A Novel Strategy to Enhance Mesenchymal Stem Cell Migration Capacity and Promote Tissue Repair in an Injury Specific Fashion

Abstract

Mesenchymal stem cells (MSCs) of bone marrow origin appear to be an attractive candidate for cell-based therapies. However, the major barrier to the effective implementation of MSC-based therapies is the lack of specific homing of exogenously infused cells and overall the inability to drive them to the diseased or damaged tissue. In order to circumvent these limitations, we developed a preconditioning strategy to optimize MSC migration efficiency and potentiate their beneficial effect at the site of injury. Initially, we screened different molecules by using an in vitro injury-migration setting, and subsequently, we evaluated the effectiveness of the different strategies in mice with acute kidney injury (AKI). Our results showed that preconditioning of MSCs with IGF-1 before infusion improved cell migration capacity and restored normal renal function after AKI. The present study demonstrates that promoting migration of MSCs could increase their therapeutic potential and indicates a new therapeutic paradigm for organ repair.

Cell Projections and Extracellular Matrix Cross the Interstitial Interface within the Renal Stem/Progenitor Cell Niche: Accidental, Structural or Functional Cues?

“…Since revealing data are missing until now, the panel of speculations is broad. Possible reasons are that an appropriate type of stem/progenitor cells is not yet available, transfer from the beneficial culture environment into diseased parenchyma damages implanted cells or influences of interstitial fluid and degrading extracellular matrix (ECM) prevent regeneration [10]. …”

Bone Marrow–Derived Mononuclear Cell Therapy Accelerates Renal Ischemia-Reperfusion Injury Recovery by Modulating Inflammatory, Antioxidant and Apoptotic Related Molecules

“…One of the characteristics of stem cells is their ability to migrate to the injured tissue as a result of inflammatory signaling [25]. To track the migration of BMMCs and their location in different periods until the end of the experimental protocol, 99m Tc-BMMCs in the S-C and IR-C groups were evaluated 2 and 4 h after infusion.…”

Testis peritubular myoid cells increase their motility and express matrix‐metalloproteinase 9 (MMP‐9) after interaction with embryonal carcinoma cells

“…For example, a variety of cytokines and growth factors, such as insulin-like growth factor-1 (IGF-I), glial cell-derived neurotrophic factor or different interleukins (IL-6 or IL-8) have been described as molecules that stimulate migration behavior in mesenchymal stem cells and their effect on PTCs would give a hint to elucidate the molecules implicated in EC cells and PTC interactions (Yew et al, 2011;Xinaris et al, 2013).…”