A novel SPR based method for measuring diffusion coefficients: From small molecules to supramolecular aggregates

Zingale, Gabriele Antonio; Pandino, Irene; Distefano, Alessia; Tuccitto, Nunzio; Grasso, Giuseppe

doi:10.1016/j.biosx.2023.100306

Cited by 2 publications

(4 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, sensitivity and precision in determining particles size by DLS strongly decreases if the particles are near the detection limit of this analytical technique and/or if adsorption phenomena of insulin to the solid interface occurs (Dathe et al, 1990). On the other hand, D ‐SPR technique is independent of the spherical assumption and it is gives very reliable results for particles as small as 100 Da (Zingale et al, 2023).…”

Section: Resultsmentioning

confidence: 99%

“…The system mounts two distinct lasers at two different wavelengths, 670 and 750 nm, respectively. We used both wavelengths for our purpose, whereas the two channels could be used independently for parallel experiments, as no subtraction with references is needed in the SPR diffusion experiments (Zingale et al, 2023). The latter were run on a surface which has been previously functionalized with a BSA layer to avoid any interaction of insulin with the gold surface (Distefano et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

“…We have recently reported that SPR can measure D for a variety of small molecules (Zingale et al, 2023b). Experiments are performed with solute particles, here insulin, that are introduced into a capillary tube filled with a carrier fluid, here the buffer solution.…”

Section: Theoretical Backgroundmentioning

confidence: 99%

“…A way to obtain information on the oligomerization/aggregation state of a biomolecule is to measure its diffusion coefficient ( D ) value, which is strictly correlated with the hydrodynamic radius of the molecular entity, through the well‐known Stokes–Einstein equation (Parker & Lollar, 2021). Recently, we have managed to obtain the D value of small molecules in water by applying a novel surface plasmon resonance (SPR) based method, which has been proven to be highly sensitive, reliable and reproducible for any molecule that does not interact with the gold surface of the SPR sensor chip (Zingale, Pandino, et al, 2023). The latter condition is of vital importance, as the interaction of the analyte with the surface would affect the SPR diffusion response, hindering the fitting of the experimental curves to obtain a reliable value of D .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Detection of insulin oligomeric forms by a novel surface plasmon resonance‐diffusion coefficient based approach

Calcagno,

Perina,

Zingale

et al. 2024

Protein Science

Self Cite

View full text Add to dashboard Cite

Insulin is commonly used to treat diabetes and undergoes aggregation at the site of repeated injections in diabetic patients. Moreover, aggregation is also observed during its industrial production and transport and should be avoided to preserve its bioavailability to correctly adjust glucose levels in diabetic patients. However, monitoring the effect of various parameters (pH, protein concentration, metal ions, etc.) on the insulin aggregation and oligomerization state is very challenging. In this work, we have applied a novel Surface Plasmon Resonance (SPR)‐based experimental approach to insulin solutions at various experimental conditions, monitoring how its diffusion coefficient is affected by pH and the presence of metal ions (copper and zinc) with unprecedented sensitivity, precision, and reproducibility. The reported SPR method, hereby applied to a protein for the first time, besides giving insight into the insulin oligomerization and aggregation phenomena, proved to be very robust for determining the diffusion coefficient of any biomolecule. A theoretical background is given together with the software description, specially designed to fit the experimental data. This new way of applying SPR represents an innovation in the bio‐sensing field and expanding the potentiality of commonly used SPR instruments well over the canonical investigation of biomolecular interactions.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Theoretical Backgroundmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of insulin oligomeric forms by a novel surface plasmon resonance‐diffusion coefficient based approach

Calcagno,

Perina,

Zingale

et al. 2024

Protein Science

Self Cite

View full text Add to dashboard Cite

show abstract

The Use of Surface Plasmon Resonance to Study the Interactions of Proteins Involved in Conformational Diseases: Experimental Approaches for New Therapeutical Perspectives

Zingale

Distefano

Grasso

2023

CMC

View full text Add to dashboard Cite

In recent years, the scientific community has been trying to tackle different diseases by using unifying and holistic approaches based on the concept that it is possible to target apparently very different diseases under a comprehensive general scheme. In other words, various different diseases have been grouped together under the label of “conformational diseases”, because the triggering cause for each malady is the misfolding of a specific protein, whose dyshomeostasis and accumulation cause all the other downhill biomolecular events characteristic of each different disease. In a parallel manner, analytical techniques have developed to investigate protein misfolding and accumulation, so as to give a valid technical support to the investigation of conformational diseases. In this scenario, surface plasmon resonance (SPR) has widely contributed to study many different aspects correlated to conformational diseases, offering the advantages of real time investigations, use of small amounts of biological materials and possibility to mimic the cellular environments without recurring to the use of fluorescent tags. In this review, after a brief introduction about conformational diseases and the SPR technique, a thorough description of the various uses of SPR to investigate the biomolecular mechanisms involved in these diseases is given in order to provide the reader with an exhaustive list as well as a critical perspective of the use of SPR for such topic. The case of Alzheimer’s disease is discussed at a deeper level. We hope that this work will make the reader aware of all the possible SPR experimental approaches, which can be used to develop new possible therapeutic strategies to tackle conformational diseases.

show abstract

A novel SPR based method for measuring diffusion coefficients: From small molecules to supramolecular aggregates

Cited by 2 publications

References 55 publications

Detection of insulin oligomeric forms by a novel surface plasmon resonance‐diffusion coefficient based approach

Detection of insulin oligomeric forms by a novel surface plasmon resonance‐diffusion coefficient based approach

The Use of Surface Plasmon Resonance to Study the Interactions of Proteins Involved in Conformational Diseases: Experimental Approaches for New Therapeutical Perspectives

Contact Info

Product

Resources

About