A novel SERS-based magnetic aptasensor for prostate specific antigen assay with high sensitivity

Kang, Yang; Hu, Yongjun; Dong, Ning; Zhu, Guichi; Zhu, Tingfeng; Jiang, Ningjing

doi:10.1016/j.bios.2017.02.048

Cited by 97 publications

(45 citation statements)

References 55 publications

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“…Several SERS platforms have been reported for early-stage prostate cancer diagnosis. Some of the more researched morphologies include MNP-AuNP (Figure 11a) [233] and MNP-AgNP assemblies [234]. SERS-based genetic assays have been reported to be four orders of magnitude more sensitive than polymerase chain reaction (PCR) assays [235].…”

Section: Sers Biosensorsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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Section: Sers Biosensorsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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“…The DL for PSA quantification was 0.1 ng mL −1 , which was well below the clinical threshold for prostate cancer. Recently, the SER‐based detection of PSA using aptamer conjugated core–shell magnetic NPs (MNPs)‐Au NP nanoassemblies has also been described in Figure . The DL for PSA recognition was calculated to be 5.0 pg mL −1 , thereby demonstrating the excellent sensitivity of the nanoprobe.…”

Section: Nanomaterial‐based Cancer Sensing Systemsmentioning

confidence: 99%

Section: Nanomaterial‐based Cancer Sensing Systemsmentioning

confidence: 99%

Advanced Functional Structure‐Based Sensing and Imaging Strategies for Cancer Detection: Possibilities, Opportunities, Challenges, and Prospects

Kumar

Kukkar

Hashemi

et al. 2019

Adv Funct Materials

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Cancer is the second most common cause of death in the world. The principal limitations thus far encountered in the clinical practice of probing cancer are diverse and include low sensitivity, time consumption, bulkiness, and cost. In this respect, nanomaterial (NM)-based sensing techniques are recognized as a superior alternative to efficiently resolve such limitations. A better understanding of NM-based sensing platforms is thus important so that these novel avenues can easily be explored for clinical applications. These platforms have the merits of high sensitivity, high specificity, rapid response, and easy-to-read signals. This review offers a comprehensive survey of NM-based advanced cancer-sensing techniques and will help the scientific community establish optimum sensing strategies based on an accurate assessment of the interactions between cancer biomarkers and NM-based platforms.but are not limited to, carcinoma, sarcoma, leukemia, lymphoma, non-Hodgkin lymphoma, myeloma, central nervous system cancer, lung and bronchus cancer, colon cancer, rectum cancer, prostate cancer, bladder cancer, kidney and renal pelvis cancer, endometrial cancer, pancreatic cancer, liver cancer, and thyroid cancers. [1] Moreover, the most commonly occurring cancers tend to differ by common criteria like sex and age. For example, men are at risk for prostate, lung, and colorectal cancer, women are at risk for breast, lung and colorectal cancer, and children are at risk for leukemia, brain tumors, and lymphoma. The severity of the cancer depends upon the development level or staging of the disease. [2] Likewise, the type of treatment required to treat the cancer is determined by the staging of the disease.The more severe condition of cancer is metastasis, i.e., the uncontrolled growth of cells that invade surrounding tissues. The metastasis condition of cancer can affect any organ of the body. [3] As per the WHO 2010 report on cancer, the lives of 30% patients could have been saved if the cancer was diagnosed earlier. The detection of cancer is not easy because the immune system does not necessarily consider cancerous cells as foreign bodies. However, a few changes (such as altered biomarker levels and the presence of cancerous cells in biological fluids) can be detected. [4] In general, cancer biomarkers are overexpressed proteins present in blood/serum and at the cancer cell surface. The accurate and efficient detection of these biomarkers can be useful for cancer detection. The major problem with biomarkers is their low level in the body during the earlier stages of cancer. Hence, an efficient sensor/device capable of detecting these molecules even at very low levels, if developed, could help the clinician efficiently diagnose cancer cells in the human body.The sensing techniques presently used by clinicians suffer from several limitations; specifically, they are time consuming, bulky, have low sensitivity, and are expensive. Nanomaterial (NM)-based techniques have become an important alternative to the laborious conventional...

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“…Some other studies used core-satellite nanostructures for enhancing Raman reporter molecules that were placed at the hot spots but not for determining the concentrations of molecules 30,31,33,34,48–50 . Many of the core-satellite nanostructures used for SERS detection of molecules were based on detection schemes that utilized either DNA aptamers and Raman reporter molecules 28,29,36,37,51–53 or certain kinds of chemical interactions 54–56 , but the availability of suitable aptamers and chemical reactions could limit the use of the nanostructures for the detection of other molecules. Some of the SERS substrates formed with core-satellite nanostructures did not have many SERS hot spots in the z-direction, and the density of the nanostructures in the xy-plane was not maximized 14,27,57 .…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional SERS Substrates Formed with Plasmonic Core-Satellite Nanostructures

Wu¹,

Li²,

Lin

et al. 2017

Sci Rep

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We demonstrate three-dimensional surface-enhanced Raman spectroscopy (SERS) substrates formed by accumulating plasmonic nanostructures that are synthesized using a DNA-assisted assembly method. We densely immobilize Au nanoparticles (AuNPs) on polymer beads to form core-satellite nanostructures for detecting molecules by SERS. The experimental parameters affecting the AuNP immobilization, including salt concentration and the number ratio of the AuNPs to the polymer beads, are tested to achieve a high density of the immobilized AuNPs. To create electromagnetic hot spots for sensitive SERS sensing, we add a Ag shell to the AuNPs to reduce the interparticle distance further, and we carefully adjust the thickness of the shell to optimize the SERS effects. In addition, to obtain sensitive and reproducible SERS results, instead of using the core-satellite nanostructures dispersed in solution directly, we prepare SERS substrates consisting of closely packed nanostructures by drying nanostructure-containing droplets on hydrophobic surfaces. The densely distributed small and well-controlled nanogaps on the accumulated nanostructures function as three-dimensional SERS hot spots. Our results show that the SERS spectra obtained using the substrates are much stronger and more reproducible than that obtained using the nanostructures dispersed in solution. Sensitive detection of melamine and sodium thiocyanate (NaSCN) are achieved using the SERS substrates.

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A novel SERS-based magnetic aptasensor for prostate specific antigen assay with high sensitivity

Cited by 97 publications

References 55 publications

Recent Progress in Optical Sensors for Biomedical Diagnostics

Recent Progress in Optical Sensors for Biomedical Diagnostics

Advanced Functional Structure‐Based Sensing and Imaging Strategies for Cancer Detection: Possibilities, Opportunities, Challenges, and Prospects

Three-Dimensional SERS Substrates Formed with Plasmonic Core-Satellite Nanostructures

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