A novel thread-based microfluidic device for capillary electrophoresis with capacitively coupled contactless conductivity detection

Quero, Reverson Fernandes; Bressan, Lucas Paines; Silva, José Alberto Fracassi da; Jesus, Dosil Pereira de

doi:10.1016/j.snb.2019.01.168

Cited by 41 publications

(15 citation statements)

References 33 publications

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“…MCE devices can be fabricated over a wide range of platforms including glass , polymers , and other cheaper substrates like paper and threads . Different substrate materials have been selected for the development of NAME devices.…”

Section: Theoretical Aspectsmentioning

confidence: 99%

Nonaqueous electrophoresis on microchips: A review

2019

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The use of organic solvents as electrolytic medium in electrophoresis has become an important alternative for the analysis of compounds that exhibit low or no solubility in water. In recent years, nonaqueous electrophoresis has been extensively explored in conventional capillary systems for different applications. On the other hand, this separation strategy is still not as popular as free solution electrophoresis on chip-based platforms due to the effects of solvent in the background electrolyte on the sample injection, detection performance, and microfluidic platform compatibility. In this way, this review summarizes the main achievements on nonaqueous microchip electrophoresis (NAME). To the best of our knowledge, this is the first review dedicated to discuss exclusively nonaqueous electrophoresis on chip-based systems. For this purpose, some important theoretical aspects involved when separations are performed in organic medium, such as equilibrium, interactions and electrophoretic considerations, are included in the review. In addition, the main challenges, advantages and influences of nonaqueous media on the sample injection, detection as well as the choice of the substrate to fabricate chip-based electrophoresis devices are highlighted. Last, examples showing the feasibility of nonaqueous microchip electrophoresis for applications exploiting different methodologies, operational, and instrumental conditions are summarized and discussed. We hope this review can be useful to spread the huge potential of nonaqueous electrophoresis on microfluidic platforms.

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Section: Theoretical Aspectsmentioning

confidence: 99%

Nonaqueous electrophoresis on microchips: A review

2019

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“…Thus, in microfluidic devices, the measurement of

is in dilemmatic situation. This fact causes difficulty in lowering the working frequency of the C 4 D sensor and difficulty in improving the LOD of the conventional C 4 D sensor (the working frequency of conventional C 4 D sensor is usually in the 100 s of kHz or even in the MHz range) [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…Huck et al and Zhang et al have achieved great progress, which makes it possible for C 4 D sensors to approach a working frequency of less than 50 kHz effectively, and provides useful references for other researchers. However, in the field of microfluidic devices, the dielectric constants of materials (e.g., glass, PMMA and PDMS) are usually not high [ 16 , 17 , 18 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. It is difficult to introduce shielding into microfluidic devices and the optimization of electrodes and the channel size will more or less introduce some limitations in practical applications [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, in the field of microfluidic devices, the dielectric constants of materials (e.g., glass, PMMA and PDMS) are usually not high [ 16 , 17 , 18 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. It is difficult to introduce shielding into microfluidic devices and the optimization of electrodes and the channel size will more or less introduce some limitations in practical applications [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. Meanwhile, recently, few research works which use C 4 D sensors at a low working frequency and investigate conductivity of less than 0.2 mS/cm have been reported [ 16 , 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

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A Low Excitation Working Frequency Capacitively Coupled Contactless Conductivity Detection (C4D) Sensor for Microfluidic Devices

Huang

et al. 2021

Sensors

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In this work, a new capacitively coupled contactless conductivity detection (C4D) sensor for microfluidic devices is developed. By introducing an LC circuit, the working frequency of the new C4D sensor can be lowered by the adjustments of the inductor and the capacitance of the LC circuit. The limits of detection (LODs) of the new C4D sensor for conductivity/ion concentration measurement can be improved. Conductivity measurement experiments with KCl solutions were carried out in microfluidic devices (500 µm × 50 µm). The experimental results indicate that the developed C4D sensor can realize the conductivity measurement with low working frequency (less than 50 kHz). The LOD of the C4D sensor for conductivity measurement is estimated to be 2.2 µS/cm. Furthermore, to show the effectiveness of the new C4D sensor for the concentration measurement of other ions (solutions), SO42− and Li+ ion concentration measurement experiments were also carried out at a working frequency of 29.70 kHz. The experimental results show that at low concentrations, the input-output characteristics of the C4D sensor for SO42− and Li+ ion concentration measurement show good linearity with the LODs estimated to be 8.2 µM and 19.0 µM, respectively.

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“…Recent work by Quero et al. reports capacitively coupled contactless conductivity detection using copper electrodes on a thread platform for ion detection [22].…”

Section: Introductionmentioning

confidence: 99%

Electrophoresis on a polyester thread coupled with an end‐channel pencil electrode detector

2021

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We present the design and characterization of a low cost, thread‐based electrophoretic device with integrated electrochemical detection. The device has an end‐channel pencil graphite electrode placement system for performing electrochemical detection on the thread electrophoresis platform with direct sample pipetting onto the thread. We also established the use of methylene blue and neutral red as a pair of reference migration markers for separation techniques coupled with electrochemical detection, as they have different colors for visual analysis and are both electroactive. Importantly, neutral red was also found to migrate at a similar rate to the EOF, indicating that it can be used as a visual identifier of EOF. The utility of our system was demonstrated by electrophoretic separation and electrochemical detection of physiologically relevant concentrations of pyocyanin in a solution containing multiple electroactive compounds. Pyocyanin is a biomarker for the detection of pathogenic Pseudomonas aeruginosa and has a redox potential that is similar to that of methylene blue. The system was able to effectively resolve methylene blue, neutral red, and pyocyanin in less than 7 min of electrophoretic separation. The theoretical limit of detection for pyocyanin was determined to be 559 nM. The electrophoretic mobilities of methylene blue (0.0236 ± 0.0007 mm2/V·s), neutral red (0.0149 ± 0.0007 mm2/V·s), and pyocyanin (0.0107 ± 0.0003 mm2/V·s) were also determined.

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A novel thread-based microfluidic device for capillary electrophoresis with capacitively coupled contactless conductivity detection

Cited by 41 publications

References 33 publications

Nonaqueous electrophoresis on microchips: A review

Nonaqueous electrophoresis on microchips: A review

A Low Excitation Working Frequency Capacitively Coupled Contactless Conductivity Detection (C4D) Sensor for Microfluidic Devices

Electrophoresis on a polyester thread coupled with an end‐channel pencil electrode detector

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