high sensitivity [18].…”

Electroanalysis of urinary l-dopa using tyrosinase immobilized on gold nanoelectrode ensembles

“…Electrochemical biosensors based on tyrosinase (Tyr) or polyphenol oxidase enzymes are con-sidered as an alternative to the conventional techniques due to their simplified sample treatment, and the possibility of portable, economic, fast, and sensitive analysis [5][6][7]. Several research groups have investigated Tyr-based bio-sensors for the detection of phenols [8][9][10]. Tyrosinasecatalysed oxidation of tyrosine and other monohydric phenols involves o-hydroxylation followed by oxidation of the resulting dihydric phenol to the corresponding o-qui-none in a single step without the release of the dihydric phenol intermediate.…”