A novel sterol from Chinese truffles Tuber indicum

Gao, Jing; Hu, Lin; Liu, Ji‐Kai

doi:10.1016/s0039-128x(01)00105-2

Cited by 97 publications

(30 citation statements)

References 12 publications

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“…Since, this compound has been previously isolated from Alternaria alternata (Seitz & Paukstelis, 1977), Pleurotus ostreatus (Chobot et al, 1997) and Tuber indicum (Jinming et al, 2001) our spectroscopic data (not presented) were compared with those reported in the literature, confirming the identification. Although this steroid is a common constituent of fungus biomembranes (Jinming et al, 2001), to the best of our knowledge, this is the first report describing its phytotoxicity. The results of the ATP bioassay showed that the phytotoxicity observed for this fungus may be explained in part for the presence of this steroid.…”

Section: Effects Of Corynespora Cassiicola On Lantana Camarasupporting

confidence: 73%

Effects of Corynespora cassiicola on Lantana camara

et al. 2010

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-The present study combines the examination of toxins produced by C. cassiicola and the effects of the fungus colonization on L. camara. C. cassiicola was cultivated on solid media and the crude extracts CAE and CE were produced. Both extracts were submitted to a seed germination and growth assay utilizing Physalis ixocarpa, Trifolium alexandrinum, Lolium multiflorum and Amaranthus hypochodriacus. The effect of the extracts on the ATP-synthesis in isolated spinach chloroplasts was also tested. Bioassay guided chromatographic fractionation identified the most active extract (CAE). From this extract ergosta-4,6,8(14),22-tetraen-3-one (C1) and fatty acids were isolated. The C1 compound reduce ATP synthesis in isolated spinach chloroplasts. The interference of fatty acids with ATP synthesis and also with weed growth provides one explanation of the phytogrowth-inhibitory properties of such fungal extracts. Histological observations involving fungus-plant interaction were made on L. camara plants inoculated with C. cassiicola conidia suspension. After inoculations, fragments of the leaf blades were prepared for observation by light and scanning electron microscopy. Fungal colonization of Lantana camara was typical of a necrotroph and penetration initiated a hypersensitive response. L. camara reacted to the pathogen penetration through thickening of the epidermis walls, cytoplasm granulation and a cicatrisation tissue.Keywords: allelopathy, bioassays, photosynthesis inhibitor, phytotoxicity.RESUMO -O presente estudo combina a investigação de toxinas produzidas por C. cassiicola e os efeitos da colonização do fungo sobre L. camara. C. cassiicola foi cultivado em meio sólido do qual se obtiveram os extratos brutos CAE e CE. Ambos os extratos foram submetidos aos testes de germinação e crescimento utilizando Physalis ixocarpa, Trifolium alexandrinum, Lolium multiflorum e Amaranthus hypochodriacus e sobre a síntese de ATP em cloroplastos isolados de espinafre. Os bioensaios direcionaram o fracionamento cromatográfico permitindo a identificação do extrato mais ativo (CAE). Desse extrato isolou-se o composto ergosta-4,6,8(14),22-tetraen-3-ona (C1) e ácidos graxos. O composto C1 reduz a síntese de ATP em cloroplasto isolados de espinafre. A interferência dos ácidos graxos sobre a síntese de ATP e crescimento das plantas daninhas fornece uma explicação para as propriedades fitoinibitórias dos extratos fúngicos. Realizaram-se observações histológicas envolvendo a interação fungo-planta em plantas de Lantana camara inoculadas com suspensão de conídios de C. cassiicola. Após as inoculações, fragmentos da lâmina foliar foram preparados para observações por microscopia de luz e eletrônica de varredura. A colonização fúngica de L. camara foi tipicamente necotrófica e a penetração iniciou uma resposta hipersensível. L. camara reagiu à penetração do patógeno pelo espessamento da parede da epiderme, granulação do citoplasma e tecido de cicatrização.Palavras-chave: alelopatia, bioensaio, inibidor da fotossíntese, fitotoxicidade...

