A novel transducible chimeric phage from Escherichia coli O157:H7 Sakai strain encoding Stx1 production

Sváb, Domonkos; Bálint, Balázs; Maróti, Gergely; Tóth, István

doi:10.1016/j.meegid.2014.10.019

Cited by 8 publications

(2 citation statements)

References 28 publications

(40 reference statements)

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“…This relationship seems to be irrespective of stx type, because the closest relationship was to ɸ191 and p13374, 2 stx2 -converting phages of STEC associated with outbreaks of enteroaggregative hemorrhagic E coli . Given the overall similarity of these phages apart from the stx operon, the findings implicate a recombination event leading to similar phages with differing toxin operons, a phenomenon that has been observed in STEC phages [ 14 ]. A weaker phylogenetic relationship was observed between ɸSs-VASD and the S flexneri phage POC-J13, and the S sonnei -converting phage 75-02.…”

Section: Discussionmentioning

confidence: 99%

A Novel Shiga Toxin 1a-Converting Bacteriophage of Shigella sonnei With Close Relationship to Shiga Toxin 2-Converting Pages of Escherichia coli

Carter

Fierer

Chiu

et al. 2016

Open Forum Infectious Diseases

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In recent studies, strains of non-dysenteriae 1 Shigella (NDS) expressing Shiga toxin have been reported. In this study, we report a novel stx1a-converting bacteriophage of Shigella sonnei associated with travel to Mexico. Phylogenetic comparison between this and other stx-converting phages suggests that toxigenic NDS strains have arisen through separate horizontal transfer events from toxigenic Escherichia coli.

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Section: Discussionmentioning

confidence: 99%

A Novel Shiga Toxin 1a-Converting Bacteriophage of Shigella sonnei With Close Relationship to Shiga Toxin 2-Converting Pages of Escherichia coli

Carter

Fierer

Chiu

et al. 2016

Open Forum Infectious Diseases

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“…The genomic data of SD1 strains seem to exclude the possibility of the phage being transduced from them to STEC strains, albeit in the case of the prototypic enterohemorrhagic E. coli (EHEC) O157:H7 Sakai strain, the Stx1 gene is harbored on a defective prophage, which in turn can be mobilized through recombination with the Stx2-harboring inducible prophage (Asadulghani et al, 2009;Sváb et al, 2015). Such a recombination event could also have occurred between SD1 Stx1-carrying prophage and a more complete lambdoid phage.…”

Section: Discussionmentioning

confidence: 99%

Genome Analysis of a Historical Shigella dysenteriae Serotype 1 Strain Carrying a Conserved Stx Prophage Region

et al. 2021

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Shigella dysenteriae are significant agents of bacillary dysentery, accounting for a considerable number of illnesses with high morbidity worldwide. The Shiga toxin (Stx) encoded by a defective prophage is the key virulence factor of S. dysenteriae type 1 (SD1) strains. Here we present the full genome sequence of an SD1 strain HNCMB 20080 isolated in 1954, compare it to other sequenced SD1 genomes, and assess the diversity of Stx-prophages harbored by previously sequenced SD1 strains. The genome of HNCMB 20080 consists of a chromosome sized 4,393,622 bp containing 5,183 CDSs, as well as two small plasmids. Comparative genomic analysis revealed a high degree of uniformity among SD1 genomes, including the structure of Stx prophage regions, which we found to form two subgroups termed PT-I and PT-II. All PT-I strains are members of the sequence type (ST) 146 or ST260, while the only PT-II harboring strain, Sd1617 proved to be ST untypeable. In accordance with data from previous reports, the Stx1 prophage could not be induced from HNCMB 20080. Our cumulative data do not support the notion that stx-harboring phages in STEC are derived from historical SD1 isolates.

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Cholera Dynamics and the Emergence of Pandemic Vibrio cholerae

Balasubramanian

López‐Pérez²,

Almagro‐Moreno³

2023

Advances in Experimental Medicine and Biology

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A novel transducible chimeric phage from Escherichia coli O157:H7 Sakai strain encoding Stx1 production

Cited by 8 publications

References 28 publications

A Novel Shiga Toxin 1a-Converting Bacteriophage of Shigella sonnei With Close Relationship to Shiga Toxin 2-Converting Pages of Escherichia coli

A Novel Shiga Toxin 1a-Converting Bacteriophage of Shigella sonnei With Close Relationship to Shiga Toxin 2-Converting Pages of Escherichia coli

Genome Analysis of a Historical Shigella dysenteriae Serotype 1 Strain Carrying a Conserved Stx Prophage Region

Cholera Dynamics and the Emergence of Pandemic Vibrio cholerae

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