A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis

Cha, Joon‐Yung; Kim, Woe‐Yeon; Kang, Sun Bin; Kim, Jeong Im; Baek, Dongwon; Jung, In‐Ha; Kim, Mi Ri; Li, Ning; Kim, Hyun‐Jin; Nakajima, Miki; Asami, Tadao; Sabir, Jamal S. M.; Park, Hyeong Cheol; Lee, Sang Yeol; Bohnert, Hans J.; Bressan, Ray A.; Pardo, José M.; Yun, Dae‐Jin

doi:10.1038/ncomms9041

Cited by 85 publications

(98 citation statements)

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“…48) YUC6 expression is reported in both roots and shoots. 49) YUC7 is expressed in roots and induced in response to drought stress. 37) YUC3 and 9 are expressed in the epidermis of the meristematic region of root tissue and are related to brassinosteroid signaling.…”

Section: Inhibition Of the Scf Tir1/afb -Signaling Pathway Increases mentioning

confidence: 99%

Auxin signaling through SCFTIR1/AFBs mediates feedback regulation of IAA biosynthesis

Takato

Kakei

Mitsui

et al. 2017

Bioscience, Biotechnology, and Biochemistry

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We previously reported that exogenous application of auxin to Arabidopsis seedlings resulted in downregulation of indole-3-acetic acid (IAA) biosynthesis genes in a feedback manner. In this study, we investigated the involvement of the SCF-mediated signaling pathway in feedback regulation of the indole-3-pyruvic acid-mediated auxin biosynthesis pathway in Arabidopsis. Application of PEO-IAA, an inhibitor of the IAA signal transduction pathway, to wild-type seedlings resulted in increased endogenous IAA levels in roots. Endogenous IAA levels in the auxin-signaling mutants axr2-1, axr3-3, and tir1-1afb1-1afb2-1afb3-1 also increased. Furthermore, YUCCA (YUC) gene expression was repressed in response to auxin treatment, and expression of YUC7 and YUC8 increased in response to PEO-IAA treatment. YUC genes were also induced in auxin-signaling mutants but repressed in TIR1-overexpression lines. These observations suggest that the endogenous IAA levels are regulated by auxin biosynthesis in a feedback manner, and the Aux/IAA and SCF-mediated auxin-signaling pathway regulates the expression of YUC genes.

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Section: Inhibition Of the Scf Tir1/afb -Signaling Pathway Increases mentioning

confidence: 99%

Auxin signaling through SCFTIR1/AFBs mediates feedback regulation of IAA biosynthesis

Takato

Kakei

Mitsui

et al. 2017

Bioscience, Biotechnology, and Biochemistry

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“…4C). The conversion of Indole Pyruvic Acid (IPA) into IAA is dependent on NADPH-dependent reduction [28] and it may be that reduced photosynthetic output limits auxin biosynthesis.…”

Section: Resultsmentioning

confidence: 99%

Disruption ofCYCLOPHILIN 38function reveals a photosynthesis-dependent systemic signal controlling lateral root emergence

Duan

Pérez-Ruiz

Cejudo

et al. 2020

Preprint

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15Photosynthesis in leaves generates the fixed-carbon resources and essential 16 metabolites that support sink tissues, such as roots [1]. One of these products, sucrose, 17 is known to promote primary root growth, but it is not clear what other molecules may be 18 involved and whether other stages of root system development are affected by 19 photosynthate levels [2]. Through a mutant screen to identify pathways regulating root 20 system architecture, we identified a mutation in the CYCLOPHILIN 38 (CYP38) gene, 21 which causes an accumulation of pre-emergent stage lateral roots, with a minor effect on 22 primary root growth. CYP38 was previously reported to maintain the stability of 23 Photosystem II (PSII) in chloroplasts [3]. CYP38 expression is enriched in the shoot and 24 grafting experiments show that the gene acts non-cell autonomously to promote lateral 25 root emergence. Growth of wild-type plants under low light conditions phenocopied the 26 cyp38 lateral root emergence phenotype as did the inhibition of PSII-dependent electron 27 transport or NADPH production. Importantly, the cyp38 root phenotype is not rescued 28 by exogenous sucrose, suggesting the involvement of another metabolite. Auxin (IAA) is 29 an essential hormone promoting root growth and its biosynthesis from tryptophan is 30 dependent on reductant generated during photosynthesis [4,5]. Both WT seedlings 31 grown under low light and cyp38 mutants have highly diminished levels of IAA in root 32 tissues. The cyp38 lateral root defect is rescued by IAA treatment, revealing that 33 photosynthesis promotes lateral root emergence partly through IAA 34 biosynthesis. Metabolomic profiling shows that the accumulation of several defense-35 related metabolites are also photosynthesis-dependent, suggesting that the regulation of 36 a number of energy-intensive pathways are down-regulated when light becomes limiting. 37Results and Discussion: 38CYP38 is necessary for lateral root emergence 39 Lateral roots form through oriented cell divisions within the pericycle tissue layer, hidden 40 3-layers deep inside the parent root [6]. The process of lateral root primordia 41 emergence through these outer tissues involves communication between the organ identify the causative genetic locus, CYPLOPHILLIN 38 (CYP38) (Figure. S1B). A T-49 DNA allele (cyp38-5) with an insertion in the sixth exon phenocopied the EMS allele 50 (cyp38-4) (Figure. 1A-C), which has an early stop codon in the second exon (Figure.51 1D). Expression of CYP38 using its native promoter fully complement cyp38-4 (Figure.52 1E), indicating that CYP38 is indeed the causative gene. 54To determine the developmental basis for the cyp38 phenotype, we classified lateral root 55 growth into 4 distinct stages: initiation (I), pre-emergence (Pre-E), emergence (E), and 56 maturation (M) (Figure. S1A). Compared to wild type (WT), where the majority of lateral 57 roots have typically progressed to the maturation stage in 8-dpg (days post germination) 58 seedlings, we found that the cyp38-5 mutants had a si...

