A novel steroidal saponin glycoside from Fagonia indica induces cell-selective apoptosis or necrosis in cancer cells

Waheed, Abdul; Barker, James; Barton, S.K.; Owen, Caroline P.; Ahmed, Sabbir; Carew, Mark

doi:10.1016/j.ejps.2012.07.004

Cited by 60 publications

(37 citation statements)

References 31 publications

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“…Anticarcinogenic activity was shown by a variety of steroidal saponins from different plants including Digitalis trojana (Kirmizibekmez et al 2014), Allium schoenoprasum (Timité et al 2013), Dioscorea zingiberensis (Tong et al 2012), Fagonia indica (Waheed et al 2012), Rhizoma Paridis (Xiao et al 2012), Solanum violaceum (Yen et al 2012), Agave sisalana (Chen et al 2011a, b), Anemarrhena asphodeloides (Kang et al 2011), Dioscorea bulbifera , Trigonella foenum-graecum (Kawabata et al 2011), Paris polyphylla (Zhu et al 2011), Raphia farinifera (Tapondjou et al 2015) etc.…”

Section: The Value Of Steroidal Saponinsmentioning

confidence: 99%

Recent advances in steroidal saponins biosynthesis and in vitro production

Upadhyay

Jeena

Shikha

et al. 2018

Planta

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Main conclusion Steroidal saponins exhibited numerous pharmacological activities due to the modification of their backbone by different cytochrome P450s (P450) and UDP glycosyltransferases (UGTs). Plant-derived steroidal saponins are not sufficient for utilizing them for commercial purpose so in vitro production of saponin by tissue culture, root culture, embryo culture, etc, is necessary for its large-scale production.Saponin glycosides are the important class of plant secondary metabolites, which consists of either steroidal or terpenoidal backbone. Due to the existence of a wide range of medicinal properties, saponin glycosides are pharmacologically very important. This review is focused on important medicinal properties of steroidal saponin, its occurrence, and biosynthesis. In addition to this, some recently identified plants containing steroidal saponins in different parts were summarized. The high throughput transcriptome sequencing approach elaborates our understanding related to the secondary metabolic pathway and its regulation even in the absence of adequate genomic information of non-model plants. The aim of this review is to encapsulate the information related to applications of steroidal saponin and its biosynthetic enzymes specially P450s and UGTs that are involved at later stage modifications of saponin backbone. Lastly, we discussed the in vitro production of steroidal saponin as the plant-based production of saponin is time-consuming and yield a limited amount of saponins. A large amount of plant material has been used to increase the production of steroidal saponin by employing in vitro culture technique, which has received a lot of attention in past two decades and provides a way to conserve medicinal plants as well as to escape them for being endangered.

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Section: The Value Of Steroidal Saponinsmentioning

confidence: 99%

Recent advances in steroidal saponins biosynthesis and in vitro production

Upadhyay

Jeena

Shikha

et al. 2018

Planta

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“…It is found in sub-tropical regions and is known for its medicinal value [28]. Fagonia indica is a small spiny shrub with reputed medicinal value and is distributed in the warm and dried areas of the middle east and the Indian subcontinent [29,30]. Calendula arvensis, commonly known as Field Marigold, is an annual herb, which is naturally distributed in Southern Europe, Iran, Afghanistan and Pakistan and is known for various ethnic medicinal properties [31,32].…”

Section: Discussionmentioning

confidence: 99%

Bioactivity-driven high throughput screening of microbiomes of medicinal plants for discovering new biological control agents

Iqrar¹,

Elsayed

2019

Preprint

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In a preliminary DNA-based microbiome studies, diverse culturable and unculturable bacterial taxa were identified in the roots and rhizospheres of different medicinal plants. In this report, culturable endophytic bacteria were isolated from four economically important medicinal plants Dodonaea viscosa, Fagonia indica, Caralluma tuberculata and Calendula arvensis. On the basis of initial antimicrobial screening, nine bacterial species in seven different genera, Streptomyces, Pseudomonas, Enterobacter, Bacillus, Pantoea, Pseudarthrobacter and Delftia, were selected for further analyses. These bacteria were identified using 16S rRNA gene sequencing.Antimicrobial assays of these selected bacteria revealed that Pseudomonas taiwanensis has strong anti-Phytophthora activity. Volatiles produced by P. taiwanensis inhibited growth of P. parasitica more than 80%. Ethyl acetate extracts of S. alboniger MOSEL-RD3, P. taiwanensis MOSEL-RD23, E. hormaechei MOSEL-FLS1 and B. tequilensis MOSEL-FLS3 and D. lacustris MB322 also displayed high potency against P. parasitica. All these bacterial extracts showed strong inhibition against P. parasitica at different concentrations (4 µg/mL -400 µg/mL).Bacterial extracts showing higher bioactivity (>80% inhibition in vitro) were selected for detached-leaf assay against P. parasitica on tobacco. In detached-leaf assay, application of 1% ethyl acetate bacterial extract of MOSE L-RD3, MOSEL-RD23, MOSEL-FLS1, MOSEL-FLS3 and MB322 reduced lesion sizes and lesion frequencies caused by P. parasitica by 68 to 81%. Over all P. taiwenensis MOSEL-RD23 showed positive activities for all the assays. Analysing the potential of bacterial endophytes as biological control agents can potentially lead to the formulation of broad-spectrum biopesticides for sustainable production of crops.

