A novel thick filament protein in smooth muscles of bivalve molluscs

Shelud’ko, Nikolay S.; Tuturova, K.F.; Permyakova, T. V.; Plotnikov, Sergey V.; Orlova, Albina

doi:10.1016/s0305-0491(99)00009-7

Cited by 32 publications

(18 citation statements)

References 28 publications

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“…1971; Cohen 1 982). Another protein, essential for the ‘catch’ state, is myorod, a myosin rod‐like protein (Shelud’ko et al . 1999); it is expressed by alternative splicing from the myosin heavy chain (MHC) gene in ‘catch’‐muscle of bivalves (Yamada et al .…”

Section: Introductionmentioning

confidence: 99%

“…Paramyosin is wrapped in a monolayer of myosin (Szent-Gyorgyi et al 1971;Cohen 1982). Another protein, essential for the 'catch' state, is myorod, a myosin rodlike protein (Shelud'ko et al 1999); it is expressed by alternative splicing from the myosin heavy chain (MHC) gene in 'catch'-muscle of bivalves (Yamada et al 2000). The giant muscle protein twitchin is located on the surface of the thick filaments in the molluscan 'catch' muscles (Vibert et al 1993) and regulates the 'catch' phenomenon in a phosphorylation manner Siegman et al 1998).…”

Section: Introductionmentioning

confidence: 99%

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Development of the larval muscle system in the mussel Mytilus trossulus (Mollusca, Bivalvia)

Dyachuk

Odintsova

2009

Dev Growth Differ

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In the present study we examined muscle development throughout the complete larval cycle of the bivalve mollusc, Mytilus trossulus. An immunofluorescence technique and laser scanning confocal microscopy were used in order to study the organization of the muscle proteins (myosin, paramyosin, twitchin, and actin) and some neurotransmitters. The appearance of the muscle bundles lagged behind their nervous supply: the neuronal elements developed slightly earlier (by 2 h) than the muscle cells. The pioneer muscle cells forming a prototroch muscle ring were observed in a completed trochophore. We documented a well-organized muscle system that consisted of the muscle ring transforming into three pairs of velar striated retractors in the early veliger. The striations were positive for all muscle proteins tested. Distribution of FMRFamide and serotonin (5-HT) immunocytochemical staining relative to the muscle ring differed significantly: 5-HT-immunioreactive cells were situated in the center of the striated muscle ring, while Phe-Met-Arg-Phe-NH2 neuropeptide FMRFamid immunoreactive fibers were located in a distal part of this ring. Our data showed clearly that the muscle proteins and the neurotransmitters were co-expressed in a coordinated fashion in a continuum during the early stages of the mussel development. Our study provides the first strong evidence that mussel larval metamorphosis is accompanied by a massive reorganization of striated muscles, followed by the development of smooth muscles capable of catch-contraction.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of the larval muscle system in the mussel Mytilus trossulus (Mollusca, Bivalvia)

Dyachuk

Odintsova

2009

Dev Growth Differ

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“…The larval muscle system contains both striated and smooth muscles (Cragg and Crisp 1991;Dyachuk and Odintsova 2009), whereas the adult musculature is simple and consists entirely of smooth muscle cells with an unusual structure of their thick filaments (Szent-Gyorgyi et al 1971). Molluscan smooth muscles have a unique protein composition: their thick filaments are formed by paramyosin, myosin, twitchin, and myorod (Levine et al 1976;Vibert et al 1993;Shelud'ko et al 1999;Odintsova et al 2006). The last three proteins are located around the paramyosin core (Winkleman 1976;Royuela et al 1997;Scott et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Muscle and neuronal differentiation in primary cell culture of larval Mytilus trossulus (Mollusca: Bivalvia)

2010

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Molluscan in vitro technology allows the study of the differentiation of isolated cells undergoing experimental manipulations. We have used the immunofluorescence technique and laser scanning microscopy to investigate the organization of muscle proteins (actin, myosin, paramyosin, and twitchin) and the localization of neurotransmitters (serotonin and FMRFamide) in cultured mussel larval cells. Differentiation into muscle and neuron-like cells occurs during the cultivation of mussel cells from premyogenic and prenervous larval stages. Muscle proteins are colocalized in contractile cells through all stages of cultivation. The cultivation of mussel cells on various substrates and the application of integrin receptor blockers suggest that an integrin-dependent mechanism is involved in cell adhesion and differentiation. Dissociated mussel cells aggregate and become self-organized in culture. After 20 days of cultivation, they form colonies in which serotonin- and FMRFamide-immunoreactive cells are located centrally, whereas muscle cells form a contractile network at the periphery. The pattern of thick and thin filaments in cultivated mussel cells changes according to the scenario of muscle arrangement in vivo: initially, a striated pattern of muscle filaments forms but is then replaced by a smooth muscle pattern with a diffuse distribution of muscle proteins, typical of muscles of adult molluscs. Myogenesis in molluscs thus seems to be a highly dynamic and potentially variable process. Such a "flexible" developmental program can be regarded as a prerequisite for the evolution of the wide variety of striated and smooth muscles in larval and adult molluscs.

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“…The proteins were separated by 8 % SDS polyacrylamide slab gel electrophoresis, as described by Laemmli (1970) but with modifications described by Shelud'ko et al (1999).…”

Section: Sds-page and Wb Analysismentioning

confidence: 99%

Extracellular matrix is required for muscle differentiation in primary cell cultures of larval Mytilus trossulus (Mollusca: Bivalvia)

Dyachuk

2013

Cytotechnology

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Components of the extracellular matrix may modulate the growth factor effects that play important roles in the proliferation and differentiation of precursor cells. We developed an in vitro cultivation protocol for cells of the larval marine bivalve Mytilus trossulus to study the role that extracellular matrix components may play in myodifferentiation and replication-mediated DNA synthesis using immunofluorescence and confocal laser scanning microscopy. Here, we demonstrate that the extracellular matrix regulates the expression of muscle proteins, leading to their assembly and the terminal muscle differentiation of larval cells during cultivation. We further show that the myogenesis process progresses in cells cultivated on fibronectin, carbon or poly-L-lysine but is inhibited in cells grown on a collagen carpet. Consistent with a decrease in muscle protein expression in cells cultivated on collagen, we demonstrate an increase in the number of BrdU-positive cells in comparison with cells cultured on other substrates during the entire cultivation period. Moreover, we demonstrate that the matrix-dependent myogenic differentiation of larval mussel cells is reversible. Round-shaped cells cultivated on collagen were able to differentiate into muscle cells after reseeding on fibronectin, carbon or poly-L-lysine. In addition, cells cultured on collagen and then transplanted to fibronectin exhibited distinct cross-striation and contractile activity. Taken together, our data suggest that the extracellular matrix participates in the regulation of the proliferation and myodifferentiation of mussel trochophore progenitor cells and validate novel approaches for successfully culturing cells from bivalves over extended periods.

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A novel thick filament protein in smooth muscles of bivalve molluscs

Cited by 32 publications

References 28 publications

Development of the larval muscle system in the mussel Mytilus trossulus (Mollusca, Bivalvia)

Development of the larval muscle system in the mussel Mytilus trossulus (Mollusca, Bivalvia)

Muscle and neuronal differentiation in primary cell culture of larval Mytilus trossulus (Mollusca: Bivalvia)

Extracellular matrix is required for muscle differentiation in primary cell cultures of larval Mytilus trossulus (Mollusca: Bivalvia)

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