A novel single precursor-based biodegradable hydrogel with enhanced mechanical properties

Abstract

A mechanically tough biodegradable hydrogel is developed from a single precursor comprising poly(ethylene glycol) and oligo(trimethylene carbonate), where both the crosslink density and swelling properties of the polymer network are independently controlled through M c and hydrophilic-hydrophobic balance. These highly cost effective hydrogels are also biocompatible and can be degraded both hydrolytically and enzymatically.

Engineering the cell–material interface for controlling stem cell adhesion, migration, and differentiation

“…The hydrogel rigidity (compressive modulus) was measured using Instron 3342 Universal Testing System (Instron, Norwood, MA, USA) equipped with a Model 2519-104 force transducer [25]. Briefly, equilibrium-swollen hydrogels in PBS were subjected to a maximum force load set to 450 N at a crosshead speed of 10 mm/min.…”

Preparation and characterization of Poly(γ-glutamic acid) hydrogels as potential tissue engineering scaffolds

“…Cell viability was evaluated using the Live/Dead assay kit after 24 h of photo-encapsulation according to reported protocol [20] . The live and dead cells were observed under fluorescence microscope (OLYMPUS-LX71 Inverted microscope with U-RFL-T fluorescence lamp), from which the live cells can be seen with green fluorescence, and the dead cells with red fluorescence.…”

“…Condensation of -PGA was always followed by side reactions. Compared with the above crosslinking methods, photo-crosslinking [20,21] , which does not involve harsh conditions such as high energy irradiation or toxic chemicals, is much more cytocompatible and can achieve better spatiotemporal control over the polymerization process and form more homogenous network structure [22] .…”

In vivo engineering of bone tissues with hematopoietic functions and mixed chimerism

“…Details of the synthesis of precursors as well as formation of macroporous matrices and their mineralization are described elsewhere (38)(39)(40)(41). The nonmineralized and mineralized matrices were assembled to create a dual-compartment structure with the outer compartment mineralized and the inner compartment either nonmineralized or hollow, emulating the structure of long-bone tissue (SI Appendix, Figs.…”