A novel technique of using mechanical grippers to immobilize embryo during biopsy

Abstract

Patients underwent intracytoplasmic sperm injection and had at least one GV oocyte after denudation were included. After denudation, GV oocytes were cultured in G-IVFÒ media and placed into a time-lapse incubator. Images were taken every 10 mins for 144 hours. The GVBD and PBE time were counted. Patient's age, protocol, base and hCG day luteinizing hormone (LH), base follicle stimulation hormone (FSH), base and hCG day estradiol (E2), immaturation rate, and big follicle acquisition rate (BFA, BFA¼[N of retrieval oocyte]/[N of folli-clesR12mm]) were recorded for univariable clustered Cox regression [1,2]. Variables (p <0.3) were chosen for multivariable analysis. Hazard ratio (HR) with 95% confidence interval (95%CI) were reported.RESULTS: There were 36 patients (79 GV oocytes) recruited. 12 GV oocytes did not mature. The overall time-course was 23.2h (95%CI 21.3-24.4h). The baseline and analysis results are shown in Table I. The BFA<1 means GV oocytes were from big follicles. GV oocytes in group BFA<1 showed shortened time-course in both univariable (HR 2.43, 95%CI 1.49-4.35, p< .001) and multivariable analysis (HR 2.38, 95%CI 1.32-4.35, p¼0.004). The adjusted chance of maturation in group base E2 concentration >50 was revealed to be two times greater (HR 2.00 95%CI 1.06-3.81, p¼0.034).CONCLUSIONS: We first demonstrate a precise time-course of GVBD to PBE in stimulated cycles, which can contribute significantly to catching the fertilization window in r-IVM as well as traditional IVM. We found that GV oocytes from big follicles or patient with higher base E2 have higher chance of maturation. These findings give clues for oocyte and follicle development, but further studies are needed to confirm.References: [1] Moore, D. F. (2016). Applied survival analysis using R, Springer.[2] Glidden, D. V. and E. Vittinghoff "Modelling clustered survival data from multicentre clinical trials." Statistics in medicine 23(3): 369-388. (2004).''

A novel device to micromanipulate oocytes during intracytoplasmic sperm injection

“…The fragility of the glass pipette is a challenging thing for any novice embryologist in doing ICSI using the HP; also, the harm caused to the oocyte due to the suction using HP is not negligible and the usage of a gripper can potentially solve this problem. [15] A total of 40 ICSIs were carried out on matured human oocytes and we found zero degeneration rate and the distribution of post ICSI oocytes and the embryo grading is as shown in [Figures 14 and 15] which show the graph which emphasizes the morphology-based health of the oocytes before doing ICSI. [Figure 16] denotes all the Nomenclature with abbreviations used in this article along with their fullforms.…”