A Novel Trehalose Synthase for the Production of Trehalose and Trehalulose

Agarwal, Neera; Singh, Sudhir P.

doi:10.1128/spectrum.01333-21

Cited by 12 publications

(10 citation statements)

References 62 publications

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“…Currently available approaches for trehalose production are based on enzymatic transformations rather than extraction from biomass or in vitro chemical synthesis (Agarwal & Singh, 2021). In one of the attempts to commercially produce trehalose, a number of groups reported its production with glucose‐1‐phosphate and d ‐glucose catalyzed by TreP (Figure 2b) (Chaen et al., 1999).…”

Section: Natural and Industrial Trehalose Production Methodsmentioning

confidence: 99%

“…The fourth pathway of trehalose production relies on trehalose synthase (TreS), which catalyzes an intramolecular rearrangement of maltose by converting the α(1–4) glycosidic link of maltose to α(1–1) of trehalose (Figure 2d) (Agarwal & Singh, 2021; T. K. Kim et al., 2010; Nakada et al., 1995; Nishimoto, Nakada, et al., 1996; Saito et al., 1998; Tsusaki et al., 1996; J. Wang et al., 2017; R. Zhang et al., 2011). First isolated from Pimelobacter , orthologs of TreS have also been identified in other bacteria (Nishimoto, Nakano, et al., 1996).…”

Section: Natural and Industrial Trehalose Production Methodsmentioning

confidence: 99%

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Trehalose and its applications in the food industry

Chen

Tapia

Goddard

et al. 2022

Comp Rev Food Sci Food Safe

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Trehalose is a nonreducing disaccharide composed of two glucose molecules linked by α, α-1,1-glycosidic bond. It is present in a wide variety of organisms, including bacteria, fungi, insects, plants, and invertebrate animals. Trehalose has distinct physical and chemical properties that have been investigated for their biological importance in a range of prokaryotic and eukaryotic species. Emerging research on trehalose has identified untapped opportunities for its application in the food, medical, pharmaceutical, and cosmetics industries. This review summarizes the chemical and biological properties of trehalose, its occurrence and metabolism in living organisms, its protective role in molecule stabilization, and natural and commercial production methods. Utilization of trehalose in the food industry, in particular how it stabilizes protein, fat, carbohydrate, and volatile compounds, is also discussed in depth. Challenges and opportunities of its application in specific applications (e.g., diagnostics, bioprocessing, ingredient technology) are described. We conclude with a discussion on the potential of leveraging the unique molecular properties of trehalose in molecular stabilization for improving the safety, quality, and sustainability of our food systems.

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Section: Natural and Industrial Trehalose Production Methodsmentioning

confidence: 99%

Section: Natural and Industrial Trehalose Production Methodsmentioning

confidence: 99%

Trehalose and its applications in the food industry

Chen

Tapia

Goddard

et al. 2022

Comp Rev Food Sci Food Safe

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“…Domain A (27–125, 202–353 and 379–477) located at the N-terminus is a catalytic module containing the TIM barrel structure. The proposed key amino acid residues are consisted of H128/D225/E267/H334/D335 (Agarwal and Singh 2021 ) (Fig. 1 B).…”

Section: Resultsmentioning

confidence: 99%

Characterization of cold-active trehalose synthase from Pseudarthrobacter sp. for trehalose bioproduction

Trakarnpaiboon,

Bunterngsook,

Lekakarn

et al. 2023

Bioresour. Bioprocess.

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Trehalose is a functional sugar that has numerous applications in food, cosmetic, and pharmaceutical products. Production of trehalose from maltose via a single-step enzymatic catalysis using trehalose synthase (TreS) is a promising method compared with the conventional two-step process due to its simplicity with lower formation of byproducts. In this study, a cold-active trehalose synthase (PaTreS) from Pseudarthrobacter sp. TBRC 2005 was heterologously expressed and characterized. PaTreS showed the maximum activity at 20 °C and maintained 87% and 59% of its activity at 10 °C and 4 °C, respectively. The enzyme had remarkable stability over a board pH range of 7.0–9.0 with the highest activity at pH 7.0. The activity was enhanced by divalent metal ions (Mg2+, Mn2+ and Ca2+). Conversion of high-concentration maltose syrup (100–300 g/L) using PaTreS yielded 71.7–225.5 g/L trehalose, with 4.5–16.4 g/L glucose as a byproduct within 16 h. The work demonstrated the potential of PaTreS as a promising biocatalyst for the development of low-temperature trehalose production, with the advantages of reduced risk of microbial contamination with low generation of byproduct. Graphical abstract

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“…The production of trehalose can be achieved either by chemically or biologically. The chemical route is performed through the reaction between 2,3,4,5-tetra-O-acetyl-D-glucose and 3,4,6-tri-O-acetyl-1,2anhydro-D-glucose [9]. Meanwhile, the biological route of trehalose production is undertaken by utilizing enzymes via three different biosynthetic pathways [9].…”

Section: Introductionmentioning

confidence: 99%

“…The chemical route is performed through the reaction between 2,3,4,5-tetra-O-acetyl-D-glucose and 3,4,6-tri-O-acetyl-1,2anhydro-D-glucose [9]. Meanwhile, the biological route of trehalose production is undertaken by utilizing enzymes via three different biosynthetic pathways [9]. The first pathway uses two enzymes, namely trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase.…”

Section: Introductionmentioning

confidence: 99%

DNA marker utilization for the sustainable production of trehalose

Trinugroho,

Asadi,

Hidayat

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Trehalose is a type of sugar that is known by its stability and resilience towards acid and low temperature. Furthermore, trehalose has numerous health benefits and has been used by several industries, including food, cosmetics, and pharmaceuticals. Even though trehalose could be easily produced using trehalose synthase (TreS) enzyme, a sustainable production of trehalose is still a problem. Our work aims to develop an approach to identify a novel trehalose synthase enzyme from various organisms, especially thermophilic bacteria, by implementing a deoxyribonucleic acid (DNA) marker technique. We first collected protein and DNA sequences from public biological databases and subsequently conducted sequence analysis. We then designed degenerate primers based on the conserved regions identified from the sequence analysis. The designed primers were subjected to primer characterization using Oligo Calc software. The primers were further validated via in-silico PCR amplification. In general, our designed primers possess the properties to work optimally. In addition, agarose gel electrophoresis that the primers successfully amplified nucleotides encoding TreS enzyme from all samples. Our findings may serve as a basis to discover the TreS enzyme variants which possess superior attributes, allowing the sustainable production of trehalose.

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A Novel Trehalose Synthase for the Production of Trehalose and Trehalulose

Abstract: Trehalose is a rare sugar of high importance in biological research, with its property to stabilize cell membrane and proteins and protect the organism from drought. It is instrumental in the cryopreservation of human cells, e.g., sperm and blood stem cells.

Cited by 12 publications

References 62 publications

Trehalose and its applications in the food industry

Trehalose and its applications in the food industry

Characterization of cold-active trehalose synthase from Pseudarthrobacter sp. for trehalose bioproduction

DNA marker utilization for the sustainable production of trehalose

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