A Novel Technology for Hematopoietic Stem Cell Expansion Using Combination of Nanofiber and Growth Factors

Lu, Jingwei; Aggarwal, Rashmi; Pompili, Vincent J.; Das, Hiranmoy

doi:10.2174/187221010791208777

Cited by 27 publications

(16 citation statements)

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“…1,2 Perhaps not surprisingly, human cells appear to proliferate robustly on nanostructures, and this phenomenon can afford the opportunity for detailed evaluation of cellular growth mechanisms. [3][4][5] The central objective of fabricating nanostructures for biological applications is the creation of compatible and biodegradable three-dimensional scaffolds upon which human cells can either differentiate, divide, or both. Polycaprolactone (PCL), a hydrophobic polymer, is a well-known biocompatible polymer on which to grow human cells and PCL nanofiber scaffolds can be produced by electrospinning.…”

Section: Introductionmentioning

confidence: 99%

“…5,7,12 It is believed that, as nanofibers resemble an extracellular matrix, at least structurally, they can provide multilayered physical support for stem cell adhesion. 26,27 While this approach has provided dynamic and exciting results for hematopoietic stem cells, 5 neuronal stem cells, 28 and mesenchymal stem calls, 7,29 relatively little is known about how such nanofiber scaffolds enable the recovery and re-ignition of the proliferation of genetically modified human cells. These cells are particularly relevant to nanomedicine because they are the product of ex vivo gene therapy approaches.…”

Section: Introductionmentioning

confidence: 99%

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Electrospun fiber membranes enable proliferation of genetically modified cells

Borjigin¹,

Eskridge²,

Niamat³

et al. 2013

IJN

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Polycaprolactone (PCL) and its blended composites (chitosan, gelatin, and lecithin) are well-established biomaterials that can enrich cell growth and enable tissue engineering. However, their application in the recovery and proliferation of genetically modified cells has not been studied. In the study reported here, we fabricated PCL-biomaterial blended fiber membranes, characterized them using physicochemical techniques, and used them as templates for the growth of genetically modified HCT116-19 colon cancer cells. Our data show that the blended polymers are highly miscible and form homogenous electrospun fiber membranes of uniform texture. The aligned PCL nanofibers support robust cell growth, yielding a 2.5-fold higher proliferation rate than cells plated on standard plastic plate surfaces. PCL-lecithin fiber membranes yielded a 2.7-fold higher rate of proliferation, while PCL-chitosan supported a more modest growth rate (1.5-fold higher). Surprisingly, PCL-gelatin did not enhance cell proliferation when compared to the rate of cell growth on plastic surfaces.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrospun fiber membranes enable proliferation of genetically modified cells

Borjigin¹,

Eskridge²,

Niamat³

et al. 2013

IJN

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“…+ cord blood cells [84,85]. Other expansion 3D-approaches made use of nonwoven polyethylene terephthalate (PET).…”

Section: Electrospun Nanofibresmentioning

confidence: 99%

Artificial Hematopoietic Stem Cell Niches-Dimensionality Matters

Gottwald¹

2017

ATROA

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Hematopoietic stem cell niches are perhaps the best described niche system in mammals. The niches themselves, as well as their cellular and structural constituents and factors that play a role in maintaining the niche structure and function, are being refined on a more or less daily basis. Despite this, it seems as if the more we know the harder it gets to mimic the in vivo-situation using in vitro-systems. This is due to the fact that hematopoiesis is a multi step maturation process leading to HSC heterogeneity. Subpopulations of HSCs and niche supporting cells can be defined depending on characteristics such as their potency of leading to successful reconstitution of sub lethally irradiated mice in serial transplantation experiments or, with less scientific impact, clonogenic assays. Since the bone marrow obviously provides all necessary information to maintain the stem cell pool constant and to adapt the number of blood cells according to physiologic needs, it has been the goal to engineer artificial niches that display at least one or several of the major characteristics of the in vivosituation to make use of these systems for not only fundamental research purposes but, moreover, also for clinical applications. This review will give an overview of approaches to engineering artificial hematopoietic niches with a focus on the complexity/dimensionality of the systems used.

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“…Extensive studies have been published regarding the impact of nanotechnology in a broad spectrum of stem cell types, including pluripotent [47], neural [48,49], and hematopoietic [50] cells-driven tissue engineering.…”

Section: Accepted Manuscriptmentioning

confidence: 99%