In this paper, we introduce a new subclass M λ,µ,ν Σ,q (γ, δ, ϕ) of analytic and bi-univalent functions involving a certain fractional integral operator which is defined based on quasi-subordination. For this class, we estimate the second and third coefficients of the Taylor-Maclaurin series expansions and upper bounds for Feketo-Szegö inequality. Furthermore, some relevant connections of certain special cases of the main results with those in several earlier works are also pointed out.