A novel surface plasmon resonance biosensor based on graphene oxide decorated with gold nanorod–antibody conjugates for determination of transferrin

Zhang, Jia; Sun, Ying; Xu, Bo; Zhang, Hua; Gao, Yan; Zhang, Hanqi; Song, Daqian

doi:10.1016/j.bios.2013.02.008

Cited by 106 publications

(39 citation statements)

References 37 publications

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“…The use of nanomaterials in signal enhancement for the detection of an analyte in SPR has been intensely studied. Most commonly applied nanomaterials include latex nanoparticles [38], metallic nanoparticles e.g., gold and silver nanoparticles [39,40], magnetic nanoparticles [41,42], carbon-based nanostructures e.g., graphene [40,43] and liposome nanoparticles [41,44]. However, the use of the above nanoparticles in the enhancement of SPR-based assays of biological materials are predominantly for protein and small molecules.…”

Section: Sandwich Assay With Signal Amplification Using Aunpsmentioning

confidence: 99%

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

2017

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Abstract:Campylobacteriosis is an internationally important foodborne disease caused by Campylobacter jejuni. The bacterium is prevalent in chicken meat and it is estimated that as much as 90% of chicken meat on the market may be contaminated with the bacterium. The current gold standard for the detection of C. jejuni is the culturing method, which takes at least 48 h to confirm the presence of the bacterium. Hence, the aim of this work was to investigate the development of a Surface Plasmon Resonance (SPR) sensor platform for C. jejuni detection. Bacterial strains were cultivated in-house and used in the development of the sensor. SPR sensor chips were first functionalized with polyclonal antibodies raised against C. jejuni using covalent attachment. The gold chips were then applied for the direct detection of C. jejuni. The assay conditions were then optimized and the sensor used for C. jejuni detection, achieving a detection limit of 8 × 10 6 CFU·mL −1 . The sensitivity of the assay was further enhanced to 4 × 10 4 CFU·mL −1 through the deployment of a sandwich assay format using the same polyclonal antibody. The LOD obtained in the sandwich assay was higher than that achieved using commercial enzyme-linked immunosorbent assay (ELISA) (10 6 -10 7 CFU·mL −1 ). This indicate that the SPR-based sandwich sensor method has an excellent potential to replace ELISA tests for C. jejuni detection. Specificity studies performed with Gram-positive and Gram-negative bacteria, demonstrated the high specific of the sensor for C. jejuni.

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Section: Sandwich Assay With Signal Amplification Using Aunpsmentioning

confidence: 99%

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

2017

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“…GO has been conjugated with gold-Ab and modified the traditional surface plasmon resonance. GO provides a larger surface area and abundant oxygen molecules as a functional group benefiting the method of immobilization and detection sensitivity [44]. Go-based biosensors have been also used to detect ssDNA, living cells and metal ions [45].…”

Section: Biofunctionalization With Proteinsmentioning

confidence: 99%

“…For instance, doxorubicin (DOX)-GO compound indicated the appearance of DOX from GO which is pH sensitive because of DOX's higher solvency at low pH [65]. Considering this point of interest, pH-sensitive delivery was effectively exhibited in case of DOX and camptothecin by utilizing folic acid consisting of nano-GO (NGO), called FA-NGO to treat tumours [44]. Ibuprofen and 5-fluorouracil drugs, having antiinflammatory property with distinctive hydrophilicity, were likewise delivered by utilizing the CS-GO complex with a pH-dependent release [66].…”

Section: Small Molecule Drug Deliverymentioning

confidence: 99%

Graphene and graphene oxide as nanomaterials for medicine and biology application

et al. 2018

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Graphene-and graphene oxide-based nanomaterials have gained broad interests in research because of their unique physiochemical properties. The 2D allotropic structure allows it to be used in various biological fields. The biomedical applications of graphene and its composite include its use in gene and small molecular drug delivery. It is further used for biofunctionalization of protein, in anticancer therapy, as an antimicrobial agent for bone and teeth implantation. The biocompatibility of the newly synthesized nanomaterials allows its substantial use in medicine and biology. The current review summarizes the chemical structure and biological application of graphene in various fields.

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“…One of the promising developments is the usage of graphene surfaces which enable large specific sensor surface, long-term stability and immobilization of varieties of biomolecules through covalent, noncovalent or electrostatic interactions. 50 …”

Section: Methodsmentioning

confidence: 99%

How to Study Protein-protein Interactions

Podobnik¹,

Kraševec²,

Zavec³

et al. 2016

ACSi

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In memory of prof. dr. Janko Jamnik. AbstractPhysical and functional interactions between molecules in living systems are central to all biological processes. Identification of protein complexes therefore is becoming increasingly important to gain a molecular understanding of cells and organisms. Several powerful methodologies and techniques have been developed to study molecular interactions and thus help elucidate their nature and role in biology as well as potential ways how to interfere with them. All different techniques used in these studies have their strengths and weaknesses and since they are mostly employed in in vitro conditions, a single approach can hardly accurately reproduce interactions that happen under physiological conditions. However, complementary usage of as many as possible available techniques can lead to relatively realistic picture of the biological process. Here we describe several proteomic, biophysical and structural tools that help us understand the nature and mechanism of these interactions.

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A novel surface plasmon resonance biosensor based on graphene oxide decorated with gold nanorod–antibody conjugates for determination of transferrin

Cited by 106 publications

References 37 publications

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

Graphene and graphene oxide as nanomaterials for medicine and biology application

How to Study Protein-protein Interactions

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