A novel treatment option for photoaged skin

Knott, Anja; Koop, Urte; Mielke, Heiko; Reuschlein, Katja; Peters, Nils; Muhr, Gesa-Meike; Lenz, Holger; Wensorra, Ursula; Jaspers, Sören; Kolbe, Ludger; Raschke, T.; Stäb, Franz; Wenck, Horst; Gallinat, Stefan

doi:10.1111/j.1473-2165.2008.00356.x

Cited by 23 publications

(17 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The integrity of the formed barrier could be demonstrated by measuring the electrical resistance during culture. The electrical barrier reached 192 Ω*cm 2 , which is comparable to other in vitro models but below the value of human skin (Knott et al, 2008). The capacitance was investigated as a second electrical parameter.…”

Section: Resultsmentioning

confidence: 80%

A first vascularized skin equivalent for as an alternative to animal experimentation

Groeber

Engelhardt

Lange

et al. 2016

ALTEX

View full text Add to dashboard Cite

whereas for tissue-engineered skin implants, new vessels must be formed by angiogenesis, which delays graft integration (Young et al., 1996). Consequently, bio-engineered skin implants are more likely to be rejected. In addition, the cutaneous vasculature is crucial for several physiological and pathophysiological processes including the development of skin diseases, wound healing, metastasizing of malignant melanoma, tumor-angiogenesis, autoand alloimmune-phenomena, and the transdermal penetration of substances. Taken together, non-vascularized skin models are of limited value with regard to their ability to reflect the physiological conditions of a full organ. In the absence of a model that represents the physiological conditions of a full organ, there remains a scientific and medical need for animal models.To overcome these limitations, endothelial cells can be seeded into the dermal part of full-thickness skin equivalents, which results in the alignment of endothelial cells to vessel-like struc- IntroductionTissue-engineered, three-dimensional skin equivalents are capable of mimicking key anatomical, metabolic, cellular and functional aspects of native human skin. Thus, they can be employed as wound coverage for large skin defects or as in vitro test systems instead of animal models in basic research (Groeber et al., 2011). Generally, two types of tissue-engineered skin models are available, being representatives of either the epidermis alone (reconstructed human epidermis) or the dermal and epidermal layer (full-thickness skin equivalents) (De Wever et al., 2013). In spite of recent progress, the use of current skin equivalents for medical purposes and as test systems remains limited owing to the lack of a functional vasculature.In skin transplantation, an existing vasculature supports a rapid anastomosis of donor skin to the host's vasculature (inosculation), Research SummaryTissue-engineered skin equivalents mimic key aspects of the human skin and can thus be employed as wound coverage for large skin defects or as in vitro test systems as an alternative to animal models. However, current skin equivalents lack a functional vasculature, limiting clinical and research applications. This study demonstrates the generation of a vascularized skin equivalent with a perfused vascular network by combining a biological vascularized scaffold (BioVaSc) based on a decellularized segment of porcine jejunum and a tailored bioreactor system. The BioVaSc was seeded with human fibroblasts, keratinocytes, and human microvascular endothelial cells. After 14 days at the air-liquid interface, hematoxylin & eosin and immunohistological staining revealed a specific histological architecture representative of the human dermis and epidermis, including a papillary-like architecture at the dermal-epidermal-junction. The formation of the skin barrier was measured non-destructively using impedance spectroscopy. Additionally, endothelial cells lined the walls of the formed vessels that could be perfused with a physiological volume flow...

show abstract

Section: Resultsmentioning

confidence: 80%

A first vascularized skin equivalent for as an alternative to animal experimentation

Groeber

Engelhardt

Lange

et al. 2016

ALTEX

View full text Add to dashboard Cite

show abstract

“…One forearm is treated as described above; the colateral forearm is left untreated. To assess DNA damage by single-cell gel electrophoresis (Comet assay), epidermal keratinocytes are isolated from suction blister epidermis and embedded in low gelling agarose gels [47].…”

Section: Methodsmentioning

confidence: 99%

“…At the baseline visit and after 1, 2, and 4 weeks of regular use, skin viscoelasticity is determined using a cutometer. As an additional means of control for volunteer compliance, skin moisture is examined using a corneometer [47,48].…”

Section: Methodsmentioning

confidence: 99%

“…At the baseline visit and after 4 weeks of treatment, facial wrinkles in the crow's feet area are evaluated by means of in vivo topometry using phase shift rapid in vivo measuring of human skin phase induction PRIMOS. The PRIMOS system represents an established and widely used method to quantify effects on skin wrinkles [47].…”

Section: Methodsmentioning

confidence: 99%

“…Ten to 20 h after application of test samples, measurements are performed. The determination is carried out by image analysis of D-Squame ® sheets [47].…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Herbal Photoprotective Formulations and their Evaluation

Chanchal¹,

Saraf²

2009

TONPJ

View full text Add to dashboard Cite

Photochemoprevention has become an important armamentarium in the fight against ultraviolet radiation induced damage to the skin. UV irradiation to skin results in erythema, edema, sunburn cells, hyperplasia, immunosuppression, photoaging and photocarcinogenesis. Various synthetic agents have been used as photoprotectives but they have limited use because of their potential toxicity in humans and their ability to interfere only in selected pathways of multistage process of carcinogenesis. Several botanical compounds have been shown to be antimutagenic, anticarcinogenic and nontoxic and have ability to exert striking inhibitory effects on a plethora of cellular events at various stages of carcinogenesis. Since multiple pathways are involved in photocarcinogenesis so mixture of several botanical antioxidants working through various mechanisms, in conjunction with the use of sunscreens could also be an effective approach for reducing photoaging and skin cancer in humans. The performance of sunscreen substances could be improved by modification of their chemical, physical and technological properties or by the use of novel carriers like liposomes, nanoparticles, phytosomes, transferosomes, nanospheres etc. The application of novel approaches can also improve its efficacy regarding continuous action of herbs on the human body. This review will focus on list of herbs, formulations and evaluation parameters of photoprotective herbs.

show abstract