A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice

Nehmi, Victor Abou; Murata, Gilson Masahiro; Moraes, Ruan Carlos Macêdo de; Lima, Gabriely Cristina Alves; Miranda, Danielle Araújo de; Radloff, Katrin; Costa, Raquel; Jesus, Joyce de Cassia Rosa de; Freitas, Jéssica Alves de; Viana, Nayara; Pimenta, Ruan; Leite, Kátia Ramos Moreira; Otoch, José Pinhata; Pessoa, Ana Flávia Marçal

doi:10.1016/j.joim.2021.05.002

Cited by 12 publications

(16 citation statements)

References 59 publications

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“…Despite the limitation posed by a modest sample size, our group's prior investigations have demonstrated compelling outcomes of this nutraceutical in preclinical models of obesity and type 2 diabetes, utilizing an identical sample size. [2][3][4] These earlier studies yielded statistically significant results, thus providing a strong foundation for the current study's rationale and potential implications. However, it is crucial to acknowledge the necessity for larger sample sizes in future research to further validate and consolidate these findings, ensuring greater generalizability and robustness of the conclusions drawn.…”

Section: Discussionmentioning

confidence: 66%

“…This treatment duration was chosen to assess the long-term effects of supplementation, as previously reported. 2 At the end of the experimental period, the animals were euthanized with an overdose of ketamine and xylazine, and fragments of the small intestine were collected for further analysis.…”

Section: Methodsmentioning

confidence: 99%

“…1 Notably, numerous studies have demonstrated the potential of nutraceuticals as effective therapeutic approaches for the prevention and management of various inflammatory and metabolic disorders, including but not limited to obesity, steatosis, and type-2 diabetes mellitus. [2][3][4] Silymarin (Silybum marianum) exerts hepatoprotective effects through various mechanisms, including antioxidant activity and stabilization of the hepatocellular membrane. 5 Furthermore, silymarin has demonstrated the ability to decrease plasma cholesterol and low-density lipoprotein levels in hyperlipidemic animals.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Modulation of Small-Intestine Morphology in Mice by a Novel Supplement Containing Silybum marianum, Yeast β-Glucan, Prebiotics, and Minerals

Nucci,

Nehmi-Filho,

Bastos

et al. 2023

Journal of Health and Allied Sciences NU

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Silymarin, derived from Silybum marianum, has recently demonstrated its potential to improve health in conditions such as obesity and metabolic disturbances. Understanding the impact of nutraceuticals on intestinal morphology is crucial for developing supplements that promote a higher quality of life. Therefore, this study aimed to investigate the effects of nutraceutical supplementation with silymarin on the morphology of the small intestine. Sixty-day-old adult male C57BL/6 mice were divided into two groups: one receiving a standard chow (control) and the other receiving a novel silymarin supplement (experimental). Following the experimental period, the animals were euthanized, and fragments of the small intestine were collected for histochemical analysis using Masson's trichrome and periodic acid-Schiff with Alcian blue staining techniques. Our results revealed an increase in the number of villi per analyzed field in the experimental group, accompanied by a decrease in basic mucin, crypt depth, mucosal thickness, and villus spacing. In conclusion, this novel nutraceutical supplementation may play a crucial role in modulating small intestine morphology and enhancing absorption capacity.

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Section: Discussionmentioning

confidence: 66%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Modulation of Small-Intestine Morphology in Mice by a Novel Supplement Containing Silybum marianum, Yeast β-Glucan, Prebiotics, and Minerals

Nucci,

Nehmi-Filho,

Bastos

et al. 2023

Journal of Health and Allied Sciences NU

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“…Accumulating evidence supported that nuclear transcription factors such as peroxisome proliferator-activated receptors γ (PPAR γ ) [ 15 – 17 ] and lipid droplet-associated protein adipose differentiation-related protein (ADRP) [ 18 – 20 ] play important roles in the development of fatty liver. It is reported that ADRP gene transcription is regulated by PPARs in human hepatocytes, and both of them are upregulated in high-fat diet (HFD)-induced fatty liver [ 21 , 22 ]. Our previous study had proved that ICAE downregulated the protein level of PPAR γ and inhibited adipocyte differentiation in vitro [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Preventive Effects of Ilex Cornuta Aqueous Extract on High-Fat Diet-Induced Fatty Liver of Mice