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“…Presence absence variation among defence response (biotic stress) genes was previously observed among many plant species Li et al, 2014;Xu et al, 2012). PAV of genes involved in water homeostasis may be related to drought stress tolerance (Cha et al, 2015;Harb et al, 2010;Woo et al, 2007). Additionally, PAV among genes involved in signalling has also been observed Enrichment of signalling and defence response genes may be inter-related as complex signalling pathways are activated upon biotic stress detection (Hettenhausen et al, 2015;Meng and Zhang, 2013).…”

Section: An Example Of On Open Pangenome Ismentioning

confidence: 97%

Construction and analysis of the Brassica oleracea pangenome

Golicz¹

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This thesis describes the construction and analysis of a first Brassica oleracea pangenome. It also presents a pipeline that was developed and implemented in order to build and analyse the pangenome.B. oleracea is a species of high economic importance encompassing crops like broccoli, Brussels sprout, cabbage, cauliflower, kale and kohlrabi. Additionally, it is of high interest to the scientific community because the crops display remarkable morphological and physiological variation and the genetic underpinning of this variation is not fully understood.Recent years have seen an explosion of genome sequencing projects. It has also become clear that there is a significant amount of presence/absence and copy number variation between individuals of the same species, and a reference sequence from a single individual is unable to capture all of the genomic information necessary to fully characterize a species. The term pangenome was coined to describe all the genomic (genic) sequence information found within species.We have hypothesized that some of the phenotypic variation found in the B. oleracea crops might be due to presence/absence variants and therefore we have set out to construct and analyse the B. oleracea pangenome.The pipeline designed was composed of three main steps 1. Pangenome assembly using an iterative mapping and assembly approach and pangenome annotation. Chalhoub, B., Denoeud, F., Liu, S., Parkin, I.A.P., Tang, H., Wang, X., Chiquet, J., Belcram, H., Tong, C., Samans, B., Corréa, M., Da Silva, C., Just, J., Falentin, C., Koh, C.S., Le Clainche, I., Bernard, M., Bento, P., Noel, B., Labadie, K., Alberti, A., Charles, M., Arnaud, D., Guo, H., Daviaud, C., Alamery, S., Jabbari, K., Zhao, M., Edger, P.P., Chelaifa, H., Tack, D., Lassalle, G., Mestiri, I., Schnel, N., Le Paslier, M.-C., Fan, G., Renault, V., Bayer, P.E., Golicz, A.A., Manoli, S., Lee, T.-H., Thi, V.H.D., Chalabi, S., Hu, Q., Fan, C., Tollenaere, R., Lu, Y., Battail, C., Shen, J., Sidebottom, C.H.D., Wang, X., Canaguier, A., Chauveau, A., Bérard, A., Deniot, G., Guan, M., Liu, Z., Sun, F., Lim, Y.P., Lyons, E., Town, C.D., Bancroft, I., Wang, X., Meng, J., Ma, J., Pires, J.C., King, G.J., Brunel, D., Delourme, R., Renard, M.,

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A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis

Cited by 85 publications

References 70 publications

Auxin signaling through SCFTIR1/AFBs mediates feedback regulation of IAA biosynthesis

Auxin signaling through SCFTIR1/AFBs mediates feedback regulation of IAA biosynthesis

Disruption ofCYCLOPHILIN 38function reveals a photosynthesis-dependent systemic signal controlling lateral root emergence

Construction and analysis of the Brassica oleracea pangenome

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