A novel solid-state fermentation system using polyurethane foam as inert carrier

Abstract

A novel solid-state fermentation method using polyurethane foam as inert carrier impregnated with a synthetic liquid medium was developed simulating the nutritional composition and culture conditions of solid-state fermentation on wheat bran. With this system, biomass, the important parameter involved in solid-state fermentation processes, can be measured directly. Some other superiorities of this system over conventional solid-state fermentation systems are discussed.

Impact of process parameters on l-glutaminase production by marine Vibrio costicola in solid state fermentation using polystyrene as an inert support

“…These results cmphasise the critical role of phosphates and magnesium in the enhanced secretion of glutaminase. Zhu et al [4] have achieved increased yields of nuclease P1 with polyurethane foam impregnated with a media that simulated the chemical composition of wheat bran. In the present study, the maximal yield of the target product was obtained with the use of a sea water media containing L-glutamine, maltose and KH2PO4.…”

“…The use of nutritionally inert materials as supports for solid state fermentation (SSF) facilitates accurate designing of media, monitoring of process parameters, scaling-up strategies and various engineering aspects, which are either impossible or difficult with conventional SSF using organic solid substrates such as wheat bran [4]. The inert materials, when impregnated with a suitable medium, will provide a homogeneous aerobic condition throughout the fermentor, will not contribute impurities to the fermentation product [5] and will facilitate maximal recovery of the leachate with low *Present address: Research Centre, Department of Zoology, S. D. College, Alleppey 688 003, India.…”

“…We have recently reported that polystyrene, a commercially available insulating and packaging material, could be used as an inert solid support for the production of Lglutaminase by a marine Vibrio costicola under SSF [6], while ion exchange resins [8], polyurethane foam [4,9] and computer cards [10] have been used as inert carriers for SSF with fungi. In this paper we deal with the design of a suitable medium and the optimisation of process parameters towards maximal glutaminase production by a marine V. costicola in SSF using polystyrene as an inert support.…”

A comparison of methods to determine tannin acyl hydrolase activity

“…The SSF process involved the use of polyurethane foam (PUF) (Expomex, Mexico) as support to absorb the liquid medium. PUF was washed using the technique described by Zhu et al (1994) and then pulverized in a plastic-mill. Glass columns (25mm x 180 mm) were packed with 10 g of inoculated PUF(2 x 10 7 spores g -1 of dry inert carrier)impregnated with the medium (1.94 mL of inoculated medium per gram of dry inert carrier).…”

Clavulanic Acid Production by Streptomyces clavuligerus using Solid State Fermentation on Polyurethane Foam

“…For the selection of inert materials, polystyrene, which is a commercially-available insulating and packaging material, were used as the inert solid support for the production of enzymes [8][9][10]; while ion exchange resins [11], polyurethane foam [7,12,13], and vermiculite [14,15] have also been used as inert carriers for SSF. However, production of CA by inert support absorption SSF has not been yet reported.…”