A Novel Superfamily of Transporters for Allantoin and Other Oxo Derivatives of Nitrogen Heterocyclic Compounds in Arabidopsis

Desimone, Marcelo; Catoni, Elisabetta; Ludewig, Uwe; Hilpert, Melanie; Schneider, Anja; Kunze, Reinhard; Tegeder, Mechthild; Frommer, Wolf B.; Schumacher, Karin

doi:10.1105/tpc.010458

Cited by 100 publications

(117 citation statements)

References 26 publications

(25 reference statements)

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“…Characterized Arabidopsis PUP proteins transport adenine, cytosine or secondary compounds such as cytokinins and caffeine [8][9][10]. Two transporters in the Arabidopsis UPS family transport uracil, allantoin and the purines xanthine and hypoxanthine [11][12][13], and an UPS from French bean transports allantoin [14]. The remaining four nucleobase transporter families have orthologs in other prokaryotic and eukaryotic taxa.…”

Section: Introductionmentioning

confidence: 99%

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Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis

et al. 2012

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a b s t r a c tLocus At5g03555 encodes a nucleobase cation symporter 1 (AtNCS1) in the Arabidopsis genome. Arabidopsis insertion mutants, AtNcs1-1 and AtNcs1-3, were used for in planta toxic nucleobase analog growth studies and radio-labeled nucleobase uptake assays to characterize solute transport specificities. These results correlate with similar growth and uptake studies of AtNCS1 expressed in Saccharomyces cerevisiae. Both in planta and heterologous expression studies in yeast revealed a unique solute transport profile for AtNCS1 in moving adenine, guanine and uracil. This is in stark contrast to the canonical transport profiles determined for the well-characterized S. cerevisiae NCS1 proteins FUR4 (uracil transport) or FCY2 (adenine, guanine, and cytosine transport).

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Section: Introductionmentioning

confidence: 99%

“…Growth conditions were as previously described [19]. For the allantoin growth experiments, MAM was used in which 5 mM allantoin was the sole nitrogen source as previously described [11].…”

Section: Introductionmentioning

confidence: 99%

Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis

et al. 2012

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“…Of the 21 Arabidopsis PUPs several are known to transport adenine, cytosine or secondary compounds such as cytokinins and caffeine [14,15]. Two of the five transporters in the Arabidopsis UPS family transport uracil, allantoin and the purines xanthine and hypoxanthine [16,17]. Locus At5g03555 encodes a protein with significant amino acid similarity to FUR4, a uracil transporter of Saccharomyces cerevisiae -belonging to the purine-related transporter or nucleobase:cation symporter 1 family [18].…”

Section: Introductionmentioning

confidence: 99%

AtAzg1 and AtAzg2 comprise a novel family of purine transporters in Arabidopsis

2008

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a b s t r a c tIn plants, nucleobase biochemistry is highly compartmented relying upon a well-regulated and selective membrane transport system. In Arabidopsis two proteins, AtAzg1 and AtAzg2, show substantial amino acid sequence similarity to the adenine-guanine-hypoxanthine transporter AzgA of Aspergillus nidulans. Analysis of single and double mutant lines harboring T-DNA insertion alleles AtAzg1-1 and AtAzg2-1 reveal a marked resistance to growth in the presence of 8-azaadenine and 8-azaguanine but not to other toxic nucleobase analogues. Conversely, yeast strains expressing AtAzg1 and AtAzg2 gain heightened sensitivity to growth on 8-azaadenine and 8-azaguanine. Radio-labeled purine uptake experiments in yeast and in planta confirm the function of AtAzg1 and AtAzg2 as plant adenine-guanine transporters.

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“…In Arabidopsis, the genome contains loci that encode for five distinct nucleobase transporter families. Two nucleobase transporter families unique to plants include the twenty onemember purine permeases (PUP) that transport adenine, cytosine, and cytokinins (Gillissen et al 2000;Bürkle et al 2003;Cedzich et al 2008;Jelesko 2012;Szydlowski et al 2013) and the five-member ureide permeases (UPS) facilitating the movement of allantoin, uracil, uric acid, and xanthine (Desimone et al 2002;Schmidt et al 2004Schmidt et al , 2006. The remaining three nucleobase transporter families have orthologues among prokaryotes and eukaryotes.…”

Section: Introductionmentioning

confidence: 99%

The solute specificity profiles of nucleobase cation symporter 1 (NCS1) from Zea mays and Setaria viridis illustrate functional flexibility

et al. 2015

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The solute specificity profiles (transport and binding) for the nucleobase cation symporter 1 (NCS1) proteins, from the closely related C4 grasses Zea mays and Setaria viridis, differ from that of Arabidopsis thaliana and Chlamydomonas reinhardtii NCS1. Solute specificity profiles for NCS1 from Z. mays (ZmNCS1) and S. viridis (SvNCS1) were determined through heterologous complementation studies in NCS1-deficient Saccharomyces cerevisiae strains. The four Viridiplantae NCS1 proteins transport the purines adenine and guanine, but unlike the dicot and algal NCS1, grass NCS1 proteins fail to transport the pyrimidine uracil. Despite the high level of amino acid sequence similarity, ZmNCS1 and SvNCS1 display distinct solute transport and recognition profiles. SvNCS1 transports adenine, guanine, hypoxanthine, cytosine, and allantoin and competitively binds xanthine and uric acid. ZmNCS1 transports adenine, guanine, and cytosine and competitively binds, 5-fluorocytosine, hypoxanthine, xanthine, and uric acid. The differences in grass NCS1 profiles are due to a limited number of amino acid alterations. These amino acid residues do not correspond to amino acids essential for overall solute and cation binding or solute transport, as previously identified in bacterial and fungal NCS1, but rather may represent residues involved in subtle solute discrimination. The data presented here reveal that within Viridiplantae, NCS1 proteins transport a broad range of nucleobase compounds and that the solute specificity profile varies with species.

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A Novel Superfamily of Transporters for Allantoin and Other Oxo Derivatives of Nitrogen Heterocyclic Compounds in Arabidopsis

Cited by 100 publications

References 26 publications

Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis

Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis

AtAzg1 and AtAzg2 comprise a novel family of purine transporters in Arabidopsis

The solute specificity profiles of nucleobase cation symporter 1 (NCS1) from Zea mays and Setaria viridis illustrate functional flexibility

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