A novel strategy for creating a new system of third‐generation hybrid rice technology using a cytoplasmic sterility gene and a genic male‐sterile gene

Song, Shufeng; Wang, Tiankang; Li, Yixing; Hu, Jun; Kan, Ruifeng; Qiu, Mudan; Deng, Yingde; Liu, Peixun; Zhang, Licheng; Dong, Hao; Li, Chengxia; Yu, Dong; Li, Xin-Qi; Yuan, Dingyang; Yuan, Longping; Li, Li

doi:10.1111/pbi.13457

Cited by 50 publications

(43 citation statements)

References 38 publications

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“…Self-pollination of the transgenic plant produced 50% male sterile seeds and 50% transgenic seeds that could be sorted out based on the red uorescence. Since then, various systems similar to SPT have been constructed in maize and rice with different NMS genes and pollen-killer genes, but all use RFP as the seed sorting marker (Chang et RFP-based seed sorting requires machines of high precision and complicated designs for seed delivery, orescence excision, orescence detection, image acquisition, and seed sorting (Wu et al, 2016;Song et al, 2021). However, partially lled seeds or pathogen-infected seeds often have low orescence that cannot be detected by the machine, resulting in contamination of the male sterile seeds by the maintainer seeds.…”

Section: Resultsmentioning

confidence: 99%

Construction of a Weight-based Seed Sorting System for the Third-generation Hybrid Rice

Qiu

Wang

et al. 2021

Preprint

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Background: The third-generation hybrid rice technology can be constructed by transforming a recessive nuclear male sterile (NMS) mutant with a transgenic cassette containing three functional modules: the wild type male fertility gene to restore the fertility of the mutant, the pollen killer gene that specifically kills the pollen grains carrying the transgene, and the red fluorescence protein (RFP) gene to mark the transgenic seed (maintainer). The transgenic plant produces 1:1 NMS seeds and maintainer seeds that can be distinguished by the RFP signal. However, the RFP signals in the partially filled or pathogen-infected maintainer seeds are often too weak to be detected by RFP-based seed sorting machine, resulting in intermingling of the maintainer seeds with NMS seeds.Results: Here we constructed a weight-based seed sorting system for the third-generation hybrid rice technology by silencing the genes encoding ADP-glucose pyrophosphorylase (AGP) essential for endosperm starch biosynthesis via endosperm-specific expression of artificial microRNAs (amiRNAs). In this system, the NMS seeds have normal endosperm and are heavy, but the maintainer seeds have shrunken endosperms and are light-weighted. The maintainer seeds can be easily and accurately sorted out from the NMS seeds by weight-sorting machines, so pure and fully filled NMS seeds are available.Conclusions: The weight-based seed sorting system shows obvious advantages over the RFP-based seed sorting system in accuracy, efficiency, and cost for propagation of pure male sterile seeds. These characteristics will significantly increase the value and transgenic safety of the third-generation hybrid rice technology.

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Section: Resultsmentioning

confidence: 99%

Construction of a Weight-based Seed Sorting System for the Third-generation Hybrid Rice

Qiu

Wang

et al. 2021

Preprint

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“…In order to circumvent these limitations, a novel SPT-based technique was established to develop a third generation hybrid (Song et al, 2020). The rice male fertility gene CYP703A3 encodes a cytochrome P450 hydroxylase and is critical for pollen development.…”

Section: Seed Production Technology For Next-generation Hybrid Productionmentioning

confidence: 99%

“…The selfing process of this maintainer line produced both transgenic (fertile) and non-transgenic (sterile) seeds at a 1:1 ratio. The crossing of sterile lines with other elite cultivars (male) produced hybrids with a 13% increase in yield compared to the parental lines (Song et al, 2020). Hence, the application of a CMS gene successfully created a transgene-free plant, outperforming the hybrid plant that is entirely compatible with the "zero tolerance" policy of genetically modified organisms (GMOs) production.…”

