A Novel Sweetpotato WRKY Transcription Factor, IbWRKY2, Positively Regulates Drought and Salt Tolerance in Transgenic Arabidopsis

Zhu, Hong; Zhou, Yuanyuan; Zhai, Hong; He, Shaozhen; Zhao, Ning; Liu, Qingchang

doi:10.3390/biom10040506

Cited by 66 publications

(36 citation statements)

References 67 publications

(82 reference statements)

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“…BhWRKY1 protein in Boea hygrometrica binds to the promoter of BhGolS1 and is associated with both low-temperature resistance and drought tolerance [60]. IbWRKY2 can interact with IbVQ4, and drought and salt treatment can induce the expression of IbVQ4, thus improving the tolerance of plants to drought and salt stress [69]. MdWRKY30 overexpression enhances tolerance to salt and osmotic stress in transgenic apple callus through transcriptional regulation of stress-related genes [70].…”

Section: Wrky Tfs and Other Abiotic Stressesmentioning

confidence: 99%

Function and Mechanism of WRKY Transcription Factors in Abiotic Stress Responses of Plants

Pang

et al. 2020

Plants

175

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The WRKY gene family is a plant-specific transcription factor (TF) group, playing important roles in many different response pathways of diverse abiotic stresses (drought, saline, alkali, temperature, and ultraviolet radiation, and so forth). In recent years, many studies have explored the role and mechanism of WRKY family members from model plants to agricultural crops and other species. Abiotic stress adversely affects the growth and development of plants. Thus, a review of WRKY with stress responses is important to increase our understanding of abiotic stress responses in plants. Here, we summarize the structural characteristics and regulatory mechanism of WRKY transcription factors and their responses to abiotic stress. We also discuss current issues and future perspectives of WRKY transcription factor research.

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Section: Wrky Tfs and Other Abiotic Stressesmentioning

confidence: 99%

Function and Mechanism of WRKY Transcription Factors in Abiotic Stress Responses of Plants

Pang

et al. 2020

Plants

175

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“…They often function as central regulators and molecular switches that activate or repress transcription of multiple target genes [ 13 , 14 ]. The WRKY gene family, one of the largest families of transcription factors, has received increasing attention for its members’ roles in plant growth, regulation of defense responses, and stress responses [ 15 – 17 ]. WRKY proteins, which apparently exist exclusively in plants, share a WRKY domain (WD) that is comprised of about 60 amino acid residues [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression analysis of the WRKY transcription factor family in flax (Linum usitatissimum L.)

et al. 2021

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Background Members of the WRKY protein family, one of the largest transcription factor families in plants, are involved in plant growth and development, signal transduction, senescence, and stress resistance. However, little information is available about WRKY transcription factors in flax (Linum usitatissimum L.). Results In this study, comprehensive genome-wide characterization of the flax WRKY gene family was conducted that led to prediction of 102 LuWRKY genes. Based on bioinformatics-based predictions of structural and phylogenetic features of encoded LuWRKY proteins, 95 LuWRKYs were classified into three main groups (Group I, II, and III); Group II LuWRKYs were further assigned to five subgroups (IIa-e), while seven unique LuWRKYs (LuWRKYs 96–102) could not be assigned to any group. Most LuWRKY proteins within a given subgroup shared similar motif compositions, while a high degree of motif composition variability was apparent between subgroups. Using RNA-seq data, expression patterns of the 102 predicted LuWRKY genes were also investigated. Expression profiling data demonstrated that most genes associated with cellulose, hemicellulose, or lignin content were predominantly expressed in stems, roots, and less in leaves. However, most genes associated with stress responses were predominantly expressed in leaves and exhibited distinctly higher expression levels in developmental stages 1 and 8 than during other stages. Conclusions Ultimately, the present study provides a comprehensive analysis of predicted flax WRKY family genes to guide future investigations to reveal functions of LuWRKY proteins during plant growth, development, and stress responses.

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“…The WRKY TF family is one of the largest families of plant-specific proteins and plays important roles in the response to different abiotic stresses and the ABA signaling pathway. For example, overexpression of wheat TaWRKY2 and TaWRKY19 , soybean GmWRKY12 , GmWRKY16 and GmWRKY54 , or sweet potato IbWRKY2 in transgenic plants results in enhanced salt and drought tolerance [ 26 , 27 , 28 , 29 , 30 ]. OsWRKY45 alleles play different roles in ABA signaling, OsWRKY45-1 is insensitive to salt stress in rice, and OsWRKY45-2 negatively regulates the response to salt stress [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

“…OsWRKY45 alleles play different roles in ABA signaling, OsWRKY45-1 is insensitive to salt stress in rice, and OsWRKY45-2 negatively regulates the response to salt stress [ 31 ]. Transgenic plants overexpressing cotton GhWRKY6 -like gene or sweet potato IbWRKY2 display enhanced salt tolerance by regulating the ABA signaling pathway and ROS scavenging [ 30 , 32 ]. Recently, the expression of FtWRKY46 from buckwheat, DgWRKY4 and DgWRKY5 from chrysanthemum, and GarWRKY5 from cotton in transgenic plants was shown to confer enhanced tolerance to salt stress by modulating ROS clearance, antioxidant enzyme activity, and stress-related gene expression [ 6 , 33 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by Directly Regulating ThWRKY4

Sun

Wang

et al. 2021

IJMS

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Heat shock transcription factors (HSFs) play critical roles in several types of environmental stresses. However, the detailed regulatory mechanisms in response to salt stress are still largely unknown. In this study, we examined the salt-induced transcriptional responses of ThHSFA1-ThWRKY4 in Tamarix hispida and their functions and regulatory mechanisms in salt tolerance. ThHSFA1 protein acts as an upstream regulator that can directly activate ThWRKY4 expression by binding to the heat shock element (HSE) of the ThWRKY4 promoter using yeast one-hybrid (Y1H), chromatin immunoprecipitation (ChIP), and dual-luciferase reporter assays. ThHSFA1 and ThWRKY4 expression was significantly induced by salt stress and abscisic acid (ABA) treatment in the roots and leaves of T. hispida. ThHSFA1 is a nuclear-localized protein with transactivation activity at the C-terminus. Compared to nontransgenic plants, transgenic plants overexpressing ThHSFA1 displayed enhanced salt tolerance and exhibited reduced reactive oxygen species (ROS) levels and increased antioxidant enzyme activity levels under salt stress. Therefore, we further concluded that ThHSFA1 mediated the regulation of ThWRKY4 in response to salt stress in T. hispida.

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A Novel Sweetpotato WRKY Transcription Factor, IbWRKY2, Positively Regulates Drought and Salt Tolerance in Transgenic Arabidopsis

Cited by 66 publications

References 67 publications

Function and Mechanism of WRKY Transcription Factors in Abiotic Stress Responses of Plants

Function and Mechanism of WRKY Transcription Factors in Abiotic Stress Responses of Plants

Genome-wide identification and expression analysis of the WRKY transcription factor family in flax (Linum usitatissimum L.)

ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by Directly Regulating ThWRKY4

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