A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds

Abstract

The main aim of this study was to evaluate the oils from chamomile seeds as a new source of bioactive compounds suitable for human consumption. A green extraction technique with supercritical carbon dioxide (sc-CO 2 ) at pressures up to 450 bar and temperatures up to 60 °C was employed for the production of a high amount of biologically active oil. Additionally, exhausted waste material was re-extracted using sc-CO 2 with the addition of ethanol. By optimization in operating pressure, temperature, production cost, fraction of milled seeds, and co-solvent addition, the amount of separated chamomile oil increased from 2.4 to 18.6% and the content of unsaturated fatty acids up to 88.7%. Oils contained α-bisabolol oxide A and B in amounts up to 1.4%. Linoleic acid was detected in an amount up to 711.1 mg/g and α-linolenic acid up to 27.5 mg/g. The total phenolic content in separated oil reached 80.4 mg GAE/g while the total flavonoid content reached 11.6 mg QE/g. The obtained chamomile oils showed antioxidant activity with an IC 50 of up to 3.9 mg/mL. Among the 23 tested microorganisms, the antimicrobial activity of oils was the most pronounced against Gram-positive bacteria. The cytotoxic activity of oils was tested on normal and cancer-derived cell lines. Results indicated a significant potential for oil from chamomile seeds, produced in an eco-friendly manner, as a functional food. Supplementary Information The online version contains supplementary material available at 10.1007/s11947-023-03038-9.

Extraction and Separation of Natural Products from Microalgae and Other Natural Sources Using Liquefied Dimethyl Ether, a Green Solvent: A Review

“…The use of scCO 2 remarkably improves the extraction efficiency of functional components, enabling selective extraction via temperature or pressure control [41][42][43]. The extraction of functional components using scCO 2 has been applied to various natural food sources, such as essential oils [44], γ-oryzanol [45], chamomile seed oil [46], and hops [47], as well as for caffeine removal [48] and oil extraction from microalgae [49][50][51]. However, the extraction of functional components from highly aqueous samples using supercritical methods is challenging [52].…”