A Novel Surface Modification and Immobilization Method of Anti-CD25 Antibody on Nonwoven Fabric Filter Removing Regulatory T Cells Selectively

Abstract

Anti-CD25 antibodies were immobilized on polypropylene (PP) nonwoven fabrics to specifically remove mouse regulatory T cells (Tregs) from mouse spleen cells. PP fibers were coated with peptide nanosheets, which were prepared by self-assembling of a mixture of X-poly­(sarcosine)-b-(l-Leu-Aib)6 (X: glycolic acid or a phenylboronic acid) and Y-poly­(sarcosine)-b-(d-Leu-Aib)6 (Y: glycolic acid or diazirine derivative). Anti-CD25 antibodies were immobilized by covalent linking between the sugar moiety of the antibody and the phenylboronic acid group on the peptide nanosheet. The removal rate of mouse Tregs from the mouse spleen cells was more than 95% only by passing the filters, while the nonspecific removal rates of other cells were less than 15%. The coating of peptide nanosheets on PP fibers was very effective to provide a suitable environment for the immobilized antibody to interact with the counterpart cells while the coating suppressed nonspecific adsorption of other cells.

A critical review of hemoperfusion adsorbents: materials, functionalization and matrix structure selection

“…57,58 membrane have higher LPS adsorption capacity than AN69 membrane, which is related to the high amount of cationic functional groups of PEI. 78,79 In addition, more advanced peptide nanosheets coating on polypropylene (PP) nonwoven fabrics was proposed by Uji et al 80 These peptide nanosheets have two faces, one for phenylboronic acid to connect to site-oriented antibodies to remove regulatory T cells specifically, and the other for photoactivable radical generator to react with PP fiber to improve the compatibility of PP fiber. This method established a strategy of highly biocompatible surface coating on pristine hemoperfusion materials, which pioneered the research of peptide and protein coating materials.…”

Well‐Defined Anisotropic Self‐Assembly from Peptoids and Their Biomedical Applications

“…Thus, their secondary structures and physicochemical properties are strongly determined by their sidechains, which allows modulation through side chain design. For example, peptoids comprising C1 or C2 alkyl side chains, i. e., poly( N‐ methylglycine) or poly( N ‐ethylglycine), are hydrophilic, whereas peptoids containing four or more carbons are hydrophobic, and poly( N ‐C3 glycines) exhibit a thermoresponsive behavior [1–6] . Secondary structures are also affected by side chains, with peptoids adopting a random coil structure in the presence of small side chains and assuming a helical conformation in the presence of bulky and chiral side chains [7,8] .…”

“…Moreover, several studies have shown that peptoids have low cytotoxicity [20–25] . Taking the advantages of these advantages, peptoids are applied to non‐fouling coatings, [6,26,27] protein‐purification components, [28–31] antibacterial agents, [32–39] and therapeutic agents [36,40–45] …”

Tumor growth suppression by implantation of an anti-CD25 antibody-immobilized material near the tumor via regulatory T cell capture

“…Several treatments that inhibit immunosuppressive signal transduction by immune checkpoint inhibitors (e.g., anti-CTLA-4 and anti-programmed death-1 antibodies) and depletion of Tregs by administration of anti-C-C motif chemokine receptor 4 antibodies have been proposed as Treg-related cancer immunotherapies [8,9]. The development of selective Treg removal methods is also proposed [10,11]. Although the efficacies of these treatments have been demonstrated, treatment with immune checkpoint inhibitors can induce serious side effects owing to activation of T cells [12].…”