A novel signal amplification strategy of an electrochemical aptasensor for kanamycin, based on thionine functionalized graphene and hierarchical nanoporous PtCu

Qin, Xiaoli; Yin, Yan Sheng; Yu, Huijing; Guo, Wenjuan; Pei, Meishan

doi:10.1016/j.bios.2015.10.050

Cited by 114 publications

(23 citation statements)

References 44 publications

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“…Poly(thionine) has been used in various electrochemical sensors due to its admirable electrochemical signal and good H 2 O 2 catalytic ability, and thionine can be polymerised into poly(thionine)-Au using HAuCl 4 as the oxidizing agent 37, 38 . According to our results, the composite possessed the following outstanding advantages: (1) poly(thionine) displayed strong current signal at −0.15 V and excellent H 2 O 2 catalytic ability; (2) AuNPs can immobilise the antibody and further improve the electron transfer ability; and (3) the fabrication of this composite only requires an easy one-step method.…”

Section: Resultsmentioning

confidence: 99%

Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1

Wang

Gao

2017

Sci Rep

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Poly(thionine)-Au, a novel multifunctional substrate with excellent redox signal, enzyme-like activity, and easy antibody immobilisation, was synthesised using HAuCl4 as the oxidising agent and thionine as the monomer. The prepared poly(thionine)-Au composite exhibited an admirable electrochemical redox signal at −0.15 V and excellent H2O2 catalytic ability. In addition, gold nanoparticles in this composite were found to directly immobilise antibodies and further improve conductivity. In addition, a label-free electrochemical immunosensor was developed using poly(thionine)-Au as the sensing substrate for ultrasensitive detection of cytokeratin antigen 21-1 (CYFRA 21-1), an immunoassay found in human serum. The prepared immunosensor showed a wide liner range from 100 ng mL−1 to 10 fg mL−1 and an ultralow detection limit of 4.6 fg mL−1 (the ratio of signal to noise (S/N) = 3). Additionally, this method was used to analyse human serum samples and yielded results consistency with those of ELISA, implying its potential application in clinical research. The poly(thionine)-Au composite can be easily extended to other polymer-based nanocomposites, which is significant for other electrochemical immunoassays.

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Section: Resultsmentioning

confidence: 99%

Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1

Wang

Gao

2017

Sci Rep

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“…[12,13] Although these methods have some advantages, each one suffers from certain drawbacks including time consumption and low resolution. [19][20][21][22] Aptamers provide significant advantages such as adaptability to different targets; suitability in screening; reproducibility for synthesis; and flexibility in labelling, immobilization, signalling, and regeneration in comparison to traditional molecular recognition elements in sensor platforms. [14] Here, we have constructed quantum dot (QDs) decorated μ-well surfaces for the fluorometric detection of cocaine abuse by detecting of both cocaine and BE.…”

Section: Introductionmentioning

confidence: 99%

“…Aptamer targets could vary from small organic molecules/biomolecules to whole organisms. [19][20][21][22] Aptamers provide significant advantages such as adaptability to different targets; suitability in screening; reproducibility for synthesis; and flexibility in labelling, immobilization, signalling, and regeneration in comparison to traditional molecular recognition elements in sensor platforms. [23] In cocaine detection studies, different strategies such as electrochemical and electro-chemiluminescence methods were designed via several labelling agents such as redox probes to enhance the signal that forms from cocaine's presence (riboflavin, ruthenium complexes, etc).…”

Section: Introductionmentioning

confidence: 99%

An aptamer folding‐based sensory platform decorated with nanoparticles for simple cocaine testing

Guler

Bozokalfa

Demir

et al. 2016

Drug Testing and Analysis

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The consumption of illicit drugs such as cannabis, cocaine, and amphetamines is still a major health and social problem, creating an abuse in adults especially. Novel techniques which estimate the drug of abuse are needed for the detection of newly revealed psychoactive drugs. Herein, we have constructed a combinatorial platform by using quantum dots (QDs) and gold nanoparticles (AuNPs) as well as a functional aptamer which selectively recognizes cocaine and its metabolite benzoylecgonine (BE). We have called it an aptamer folding-based sensory device (AFSD). For the fabrication of AFSD, QDs were initially immobilized onto the poly-L-lysine coated μ-well surfaces. Then, the AuNP-aptamer conjugates were bound to the QDs. The addition of cocaine or BE caused a change in the aptamer structure which induced the close interaction of AuNPs with the QDs. Hence, quenching of the fluorescence of QDs was observed depending on the analyte amount. The linearity of cocaine and BE was 1.0-10 nM and 1.0-25 μM, respectively. Moreover, the limits of detection for cocaine and BE were calculated as 0.138 nM and 1.66 μM. The selectivity was tested by using different interfering substances (methamphetamine, bovine serum albumin, codeine, and 3-acetamidophenol). To investigate the use of AFSD in artificial urine matrix, cocaine/BE spiked samples were applied. Also, confirmatory analyses by using high performance liquid chromatography were performed. It is shown that AFSD has a good potential for testing the cocaine abuse and can be easily adapted for detection of various addictive drugs by changing the aptamer according to desired analytes. Copyright © 2016 John Wiley & Sons, Ltd.

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“…Antibiotics have been widely used in animals to cure various diseases and as growth promoters1. However, antibiotics can accumulate in the human body through the food chain, which may cause a variety of side effects1 and has resulted in the appearance of “super bacteria” with antibiotic tolerance2.…”

mentioning

confidence: 99%

“…However, antibiotics can accumulate in the human body through the food chain, which may cause a variety of side effects1 and has resulted in the appearance of “super bacteria” with antibiotic tolerance2. Therefore, it is important to analyze the maximum residue limits (MRLs) of residual antibiotics in foodstuffs.…”

mentioning

confidence: 99%

Ultra-sensitive detection of kanamycin for food safety using a reduced graphene oxide-based fluorescent aptasensor

Jung

et al. 2017

Sci Rep

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Overuse of antibiotics has caused serious problems, such as appearance of super bacteria, whose accumulation in the human body through the food chain is a concern. Kanamycin is a common antibiotic used to treat diverse infections; however, residual kanamycin can cause many side effects in humans. Thus, development of an ultra-sensitive, precise, and simple detection system for residual kanamycin in food products is urgently needed for food safety. In this study, we identified kanamycin-binding aptamers via a new screening method, and truncated variants were analyzed for optimization of the minimal sequence required for target binding. We found various aptamers with high binding affinity from 34.7 to 669 nanomolar Kdapp values with good specificity against kanamycin. Furthermore, we developed a reduced graphene oxide (RGO)-based fluorescent aptasensor for kanamycin detection. In this system, kanamycin was detected at a concentration as low as 1 pM (582.6 fg/mL). In addition, this method could detect kanamycin accurately in kanamycin-spiked blood serum and milk samples. Consequently, this simple, rapid, and sensitive kanamycin detection system with newly structural and functional analysis aptamer exhibits outstanding detection compared to previous methods and provides a new possibility for point of care testing and food safety.

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A novel signal amplification strategy of an electrochemical aptasensor for kanamycin, based on thionine functionalized graphene and hierarchical nanoporous PtCu

Cited by 114 publications

References 44 publications

Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1

Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1

An aptamer folding‐based sensory platform decorated with nanoparticles for simple cocaine testing

Ultra-sensitive detection of kanamycin for food safety using a reduced graphene oxide-based fluorescent aptasensor

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