A novel stromal cell-dependent hematopoietic cell line established from temperature-sensitive SV40 T-antigen transgenic mice

Yanai, Nobuaki; Matsui, Naoko; Matsuda, Ken‐ichi; Furusawa, Tadashi; Okubo, Tadashi; Nakazawa, Tomoko; Ishibashi, Keiichiro; Nawa, Katsuhiko; Obinata, Masuo

doi:10.1016/s0301-472x(99)00027-2

Cited by 21 publications

(19 citation statements)

References 30 publications

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“…Culture of THS119 cells and TBR59 stromal cells was performed as reported previously (Yanai et al, 1999). Throughout the experiments, E-RDF medium (Kyokuto Pharmaceutical Co., Ltd., Tokyo, Japan) supplemented with 5% FBS, 10 mg/ml transferrin, 5 mg/ml insulin, 4.3 ng/ml sodium selenite, 1.53 mg/ml ethanolamine, and 100 mM 2-mercaptoethanol (2-ME) was used as a basic medium.…”

Section: Culture Of Ths119 Cells and The Cytokine-dependent Cell Linesmentioning

confidence: 99%

“…Using this stromal cell line as feeder layer, a novel stromal cell-dependent primitive hematopoietic cell line, THS119, was established from the sorted Lin -/Sca-1 + cells of bone marrow of ts SV40 large T-antigen gene transgenic mice after long-term passages (Yanai et al, 1999).…”

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confidence: 99%

“…We examined whether known cytokines could be replaced from the stromal cells for maintenance of THS119 cells, but none of the cytokines supported maintenance when IL-3, IL-6, IL-7, G-CSF, GM-CSF, M-CSF, Epo, or SCF was added to THS119 cells without stromal cells. However, when these cytokines were continuously added to the cocultures of THS119 cells with TBR59 cells for 2 months, the cells were able to grow in the presence of either IL-3 or IL-7 without TBR59 stromal cells and, finally, IL-3-(THS119/IL-3) or IL-7-(THS119/IL-7) dependent cell lines were established (Yanai et al, 1999).…”

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confidence: 99%

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Stromal Cells Provide Signals Different from Cytokines for STAT5 Activation in Hematopoietic Cells.

Sato

Yanai

Okubo

et al. 2001

Cell Struct. Funct.

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ABSTRACT. After detachment from the stromal cells, hematopoietic stem cells are thought to differentiate to the cytokine-dependent stages where their growth and differentiation are promoted by these cytokines. To examine the stromal regulation of hematopoietic stem cells, we previously established a primitive hematopoietic stem-like cell line, THS119, whose growth was dependent on the bone marrow stromal cell line, TBR59, and from which IL-3-(THS119/IL-3) or IL-7-(THS119/IL-7) dependent cell lines were then generated. Using these cell lines, we examined the difference in signals mediated by the stromal cells and cytokines.The cytokine-dependent cell lines (THS119/IL-3 and THS119/IL-7) showed induction of STAT5 phosphorylation and target genes for STAT5 such as CIS, pim-1, p21 and bcl-xL upon addition of IL-3 or IL-7. IL-3 or IL-7 also induced STAT5 phosphorylation and STAT5 target genes of the stromal cell-dependent cell line, THS119, in the absence of stromal cells at levels similar to the cytokine-dependent cell lines. However, quite interestingly, TBR59 stromal cells could not induce STAT5 phosphorylation of THS119 cells, although they did induce STAT5 target genes in THS119 cells. In addition, the mRNAs for STAT5 target genes in THS119 cells on the stromal cells seemed to be more stable than those in the cytokine-dependent cell lines. Expression of the antiapoptotic genes bcl-2 and bcl-xL was higher in the stromal cell-dependent cell line than in the cytokine-dependent cell lines. These results suggested that stromal cells and cytokines may provide different signals for growth and differentiation of the hematopoietic cells.

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Section: Culture Of Ths119 Cells and The Cytokine-dependent Cell Linesmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Stromal Cells Provide Signals Different from Cytokines for STAT5 Activation in Hematopoietic Cells.

Sato

Yanai

Okubo

et al. 2001

Cell Struct. Funct.

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“…Stromal cell-dependent hematopoietic stem-like cell line (THS-119) (21) Lin-c-Kit + Sca1 + CD34low +, hematopoietic cell specific transcription factors…”

Section: Hematopoietic Cell Linesmentioning

confidence: 99%

“…We established several stroma-dependent hematopoietic cell lines to examine the regulatory mechanisms of hematopoiesis by stromal cells. (20)(21)(22)(23)(24) Among them, a primitive hematopoietic cell line, THS119, was established from BM Lin -/Sca-1 + cells of the transgenic mice after lengthy passaging by coculture with TBR59 (21) THS119 cells exhibited immature primitive hematopoietic cells such as forming cobblestones and surface phenotypes (Lin -/c-Kit + /Sca1 + ), and multiple expressions of both earlier developmental markers of myeloid, lymphoid and the hematopoietic cell-specific transcription factors. Growth of THS119 cells is ts T-antigen-dependent and stromal celldependent.…”

Section: The Blood-cerebrospinal Fluid (Bcsfb) Barriermentioning

confidence: 99%

The immortalized cell lines with differentiation potentials: Their establishment and possible application

Obinata

2007

Cancer Science

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A novel stromal cell-dependent hematopoietic cell line established from temperature-sensitive SV40 T-antigen transgenic mice

Cited by 21 publications

References 30 publications

Stromal Cells Provide Signals Different from Cytokines for STAT5 Activation in Hematopoietic Cells.

Stromal Cells Provide Signals Different from Cytokines for STAT5 Activation in Hematopoietic Cells.

The immortalized cell lines with differentiation potentials: Their establishment and possible application

Possible Applications of Conditionally Immortalized Tissue Cell Lines with Differentiation Functions

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