A novel strain of endophytic Streptomyces for the biocontrol of strawberry anthracnose caused by Glomerella cingulata

Marian, Malek; Ohno, Teppei; Suzuki, Hirofumi; Kitamura, Hatsuyoshi; Kuroda, Katsutoshi; Shimizu, Mitsuaki

doi:10.1016/j.micres.2020.126428

Cited by 35 publications

(19 citation statements)

References 56 publications

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“… Streptomyces sp. MBFA-172 was capable of suppressing strawberry anthracnose caused by Glomerella cingulata , and thus would contribute to the development of a practical biocontrol approach [68]. Streptomyces violaceusniger AC12AB could provide a dual benefit by decreasing potato common scab disease severity and increasing potato yield as an effective alternative strategy to manage this disease [69].…”

Section: Discussionmentioning

confidence: 99%

Streptomyces botrytidirepellens sp. nov., a novel actinomycete with antifungal activity against Botrytis cinerea

Jiang

Song

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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The fungal pathogen Botrytis cinerea is the causal agent of devastating gray mold diseases in many economically important fruits, vegetables, and flowers, leading to serious economic losses worldwide. In this study, a novel actinomycete NEAU-LD23T exhibiting antifungal activity against B. cinerea was isolated, and its taxonomic position was evaluated using a polyphasic approach. Based on the genotypic, phenotypic and chemotaxonomic data, it is concluded that the strain represents a novel species within the genus Streptomyces , for which the name Streptomyces botrytidirepellens sp. nov. is proposed. The type strain is NEAU-LD23T (=CCTCC AA 2019029T=DSM 109824T). In addition, strain NEAU-LD23T showed a strong antagonistic effect against B. cinerea (82.6±2.5%) and varying degrees of inhibition on nine other phytopathogenic fungi. Both cell-free filtrate and methanol extract of mycelia of strain NEAU-LD23T significantly inhibited mycelial growth of B. cinerea. To preliminarily explore the antifungal mechanisms, the genome of strain NEAU-LD23T was sequenced and analyzed. AntiSMASH analysis led to the identification of several gene clusters responsible for the biosynthesis of bioactive secondary metabolites with antifungal activity, including 9-methylstreptimidone, echosides, anisomycin, coelichelin and desferrioxamine B. Overall, this research provided us an excellent strain with considerable potential to use for biological control of tomato gray mold.

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Section: Discussionmentioning

confidence: 99%

Streptomyces botrytidirepellens sp. nov., a novel actinomycete with antifungal activity against Botrytis cinerea

Jiang

Song

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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“…Moreover, some biocontrol agents have plant growth promotion abilities. Several studies reported that beneficial fungi and bacteria could effectively control or reduce strawberry diseases [12,[31][32][33]. However, this area is still in its infancy and much of the work is limited to the lab and the greenhouse.…”

Section: Discussionmentioning

confidence: 99%

A Potential Application of Endophytic Bacteria in Strawberry Production

et al. 2021

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Endophytic bacteria could be used as a tool to promote plant growth, enhance abiotic stress tolerance, and inhibit plant pathogen growth. More than 100 bacteria, previously tested to have biocontrol activity against fungal pathogens from our lab, were chosen for pathogen inhibition in vitro with antibiosis assays against strawberry specific pathogens. Three potential endophytic bacteria were tested in the greenhouse for disease reduction and growth performance. Finally, field trials were conducted for fruit yield production. Our results showed that three endophytic bacteria significantly inhibited the growth of the strawberry pathogen Colletotrichum gloeosporioides from antibiosis assays and were identified as Bacillus velezensis strains IALR308, IALR585, and IALR619. Inoculation of these bacteria significantly reduced strawberry disease in greenhouse conditions with pathogen infection. Field trials showed that IALR619 has potential to influence marketable fruit yield when strawberry plants were inoculated twice over the growing season. All three bacteria had the ability to produce auxin and to solubilize phosphate. The antibiotics surfactin and iturin were also detected in IALR585 and IALR619. In conclusion, Bacillus velezensis IALR619 has potential inhibition of strawberry pathogen growth in the greenhouse and possible ability to increase marketable fruit yield in the field.

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“…The study of endophytes has emerged as an alternative method to control vascular wilting diseases [ 27 ]. Endophytes have been shown to stimulate plant growth [ 28 – 30 ] in addition to inducing biotic stress resistance [ 31 ], suppressing disease [ 32 ], and effectively competing for the space available for pathogens [ 13 ]. The potential of bacterial endophytes to inhibit different fungal and bacterial plant pathogens has been reported [ 33 – 35 ].…”

Section: Introductionmentioning

confidence: 99%

Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum

et al. 2021

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Background The native potatoes (Solanum tuberosum subsp. tuberosum L.) grown in Chile (Chiloé) represent a new, unexplored source of endophytes to find potential biological control agents for the prevention of bacterial diseases, like blackleg and soft rot, in potato crops. Result The objective of this study was the selection of endophytic actinobacteria from native potatoes for antagonistic activity against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum, and their potential to suppress tissue maceration symptoms in potato tubers. This potential was determined through the quorum quenching activity using a Chromobacterium violaceaum ATCC 12472 Wild type (WT) bioassay and its colonization behavior of the potato plant root system (S. tuberosum) by means of the Double labeling of oligonucleotide probes for fluorescence in situ hybridization (DOPE-FISH) targeting technique. The results showed that although Streptomyces sp. TP199 and Streptomyces sp. A2R31 were able to inhibit the growth of the pathogens, only the Streptomyces sp. TP199 isolate inhibited Pectobacterium sp. growth and diminished tissue maceration in tubers (p ≤ 0.05). Streptomyces sp. TP199 had metal-dependent acyl homoserine lactones (AHL) quorum quenching activity in vitro and was able to colonize the root endosphere 10 days after inoculation. Conclusions We concluded that native potatoes from southern Chile possess endophyte actinobacteria that are potential agents for the disease management of soft rot and blackleg.

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A novel strain of endophytic Streptomyces for the biocontrol of strawberry anthracnose caused by Glomerella cingulata

Cited by 35 publications

References 56 publications

Streptomyces botrytidirepellens sp. nov., a novel actinomycete with antifungal activity against Botrytis cinerea

Streptomyces botrytidirepellens sp. nov., a novel actinomycete with antifungal activity against Botrytis cinerea

A Potential Application of Endophytic Bacteria in Strawberry Production

Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum

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