A novel staining method for detecting phytase activity

Polypyrrole/phytase amperometric biosensors for the determination of phytic acid in standard solutions

“…In addition, techniques such as nuclear magnetic resonance spectroscopy, high-performance liquid chromatography, and SDS-polyacrylamide gel electrophoresis [11,12] have been also used for the detection of phytic acid in different analytes. The use of biosensors based on the immobilization of PhyA into polymeric matrices appoints as an alternative for the detection of phytic acid.…”

Replica plate screening method for detecting phosphatase activity in basidiomycetes using 1-Napthyl Phosphate as a chromogenic substrate

“…Phosphate solubilizing microorganisms (PSM) include bacteria and fungi, which can grow on various phosphorus containing compounds (Rodriguez & Fraga 1999). Even though Cobalt chloride reacts with phosphates to form a complex in pink colour, the precise mechanism involved in the counterstaining is not clearly defined but the addition of molybdate/vandate solution changes the colour from pink to yellow and enhances the contrast (Bae et al 1999). Effect of incubation on phosphatase activity showed the activity to increase with the colony development.…”

Screening of Soil Bacteria for Phytase Activity

“…Preliminary identification of the isolated strains was carried out on the basis of morphological analysis using Gram staining, microscopic observations and culture characteristics. The screening method for phytase activity was performed by using PSM (Bae et al, 1999). The PKA medium was used for testing inorganic phosphate solubilisation ability (Kaur and Reddy, 2013).…”