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“…1). Extensive studies have revealed that the Na(+)/K(+)-ATPase, with its highly specific ligands (CTSs), is capable of producing profound anticancer effects, including significant inhibition of proliferation, induction of apoptosis and cell cycle arrest in a large panel of cancer cells (Daniel et al, 2003;Waheed et al, 2012;Zhitu et al, 2012). 4.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, this class of compounds has received much attention as regards their potential as antineoplastic agents. Furthermore, in vitro and in vivo epidemiological data suggested that plant-derived cardiac steroids mediated anticancer activities through a regulated multitude of cellular processes such as proliferation, apoptosis and cell cycle arrest in various types of cancer cell lines, including human MCF-7, MDA-MB2311, prostate, melanoma, hepatoma cell, pancreatic, lung, colon cancer cells (Felth et al, 2009) and leukemia cell lines (Jiang et al, 2010;Waheed et al, 2012;Zhitu et al, 2012). Moreover, systematic works of the past few years, have discovered that polyphenol compounds have been playing an important role in protecting nerve cells from oxidative damage.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis Inducer Capacity of Cardiotonic Steroids of <i>Urginea maritima</i> Extract on SH-SY5Y Neuroblastoma Cells, with Less Susceptibility among Neuron-Module Cells

Elghuol¹,

Khalil

Zain

et al. 2016

American Journal of Applied Sciences

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Natural products have played a significant role in conventional treatment and in the development of new drugs for a wide range of illnesses, including malignant disease. Urginea maritima is classified as an herb is endemic to the Mediterranean area. This herb was renowned in traditional ethnopharmacological applications. Notwithstanding, there is a lack of evidence pertaining to the characterization of the Urginea maritima constituents contained within a water-based extract and their bioactivity in human neurological disorders. Therefore, we sought to investigate the composition and to evaluate its antitumour capability against the human malignant neuroblastoma cells. Through the utilization of several in vitro techniques, the present work illustrates that cardiotonic steroids are the main constituents of Urginea maritima aqueous extract and grouping of polyphenolic compounds are present. This result was based on interpretation of the mass spectrum obtained via electrospray ionization timeof-flight tandem mass spectrometry. The current study finding confirms that the constituents of the extract trigger a series of toxic reactions. In terms of cell proliferation inhibition, malignant SH-SY5Y neuroblastoma cell proliferation was selectively inhibited in a time-dose dependent manner. As verified quantitatively using the MTS colorimetric assay, with less toxicity was observed toward neuron-model cells. Apoptosis was efficiently induced in SH-SY5Y neuroblastoma cells as evidenced by Fluorescent microscopy using PI staining and DNA-specific dye DAPI revealed nuclear fragmentation, which profounds the apoptosis mechanism. Our novel findings clearly emphasize that the principal constituents of this unusual natural product can exhibit dual effects on neuron cells. This preferential selectivity provides an interesting basis for widespread medical application and a promising therapeutic strategy against neurological diseases, specifically human malignant neuroblastoma disorders.

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A novel steroidal saponin glycoside from Fagonia indica induces cell-selective apoptosis or necrosis in cancer cells

Cited by 60 publications

References 31 publications

Recent advances in steroidal saponins biosynthesis and in vitro production

Recent advances in steroidal saponins biosynthesis and in vitro production

Bioactivity-driven high throughput screening of microbiomes of medicinal plants for discovering new biological control agents

Apoptosis Inducer Capacity of Cardiotonic Steroids of <i>Urginea maritima</i> Extract on SH-SY5Y Neuroblastoma Cells, with Less Susceptibility among Neuron-Module Cells

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