A novel tumor‐related protein, C7orf24, identified by proteome differential display of bladder urothelial carcinoma

Kageyama, Susumu; Iwaki, Hideaki; Inoue, Hirokazu; Isono, Takahiro; Yuasa, Takeshi; Nogawa, Masaki; Maekawa, Taira; Ueda, Masamichi; Kajita, Yoichiro; Ogawa, Osamu; Toguchida, Junya; Tokura, Y.

doi:10.1002/prca.200600468

Cited by 49 publications

(68 citation statements)

References 11 publications

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“…The sequence of siRNA used in this study has shown efficient gene silencing of C7orf24 in other cell lines, such as A549 cells. 1 In anti-C7orf24 siRNA-transfected cells, the C7orf24 mRNA level was decreased approximately 90% in comparison with untreated cells (non-treatment) (Figure 1). On the other hand, an anti-luciferase siRNA (control siRNA) had almost no effect on the knockdown of C7orf24 like untreated cells.…”

Section: Resultsmentioning

confidence: 99%

“…In recent years, owing to progress in genome-and proteome-wide analyses, novel attractive target molecules have been identified in various types of cancer. Among these molecules, chromosome 7 open reading frame 24 (C7orf24), which was identified by proteome analysis of bladder cancer specimens, 1 is a promising target molecule for the following reasons: C7orf24 is upregulated in various types of cancer, such as prostate cancer, 2 breast cancer 3 and osteosarcoma, 4 while it is expressed at a low level in normal tissues. 1 Furthermore, overexpression of C7orf24 accelerated cellular growth in NIH3T3 cells, 1 and C7orf24 was also involved in geranyl geraniol-induced apoptosis.…”

Section: Introductionmentioning

confidence: 99%

“…Among these molecules, chromosome 7 open reading frame 24 (C7orf24), which was identified by proteome analysis of bladder cancer specimens, 1 is a promising target molecule for the following reasons: C7orf24 is upregulated in various types of cancer, such as prostate cancer, 2 breast cancer 3 and osteosarcoma, 4 while it is expressed at a low level in normal tissues. 1 Furthermore, overexpression of C7orf24 accelerated cellular growth in NIH3T3 cells, 1 and C7orf24 was also involved in geranyl geraniol-induced apoptosis. 5 Therefore, C7orf24 is a key molecule controlling tumor survival, and prevention of C7orf24 functionality is a promising approach to develop new cancer therapies.…”

Section: Introductionmentioning

confidence: 99%

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Prevention of tumor growth by needle-free jet injection of anti-C7orf24 siRNA

et al. 2012

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Chromosome 7 open reading frame 24 (C7orf24), which was identified by proteome analysis, is upregulated in various types of cancer and is associated with cellular proliferation. However, in vivo antitumor effect by knockdown of C7orf24 has not been clarified. In this study, we investigated that the antitumor effect of anti-C7orf24 small interfering RNA (siRNA) administered by needle-free jet injection (JI) on lung cancer-bearing mice. Transfection of anti-C7orf24 siRNA induced cytotoxicity in cultured human lung cancer cells through specific knockdown of C7orf24. Furthermore, JI could effectively deliver anti-C7orf24 siRNA to tumor tissues, and as a result tumor growth was significantly inhibited. Immunohistochemical analysis revealed that C7orf24 levels were significantly reduced within tumor tissues collected from anti-C7orf24 siRNA-administered mice, indicating that the knockdown of C7orf24 induced cytotoxicity in tumor tissue. In conclusion, these data show for the first time that knockdown of C7orf24 prevents tumor growth in vivo following JI-mediated the siRNA delivery.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevention of tumor growth by needle-free jet injection of anti-C7orf24 siRNA

et al. 2012

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“…In several previous studies, differential expression of C7orf24 (GGCT) was noted in tumor tissues (Gromov et al 2010;Kageyama et al 2007;Uejima et al 2011;Xu et al 2007;Zhang et al 2006), and it has been proposed that it may be a cancer biomarker. However, little is known about the cellular distribution of GGCT in normal human tissues or in human tumors.…”

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confidence: 98%

Widespread Expression of γ-Glutamyl Cyclotransferase Suggests It Is Not a General Tumor Marker

Amano

Eishi

Yamada

et al. 2011

J Histochem Cytochem.

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Summary γ-Glutamyl cyclotransferase (GGCT) contributes to the γ-glutamyl cycle that regulates glutathione metabolism. Although GGCT has been implicated in several studies as a possible cancer marker, little is known about its distribution in cells and tissues. The authors investigated GGCT expression in normal tissues and tumors using Western blots and immunohistochemistry with a novel anti-GGCT monoclonal antibody. GGCT was detected in most organs and was mainly found in epithelial cells. Although the intracellular distribution was mainly cytoplasmic, in some situations, nuclear staining was strong. A significant increase in the expression of GGCT was found in tumors of the lung, esophagus, stomach, bile duct, and uterine cervix. In contrast, there was a significant decrease in expression in renal and urothelial tumors. These results suggest that GGCT may be a biomarker of tumors in a limited range of organs. (J Histochem Cytochem 60:76-86, 2012)

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“…12) Silencing of GGCT using small interfering RNA (siRNA) inhibited cancer cell proliferation in an in vitro study. 1,13) Hama et al reported the antitumor effect of anti-GGCT siRNA on lung cancer xenograft mice. 19) He's group has demonstrated that systemic administration of PEGylated hyaluronic acid-modified liposomal at siRNA could suppress breast tumor growth.…”

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confidence: 99%