A novel strategy of engineering genetically encoded probe for ultrasensitive sensing Hg2+ with unusual planar trigonometric coordination configuration

Genetically encoded protein sensors for metal ion detection in biological systems: a review and bibliometric analysis

“…After binding with Hg 2+ , the sensor as a whole shows a special planar triangular ligand configuration, which is able to cause enough conformational changes to obtain stronger fluorescence, and the excellent performance of MerBD itself also endows the probe with excellent sensitivity and selectivity. 118 The creation of sfGFP-MerBD provides a solution to the poor performance of natural metalloproteins and is an effective way to develop more natural metal-binding proteins with excellent properties.…”