Liu

Jia

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. To investigate the preventive effects of Ilex cornuta aqueous extract (ICAE) on high-fat diet (HFD)-induced fatty liver of mice and its mechanisms. Materials and Methods. Twenty-six male KM (Kunming) mice were divided into 3 groups, including the control group (n = 9), fed with normal diet; HFD group (n = 9), fed with HFD; ICAE + HFD group (n = 8), fed with HFD and administered with ICAE (3 g·kg−1·d−1) at the same time for 10 weeks. Body weight, liver weight, intra-abdominal and subcutaneous fat weight, serum triglyceride (TG), total cholesterol (TC), and blood glucose were determined to evaluate the preventive effects of ICAE on obesity. The average 24 h food consumption of the mice was monitored for 5 times in the 9th week of the experiment to investigate the effects of ICAE on food intake. Serum alanine transaminase (ALT) and aspartate aminotransferase (AST) were assayed to observe the influences of HFD and ICAE on liver function. HE staining was adopted to observe the influence of ICAE on the morphology of adipose tissue and liver tissue. Hepatic TG and TC content assay and oil red O staining were used to evaluate the influences of ICAE on HFD-induced fatty liver, and the protein expression of peroxisome proliferator-activated receptors γ (PPARγ) and adipose differentiation-related protein (ADRP) in liver were examined by immunoblotting. Results. ICAE treatment significantly reduced the increase of body weight, intra-abdominal, and subcutaneous fat and liver weight induced by HFD ( P < 0.001 ), but has no influence on food intake; ICAE treatment attenuated the elevation of serum TG, TC, and glucose, as well as serum ALT and AST ( P < 0.01 , P < 0.05 , P < 0.001 ) and dramatically decreased the content of TG in liver ( P < 0.01 ), but has no influence on hepatic TC content. HE staining and oil red O staining showed that ICAE significantly reduced HFD-induced white adipocyte hypertrophy and significantly inhibited lipid accumulation in liver. Immunoblotting showed that the protein levels of PPARγ and ADRP were significantly increased by HFD induction, which can be dramatically reduced by ICAE treatment ( P < 0.05 , P < 0.0001 ). Conclusion. ICAE has preventive effects on HFD-induced obesity and fatty liver in mice, exerted beneficial effects upon HFD-induced hepatic injury. The preventive effects of ICAE on fatty liver are concerned with the downregulation of PPARγ and ADRP protein expression in liver.

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Modulation of the gut microbiome and Firmicutes phylum reduction by a nutraceutical blend in the obesity mouse model and overweight humans: A double‐blind clinical trial

Nehmi‐Filho,

de Freitas,

Franco

et al. 2024

Food Science & Nutrition

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Overweight and obesity are closely linked to gut dysbiosis/dysmetabolism and disrupted De‐Ritis ratio [aspartate aminotransferase (AST)/alanine aminotransferase (ALT) ratio], which may contribute to chronic noncommunicable diseases onset. Concurrently, extensive research explores nutraceuticals, and health‐enhancing supplements, for disease prevention or treatment. Thus, sedentary overweight volunteers were double‐blind randomized into two groups: Novel Nutraceutical_(S) (without silymarin) and Novel Nutraceutical (with silymarin). Experimental formulations were orally administered twice daily over 180 consecutive days. We evaluated fecal gut microbiota, based on partial 16S rRNA sequences, biochemistry and endocrine markers, steatosis biomarker (AST/ALT ratio), and anthropometric parameters. Post‐supplementation, only the Novel Nutraceutical group reduced Clostridium clostridioforme (Firmicutes), Firmicutes/Bacteroidetes ratio (F/B ratio), and De‐Ritis ratio, while elevating Bacteroides caccae and Bacteroides uniformis (Bacteroidetes) in Brazilian sedentary overweight volunteers after 180 days. In summary, the results presented here allow us to suggest the gut microbiota as the action mechanism of the Novel Nutraceutical promoting metabolic hepatic recovery in obesity/overweight non‐drug interventions.

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A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice

Cited by 12 publications

References 59 publications

Modulation of Small-Intestine Morphology in Mice by a Novel Supplement Containing Silybum marianum, Yeast β-Glucan, Prebiotics, and Minerals

Modulation of Small-Intestine Morphology in Mice by a Novel Supplement Containing Silybum marianum, Yeast β-Glucan, Prebiotics, and Minerals

Preventive Effects of Ilex Cornuta Aqueous Extract on High-Fat Diet-Induced Fatty Liver of Mice

Modulation of the gut microbiome and Firmicutes phylum reduction by a nutraceutical blend in the obesity mouse model and overweight humans: A double‐blind clinical trial

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