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Section: Effects Of Corynespora Cassiicola On Lantana Camarasupporting

confidence: 73%

Effects of Corynespora cassiicola on Lantana camara

et al. 2010

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“…Truffles, a group of hypogenous mushroom belonging to the Ascomycota, were particularly appreciated for their unique aroma, great economic value and potential health benefits (Hall et al 2003; Beara et al 2014). In fact, truffles were rich in unsaturated fatty acids (UFA), some kind of free sugars that are good for human health, and therapeutic compounds with anti-inflammatory, antioxidant, antimicrobial, anti-mutagenic and anti-carcinogenic properties (Bokhary and Sarwat 1993; Carneiro et al 2013; Culleré et al 2010; Dundar et al 2012; Gao et al 2001a, b; Murcia et al 2002; Saltarelli et al 2008; Sawaya et al 1985; Stojković et al 2013). …”

Section: Introductionmentioning

confidence: 99%

Nutritional value, chemical composition and antioxidant activity of three Tuber species from China

et al. 2017

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Nutritional value, chemical composition and antioxidant activity of the traditional edible truffles Tuber latisporum, T. subglobosum and T. pseudohimalayense, from China were evaluated. Powder formulations of the three truffles revealed the presence of essential nutrients, such as proteins, carbohydrates and unsaturated fatty acids, and T. latisporum presented the highest contents of total sugar (50.10 g/100 g) and monounsaturated fatty acids (265.19 mg/100 g dw); T. pseudohimalayense showed the highest content of polyunsaturated fatty acids (367.98 mg/100 g dw). They all presented a low fat content but high contents of proteins and unsaturated fatty acid, which is beneficial to human health. The methanol extract from T. pseudohimalayense showed a high radicals scavenging activity and the highest content of total phenols (735.01 mg/100 g dw); T. subglobosum presented the highest content of flavonoids (1355.43 mg/100 g dw). All these extracts could be used as potential antioxidant sources to prevent diseases related to oxidative damage.

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“…In our assay condition, the methylene chloride extract of M. esculenta showed the highest antioxidant activity. Phytochemical investigation of methylene chloride extract led to the isolation of five bioactive constituents (Figure 1), four fungal sterols: 5-dihydroergosterol (1) (Keller, Maillard, & Hostettmann, 1996), ergosterol peroxide (2) (Yue, Chen, Lin, & Sun, 2001), ergosterol (3) (Zhang, Mills, & Nair, 2002), cerevisterol (4) (Jinming, Lin, & Jikai, 2001), and trilinolein (5) (Ramsewak, Nair, Murugesan, Mattson, & Iasada, 2001). Methyl myristate (6), 1-linoleoylglycerol (7), and ceramide (8) were also isolated and identified by spectroscopic ( 1 H-and 13 C-NMR, MS) data by comparison with literature values (Iriko et al, 2002).…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and NF-κB inhibitory constituents isolated fromMorchella esculenta

Kim

Lau

Tay

et al. 2011

Natural Product Research

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Morchella esculenta Pers. (Morchellaceae) was investigated for its antioxidant activity by measuring the intracellular reactive oxygen species in HT-29 colon cancer cells. The methylene chloride extract, which showed the highest antioxidant activity, led to the isolation of four fungal sterols, 1-4, and trilinolein (5), in addition to methyl myristate (6), 1-linoleoylglycerol (7), and ceramide (8). The isolated compounds were identified through the analysis of various spectroscopic methods. In the hydroxyl radical assay, 5-dihydroergosterol exhibited significant antioxidant activity. All compounds isolated were also tested using an enzyme-based Elisa NF-κB assay. Fungal sterols and trilinolein showed significant inhibition of NF-κB activation in the NF-κB assay.

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A novel sterol from Chinese truffles Tuber indicum

Cited by 97 publications

References 12 publications

Effects of Corynespora cassiicola on Lantana camara

Effects of Corynespora cassiicola on Lantana camara

Nutritional value, chemical composition and antioxidant activity of three Tuber species from China

Antioxidant and NF-κB inhibitory constituents isolated fromMorchella esculenta

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