A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress

Abstract

Water is essential for plant growth and development. Water deficiency leads to loss of yield and decreased crop quality. To understand water transport mechanisms in plants, we cloned and characterized a novel tonoplast intrinsic protein (TIP) gene from soybean with the highest similarity to TIP2-type from other plants, and thus designated GmTIP2;3. The protein sequence contains two conserved NPA motifs and six transmembrane domains. The expression analysis indicated that this gene was constitutively expressed in all detected tissues, with higher levels in the root, stem and pod, and the accumulation of GmTIP2;3 transcript showed a significant response to osmotic stresses, including 20% PEG6000 (polyethylene glycol) and 100 μM ABA (abscisic acid) treatments. The promoter-GUS (glucuronidase) activity analysis suggested that GmTIP2;3 was also expressed in the root, stem, and leaf, and preferentially expressed in the stele of root and stem, and the core promoter region was 1000 bp in length, located upstream of the ATG start codon. The GUS tissue and induced expression observations were consistent with the findings in soybean. In addition, subcellular localization showed that GmTIP2;3 was a plasma membrane-localized protein. Yeast heterologous expression revealed that GmTIP2;3 could improve tolerance to osmotic stress in yeast cells. Integrating these results, GmTIP2;3 might play an important role in response to osmotic stress in plants.

Comparisons of Chlorophyll Fluorescence and Physiological Characteristics of Wheat Seedlings Influenced by Iso-Osmotic Stresses from Polyethylene Glycol and Sodium Chloride

“…Recent study on wheat seedling also reported that NaCl stress stimulated proline accumulation dramatically, PEG induced accumulation of sugar metabolites (glucose, fructose) and glycine betaine, while polyamines' synthetic pathway was also altered by various osmolytes [26]. Furthermore, under salt stress, turgor would be maintained by transporters such as Na + /H + antiporter, H + -pyrophosphatase, high efficiency potassium transport [5], and aquaporin, which is specific transporter for water [6].…”

Leaf Eh and pH: A Novel Indicator of Plant Stress. Spatial, Temporal and Genotypic Variability in Rice (Oryza sativa L.)

“…It would be interesting in further studies to assess the impact of various kinds of biotic and abiotic stresses on leaf Eh and pH and relate these indicators to stress intensity. Overproduction of reactive oxygen species (ROS) in plants is induced by various environmental perturbations like drought, heat, high light intensity, salinity, osmotic stress, chilling, herbicides, heavy metals, pathogens, wounding, ozone (O 3 ) and atmospheric pollutants [33][34][35]. It can be expected that these ROS affect Eh and pH at the plant level.…”

Assessment of Stress Tolerance, Productivity, and Forage Quality in T1 Transgenic Alfalfa Co-overexpressing ZxNHX and ZxVP1-1 from Zygophyllum xanthoxylum

“…In recent years, the development of molecular biology and transgenic engineering provides tools for creation of new cultivars with enhanced stress tolerance (Hasegawa et al, 2000; Herrera-Estrella, 2000; Yang et al, 2010; Tang et al, 2015; Zhang et al, 2016). Numerous studies have demonstrated that (co-)overexpression tonoplast NHX or/and H + -PPase genes is one of the most effective strategies to create transgenic species with enhanced salt and drought tolerance through genetic engineering technology (e.g., Apse et al, 1999; Gaxiola et al, 2001, 2007; Zhao et al, 2006; Liu et al, 2010; Bhaskaran and Savithramma, 2011; Pasapula et al, 2011; Gouiaa et al, 2012; Bao et al, 2014; Bassil and Blumwald, 2014).…”