A Novel Small Compound That Promotes Nuclear Translocation of YB-1 Ameliorates Experimental Hepatic Fibrosis in Mice

Abstract

Transforming growth factor-␤ (TGF-␤

Expression of the Clustered NeuAcα2–3Galβ O-Glycan Determines the Cell Differentiation State of the Cells

“…Immunoprecipitation and Microsequencing-Five milligrams of KP-3L cell lysates were prepared, and immunoprecipitation was performed using MAb7 followed by Dynabeads rat anti-mouse IgM as previously described (18). Proteins bound to beads were resolved by 7.5% SDS-PAGE and then visualized by silver staining.…”

“…Proteins bound to beads were resolved by 7.5% SDS-PAGE and then visualized by silver staining. Desired bands were excised, in-gel-digested with trypsin, and solvent-extracted, and the resulting MS/MS spectra were searched using the MASCOT search engine (18).…”

Dermokine‐β impairs ERK signaling through direct binding to GRP78

“…Stealth RNAi Negative Control Medium GC Duplex was used as a control (Invitrogen, Carlsbad, CA). Normal human keratinocytes were transfected with 2 lg of siRNA using Lipofectamine 2000 (Invitrogen) as previously described [10]. Seventy-two hours later, cells were incubated with recombinant dermokine-b.…”

The p63 Protein Isoform ΔNp63α Modulates Y-box Binding Protein 1 in Its Subcellular Distribution and Regulation of Cell Survival and Motility Genes

“…Moreover, nuclear translocation of YB-1 was shown to occur in response to DNA-damaging agents, phosphorylation at Ser-102 by AKT kinase, UV irradiation, TGF␤ stimulation, virus infection, hypothermia, and pharmacological compounds (1,8,9). Under stress stimuli, YB-1 nuclear translocation requires a physical interaction with wild type p53 (10).…”