A novel thylakoid-less isolate fills a billion-year gap in the evolution of Cyanobacteria

Rahmatpour, Nasim; Hauser, Duncan; Nelson, Jessica; Chen, Pa Yu; Villarreal, A Juan Carlos; Ho, Ming-Chih; Li, Fay‐Wei

doi:10.1016/j.cub.2021.04.042

Cited by 37 publications

(61 citation statements)

References 78 publications

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“…Gloeobacter morelensis is the sister lineage of G. violaceus PCC 7421 in the phylogenomic tree. The topology of the tree is similar to the tree obtained by Rahmatpour et al (2021) .…”

Section: Resultsmentioning

confidence: 67%

“…However, all sequences from all Gloeobacter strains, including the identical ones, were kept in the data set. An additional D1 from the metagenome-assembled genome of A. vandensis ( Grettenberger et al 2020 ) and the three amino acid sequences from the genome of A. panamensis were later added to the data set ( Rahmatpour et al 2021 ). Alignments were produced using Clustal Omega ( Sievers et al 2011 ) using ten combined guided-tree and HMM iterations.…”

Section: Methodsmentioning

confidence: 99%

“…However, although the genome content of Gloeobacterales appeared to show a slightly decreased number of identifiable accessory photosystem subunits ( Nakamura et al 2003 ; Saw et al 2013 ; Rahmatpour et al 2021 ), measurements of oxygen evolution activity in Gloeobacter violaceus PCC 7421 revealed no functional differences to model organisms ( Koyama et al 2008 ). The genome of Gloeobacter kilaueensis JS1, on the other hand, revealed an additional Photosystem II subunit with atypical characteristics previously undetected in the closely related G. violaceus genome.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, a novel strain closely related to A. vandensis that also lacks thylakoids was isolated from a hornwort and named Anthocerotibacter panamensis . Its complete genome was reported recently ( Rahmatpour et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Complete Genome Sequencing of a NovelGloeobacterSpecies from a Waterfall Cave in Mexico

Saw

Cardona

Montejano

2021

Genome Biology and Evolution

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Only two complete genomes of the cyanobacterial genus Gloeobacter from two very different regions of the world currently exist. Here we present the complete genome sequence of a third member of the genus isolated from a waterfall cave in Mexico. Analysis of the average nucleotide identities (ANI) between published Gloeobacter genomes revealed that the complete genome of this new member is only 92.7% similar to Gloeobacter violaceus and therefore we determined it to be a new species. We propose to name this new species Gloeobacter morelensis after the location in Mexico where it was isolated. The complete genome consists of one circular chromosome (4,921,229 bp), one linear plasmid (172,328 bp), and one circular plasmid (8,839 bp). Its genome is the largest of all completely sequenced genomes of Gloeobacter species. Pangenomic comparisons revealed that G. morelensis encodes 759 genes not shared with other Gloeobacter species. Despite being more closely related to G. violaceus, it features an extremely divergent psbA gene encoding an atypical D1 core subunit of Photosystem II previously only found within the genome of G. kilaueensis. In addition, we detected evidence of concerted evolution of psbA genes encoding identical D1 in all three Gloeobacter genomes, a characteristic that seems widespread in cyanobacteria and may therefore be traced back to their last common ancestor.

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“…Gloeobacter morelensis is the sister lineage of G. violaceus PCC 7421 in the phylogenomic tree. The topology of the tree is similar to the tree obtained by Rahmatpour et al (2021) .…”

Section: Resultsmentioning

confidence: 67%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Complete Genome Sequencing of a NovelGloeobacterSpecies from a Waterfall Cave in Mexico

Saw

Cardona

Montejano

2021

Genome Biology and Evolution

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“…1 b, c). Furthermore, our structure shows that OCP R interacts with the CpcG1 rod-linker, a conserved subunit assumed to be present in the ancestor of the Cyanobacteria 27 , contributing to the surface of interaction (Fig. 1 c, 3 b Extended Data Table 2 and 3).…”

Section: The Pbs-ocp Interactionmentioning

confidence: 86%

Structure of the Quenched Cyanobacterial OCP-Phycobilisome Complex

Domínguez-Martín

Sauer

Sutter

et al. 2021

Preprint

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Photoprotection is an essential mechanism in photosynthetic organisms to balance the harvesting of light energy against the risks of photodamage. In cyanobacteria, photoprotective non-photochemical quenching relies on the interaction between a photoreceptor, the Orange Carotenoid Protein (OCP), and the antenna, the phycobilisome (PBS). Here we report the first structure of the OCP-PBS complex at 2.7 Å overall resolution obtained by cryo-electron microscopy. The structure shows that the 6.2 MDa PBS is quenched by four 34 kDa OCP organized as two dimers. The complex also reveals that the structure of the active form of the OCP is drastically different than its resting, non-quenching form, with an ∼60 Å displacement of its regulatory domain. These results provide a high-resolution blueprint of the structural basis of the protective quenching of excess excitation energy that enables cyanobacteria to harvest light energy and fix CO2 across environmentally diverse and dynamic surface of our planet.

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