Section: Seed Production Technology For Next-generation Hybrid Productionmentioning

confidence: 99%

Exploiting Genic Male Sterility in Rice: From Molecular Dissection to Breeding Applications

et al. 2021

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Rice (Oryza sativa L.) occupies a very salient and indispensable status among cereal crops, as its vast production is used to feed nearly half of the world’s population. Male sterile plants are the fundamental breeding materials needed for specific propagation in order to meet the elevated current food demands. The development of the rice varieties with desired traits has become the ultimate need of the time. Genic male sterility is a predominant system that is vastly deployed and exploited for crop improvement. Hence, the identification of new genetic elements and the cognizance of the underlying regulatory networks affecting male sterility in rice are crucial to harness heterosis and ensure global food security. Over the years, a variety of genomics studies have uncovered numerous mechanisms regulating male sterility in rice, which provided a deeper and wider understanding on the complex molecular basis of anther and pollen development. The recent advances in genomics and the emergence of multiple biotechnological methods have revolutionized the field of rice breeding. In this review, we have briefly documented the recent evolution, exploration, and exploitation of genic male sterility to the improvement of rice crop production. Furthermore, this review describes future perspectives with focus on state-of-the-art developments in the engineering of male sterility to overcome issues associated with male sterility-mediated rice breeding to address the current challenges. Finally, we provide our perspectives on diversified studies regarding the identification and characterization of genic male sterility genes, the development of new biotechnology-based male sterility systems, and their integrated applications for hybrid rice breeding.

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“…It is helpful to elucidate the mechanism of pollen development. Gametophytic male sterility plays an important role in the male sterility seed production system of three-generation hybrid rice, in which the pollen lethal elements are gametophytic male sterility [33][34][35][36]. Therefore, the preparation of GMS also has key value in providing the new pollen lethal element.…”

Section: Construction and Veri Cation Of A Gms Preparation And Identi Cation Systemmentioning

confidence: 99%

Development of an Efficient Preparation and Verifying System for Rice Gametophytic Male Sterility using RNAi on Candidate Gene and OsMYB86R as a Reporter

Chen

Zhu

Shi

et al. 2021

Preprint

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Background: Gametophytic male sterility plays an important role in the study of pollen development mechanism and the breeding of three-generation hybrid rice. Because of the inherent phenotypic and genetic characteristics of gametophytic male sterility, it is very hard to find and identify gametophytic male sterility, which makes the related research lay behind the sporophytic male sterility. On the other hand, with the abundance of gene transcription data, a large number of pollen-specific genes have been found, and most of them are possibly associated with gametophytic male sterility. In order to promote the research of these genes in pollen development and heterosis utilization, it is necessary to construct an efficient, economical and accurate method to test these genes and identify if they are gametophytic male sterility related genes.Results: In this study, at first, the OsC1/OsMYB86 gene involved in anthocyanin synthesis in rice was modified and identified, and the function of revised OsMYB86R gene was created as the same as OsMYB86, and OsMYB86R is more convenient to be used as a reporter gene because the restriction site is removed. Then, an ascorbic acid oxidase gene OsPTD1 was downregulated using RNAi driven by its own promoter, which resulted in abnormal pollen tube growth; Lastly, the RNAi elements were linked with OsMYB86R and together-transferred into an osmyb86 mutant, and the purple color segregation of T1 and F1 derived from positive plants were distorted, which showed that the OsPTD1 related gametogenic male sterility was successfully prepared and determined.Conclusion: In this study, an easy and efficient method to prepare and identify gametophyte male sterility was established by using the strategy of RNAi and OsMYB86R as reporter. Comparing with the current methods, the advantages of this method are: (1) in material preparation, save time, a generation less comparing with conventional method, also a lot of mutation screening maybe avoided; (2) in identification, less cost, no PCR, electrophoresis, and other processes needed, and no expensive chemicals and instruments required; (3) in the results, more accurate, much less background affection, no damage on the plant. As a result, it is an easy, efficient, low-cost, accurate method to prepare and identify gametophytic male sterility genes. It is very important in elucidating the mechanism of pollen development and promoting the utilization of heterosis.

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A novel strategy for creating a new system of third‐generation hybrid rice technology using a cytoplasmic sterility gene and a genic male‐sterile gene

Cited by 50 publications

References 38 publications

Construction of a Weight-based Seed Sorting System for the Third-generation Hybrid Rice

Construction of a Weight-based Seed Sorting System for the Third-generation Hybrid Rice

Exploiting Genic Male Sterility in Rice: From Molecular Dissection to Breeding Applications

Development of an Efficient Preparation and Verifying System for Rice Gametophytic Male Sterility using RNAi on Candidate Gene and OsMYB86R as a Reporter

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