A novel transcriptional repressor complex <scp>MYB22</scp>–<scp>TOPLESS</scp>–<scp>HDAC1</scp> promotes rice resistance to brown planthopper by repressing <i>F3′H</i> expression

Sun, Bo; Shen, Yanjie; Su, Chen; Shi, Zhenying; Li, Haichao; Miao, Xuexia

doi:10.1111/nph.18958

Cited by 9 publications

(5 citation statements)

References 71 publications

(115 reference statements)

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“…For example, OsMYB30 (an R2R3 MYB TF) induces the expression of phenylalanine ammonia-lyase (PAL) enzymes, thereby improving BPH resistance in rice ( He et al., 2020 ). OsMYB22 promotes rice resistance by affecting flavonoid biosynthesis ( Sun et al., 2023 ). The bHLH protein MYC2 is involved in JA-mediated insect resistance ( Schweizer et al., 2013 ; Xu et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Combined miRNA and mRNA sequencing reveals the defensive strategies of resistant YHY15 rice against differentially virulent brown planthoppers

Yu,

Geng,

Xue

et al. 2024

Front. Plant Sci.

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IntroductionThe brown planthopper (BPH) poses a significant threat to rice production in Asia. The use of resistant rice varieties has been effective in managing this pest. However, the adaptability of BPH to resistant rice varieties has led to the emergence of virulent populations, such as biotype Y BPH. YHY15 rice, which carries the BPH resistance gene Bph15, exhibits notable resistance to biotype 1 BPH but is susceptible to biotype Y BPH. Limited information exists regarding how resistant rice plants defend against BPH populations with varying levels of virulence.MethodsIn this study, we integrated miRNA and mRNA expression profiling analyses to study the differential responses of YHY15 rice to both avirulent (biotype 1) and virulent (biotype Y) BPH.ResultsYHY15 rice demonstrated a rapid response to biotype Y BPH infestation, with significant transcriptional changes occurring within 6 hours. The biotype Y-responsive genes were notably enriched in photosynthetic processes. Accordingly, biotype Y BPH infestation induced more intense transcriptional responses, affecting miRNA expression, defenserelated metabolic pathways, phytohormone signaling, and multiple transcription factors. Additionally, callose deposition was enhanced in biotype Y BPH-infested rice seedlings.DiscussionThese findings provide comprehensive insights into the defense mechanisms of resistant rice plants against virulent BPH, and may potentially guide the development of insect-resistant rice varieties.

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Section: Discussionmentioning

confidence: 99%

Combined miRNA and mRNA sequencing reveals the defensive strategies of resistant YHY15 rice against differentially virulent brown planthoppers

Yu,

Geng,

Xue

et al. 2024

Front. Plant Sci.

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“…Conventionally, the EAR motifs harbored by repressive TFs are responsible for inhibiting gene expression in various plants (Kagale & Rozwadowski, 2011; Ni et al., 2023; Plant et al., 2021). EAR motif‐containing transcriptional repressors of MYB TFs can recruit their co‐repressors of TPL (Sun et al., 2023). For example, AtMYB44, which contains an EAR motif, interacts with TPR co‐repressors to suppress PP2C gene expression in Arabidopsis (Nguyen & Cheong, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…(Hu et al., 2020). For the MYB TFs, the MYB73/TPL/HDA19‐miR159‐CKX6 module influences petal size in Rosa hybrida (Jing et al., 2023), and the MYB22‐TOPLESS‐HDAC1 transcriptional repressor complex improves resistance to brown planthopper in rice ( Oryza sativa ) (Sun et al., 2023). The MYB75/HAT1/TPL transcriptional repressor complex influences anthocyanin biosynthesis through histone deacetylation of anthocyanin late biosynthetic genes in Arabidopsis (Zheng, Tan, et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

The MdMYB44‐MdTPR1 repressive complex inhibits MdCCD4 and MdCYP97A3 expression through histone deacetylation to regulate carotenoid biosynthesis in apple

