A Novel <sup>111</sup>In-Labeled Anti–Prostate-Specific Membrane Antigen Nanobody for Targeted SPECT/CT Imaging of Prostate Cancer

Chatalic, Kristell L.S.; Veldhoven-Zweistra, Joke; Bolkestein, Michiel; Hoeben, Sander; Koning, Gerben A.; Boerman, Otto C.; Jong, Marion de; Weerden, Wytske M. van

doi:10.2967/jnumed.115.156729

Cited by 106 publications

(105 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The early detection of nonpalpable tumors by TAB-ICG including hyperplasia forms proof of principle of the potential use of this antibody in the clinic in conjunction with other imaging modalities. For example, conjugated with radioactive imaging agents, TAB 004 could greatly improve the reliability of early breast cancer diagnosis when used in conjunction with computed tomography or MRI [39], [40], [41], [42], [43].…”

Section: Discussionmentioning

confidence: 99%

Antibody-Guided In Vivo Imaging for Early Detection of Mammary Gland Tumors

Moore

Roy

Zhou

et al. 2016

Translational Oncology

View full text Add to dashboard Cite

BACKGROUND: Earlier detection of transformed cells using target-specific imaging techniques holds great promise. We have developed TAB 004, a monoclonal antibody highly specific to a protein sequence accessible in the tumor form of MUC1 (tMUC1). We present data assessing both the specificity and sensitivity of TAB 004 in vitro and in genetically engineered mice in vivo. METHODS: Polyoma Middle T Antigen mice were crossed to the human MUC1.Tg mice to generate MMT mice. In MMT mice, mammary gland hyperplasia is observed between 6 and 10 weeks of age that progresses to ductal carcinoma in situ by 12 to 14 weeks and adenocarcinoma by 18 to 24 weeks. Approximately 40% of these mice develop metastasis to the lung and other organs with a tumor evolution that closely mimics human breast cancer progression. Tumor progression was monitored in MMT mice (from ages 8 to 22 weeks) by in vivo imaging following retro-orbital injections of the TAB 004 conjugated to indocyanine green (TAB-ICG). At euthanasia, mammary gland tumors and normal epithelial tissues were collected for further analyses. RESULTS: In vivo imaging following TAB-ICG injection permitted significantly earlier detection of tumors compared with physical examination. Furthermore, TAB-ICG administration in MMT mice enabled the detection of lung metastases while sparing recognition of normal epithelia. CONCLUSIONS: The data highlight the specificity and the sensitivity of the TAB 004 antibody in differentiating normal versus tumor form of MUC1 and its utility as a targeted imaging agent for early detection, tumor monitoring response, as well as potential clinical use for targeted drug delivery.

show abstract

Section: Discussionmentioning

confidence: 99%

Antibody-Guided In Vivo Imaging for Early Detection of Mammary Gland Tumors

Moore

Roy

Zhou

et al. 2016

Translational Oncology

View full text Add to dashboard Cite

show abstract

“…Another PSMAtargeting sdAb (JVZ-007) was labeled with 111 In for SPECT/ CT imaging. Excellent tumor targeting as early as 3 h after injection was observed (tumor-to-blood ratio of 48 6 5), in the absence of nonspecific uptake (14). Given the current success of clinical imaging using the available PSMAbinding peptides, the question remains of whether these sdAbs have enough added value to bring them to the clinic.…”

Section: Sdabs In Radionuclide Imagingmentioning

confidence: 99%

Same-Day Imaging Using Small Proteins: Clinical Experience and Translational Prospects in Oncology

Krasniqi¹,

D’Huyvetter²,

Devoogdt³

et al. 2018

J Nucl Med

104

View full text Add to dashboard Cite

Imaging of expression of therapeutic targets may enable stratification of patients for targeted treatments. The use of small radiolabeled probes based on the heavy-chain variable region of heavy-chain-only immunoglobulins or nonimmunoglobulin scaffolds permits rapid localization of radiotracers in tumors and rapid clearance from normal tissues. This makes high-contrast imaging possible on the day of injection. This mini review focuses on small proteins for radionuclide-based imaging that would allow same-day imaging, with the emphasis on clinical applications and promising preclinical developments within the field of oncology.

show abstract

“…These signals represent the presence of human class II MHC-positive cells and were consistently present in all BLT mice imaged. We also observed low nonspecific accumulation of signal in the kidneys and bladder, common VHH-elimination sites (23). PET/CT of untreated NOD/SCID mice (Figs.…”

Section: Noninvasive Immuno-pet Of Human Immune Reconstitutionmentioning

confidence: 90%

Noninvasive Imaging of Human Immune Responses in a Human Xenograft Model of Graft-Versus-Host Disease

et al. 2017

View full text Add to dashboard Cite

The immune system plays a crucial role in many diseases. Activation or suppression of immunity is often related to clinical outcome. Methods to explore the dynamics of immune responses are important to elucidate their role in conditions characterized by inflammation, such as infectious disease, cancer, or autoimmunity. Immuno-PET is a noninvasive method by which disease and immune cell infiltration can be explored simultaneously. Using radiolabeled antibodies or fragments derived from them, it is possible to image disease-specific antigens and immune cell subsets. Methods: We developed a method to noninvasively image human immune responses in a relevant humanized mouse model. We generated a camelid-derived single-domain antibody specific for human class II major histocompatibility complex products and used it to noninvasively image human immune cell reconstitution in nonobese diabetic severe combined immune deficiency g2/2 mice reconstituted with human fetal thymus, liver, and liver-derived hematopoietic stem cells (BLT mice). Results: We showed imaging of infiltrating immunocytes in BLT mice that spontaneously developed a graft-versus-host-like condition, characterized by alopecia and blepharitis. In diseased animals, we showed an increased PET signal in the liver, attributable to infiltration of activated class II major histocompatibility complex 1 T cells. Conclusion: Noninvasive imaging of immune infiltration and activation could thus be of importance for diagnosis and evaluation of treatment of graft-versus-host disease and holds promise for other diseases characterized by inflammation.

show abstract

A Novel ¹¹¹In-Labeled Anti–Prostate-Specific Membrane Antigen Nanobody for Targeted SPECT/CT Imaging of Prostate Cancer

Cited by 106 publications

References 28 publications

Antibody-Guided In Vivo Imaging for Early Detection of Mammary Gland Tumors

Antibody-Guided In Vivo Imaging for Early Detection of Mammary Gland Tumors

Same-Day Imaging Using Small Proteins: Clinical Experience and Translational Prospects in Oncology

Noninvasive Imaging of Human Immune Responses in a Human Xenograft Model of Graft-Versus-Host Disease

Contact Info

Product

Resources

About

A Novel 111In-Labeled Anti–Prostate-Specific Membrane Antigen Nanobody for Targeted SPECT/CT Imaging of Prostate Cancer

Cited by 106 publications

References 28 publications

Antibody-Guided In Vivo Imaging for Early Detection of Mammary Gland Tumors

Antibody-Guided In Vivo Imaging for Early Detection of Mammary Gland Tumors

Same-Day Imaging Using Small Proteins: Clinical Experience and Translational Prospects in Oncology

Noninvasive Imaging of Human Immune Responses in a Human Xenograft Model of Graft-Versus-Host Disease

Contact Info

Product

Resources

About

A Novel ¹¹¹In-Labeled Anti–Prostate-Specific Membrane Antigen Nanobody for Targeted SPECT/CT Imaging of Prostate Cancer