Huang,

Li,

Jia

et al. 2024

The Plant Journal

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SUMMARYCarotenoids are photosynthetic pigments and antioxidants that contribute to different plant colors. However, the involvement of TOPLESS (TPL/TPR)‐mediated histone deacetylation in the modulation of carotenoid biosynthesis through ethylene‐responsive element‐binding factor‐associated amphiphilic repression (EAR)‐containing transcription factors (TFs) in apple (Malus domestica Borkh.) is poorly understood. MdMYB44 is a transcriptional repressor that contains an EAR repression motif. In the present study, we used functional analyses and molecular assays to elucidate the molecular mechanisms through which MdMYB44‐MdTPR1‐mediated histone deacetylation influences carotenoid biosynthesis in apples. We identified two carotenoid biosynthetic genes, MdCCD4 and MdCYP97A3, that were confirmed to be involved in MdMYB44‐mediated carotenoid biosynthesis. MdMYB44 enhanced β‐branch carotenoid biosynthesis by repressing MdCCD4 expression, whereas MdMYB44 suppressed lutein level by repressing MdCYP97A3 expression. Moreover, MdMYB44 partially influences carotenoid biosynthesis by interacting with the co‐repressor TPR1 through the EAR motif to inhibit MdCCD4 and MdCYP97A3 expression via histone deacetylation. Our findings indicate that the MdTPR1‐MdMYB44 repressive cascade regulates carotenoid biosynthesis, providing profound insights into the molecular basis of histone deacetylation‐mediated carotenoid biosynthesis in plants. These results also provide evidence that the EAR‐harboring TF/TPL repressive complex plays a universal role in histone deacetylation‐mediated inhibition of gene expression in various plants.

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“…OsWRKY53 enhances rice resistance to BPH by increasing H 2 O 2 levels and reducing the ethylene synthesis [109]. MYB22 and MYB30, R2R3-type MYB TFs, positively regulate rice resistance to BPH [110,111]. The EAR motif of MYB22 forms an MYB22-TOPLESS-HDAC1 complex, which negatively regulates the transcription of the F3'H (flavanone 3 -hydroxylase) gene, thereby enhancing rice resistance to BPH [110].…”

Section: Transcription Factorsmentioning

confidence: 99%

“…MYB22 and MYB30, R2R3-type MYB TFs, positively regulate rice resistance to BPH [110,111]. The EAR motif of MYB22 forms an MYB22-TOPLESS-HDAC1 complex, which negatively regulates the transcription of the F3'H (flavanone 3 -hydroxylase) gene, thereby enhancing rice resistance to BPH [110]. OsMYB30 mediates rice resistance to BPH by regulating the expression of OsPAL6 and OsPAL8 genes [111].…”

Section: Transcription Factorsmentioning

confidence: 99%

Recent Advances in Molecular Mechanism and Breeding Utilization of Brown Planthopper Resistance Genes in Rice: An Integrated Review

Yan

TongPing

Huang

et al. 2023

IJMS

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Over half of the world’s population relies on rice as their staple food. The brown planthopper (Nilaparvata lugens Stål, BPH) is a significant insect pest that leads to global reductions in rice yields. Breeding rice varieties that are resistant to BPH has been acknowledged as the most cost-effective and efficient strategy to mitigate BPH infestation. Consequently, the exploration of BPH-resistant genes in rice and the development of resistant rice varieties have become focal points of interest and research for breeders. In this review, we summarized the latest advancements in the localization, cloning, molecular mechanisms, and breeding of BPH-resistant rice. Currently, a total of 70 BPH-resistant gene loci have been identified in rice, 64 out of 70 genes/QTLs were mapped on chromosomes 1, 2, 3, 4, 6, 8, 10, 11, and 12, respectively, with 17 of them successfully cloned. These genes primarily encode five types of proteins: lectin receptor kinase (LecRK), coiled-coil-nucleotide-binding-leucine-rich repeat (CC-NB-LRR), B3-DNA binding domain, leucine-rich repeat domain (LRD), and short consensus repeat (SCR). Through mediating plant hormone signaling, calcium ion signaling, protein kinase cascade activation of cell proliferation, transcription factors, and miRNA signaling pathways, these genes induce the deposition of callose and cell wall thickening in rice tissues, ultimately leading to the inhibition of BPH feeding and the formation of resistance mechanisms against BPH damage. Furthermore, we discussed the applications of these resistance genes in the genetic improvement and breeding of rice. Functional studies of these insect-resistant genes and the elucidation of their network mechanisms establish a strong theoretical foundation for investigating the interaction between rice and BPH. Furthermore, they provide ample genetic resources and technical support for achieving sustainable BPH control and developing innovative insect resistance strategies.

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A novel transcriptional repressor complex MYB22–TOPLESS–HDAC1 promotes rice resistance to brown planthopper by repressing F3′H expression

Cited by 9 publications

References 71 publications

Combined miRNA and mRNA sequencing reveals the defensive strategies of resistant YHY15 rice against differentially virulent brown planthoppers

Combined miRNA and mRNA sequencing reveals the defensive strategies of resistant YHY15 rice against differentially virulent brown planthoppers

The MdMYB44‐MdTPR1 repressive complex inhibits MdCCD4 and MdCYP97A3 expression through histone deacetylation to regulate carotenoid biosynthesis in apple

Recent Advances in Molecular Mechanism and Breeding Utilization of Brown Planthopper Resistance Genes in Rice: An Integrated Review

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