A novel screening on the specific peptide by molecular simulation and development of the electrochemical immunosensor for aflatoxin B1 in grains

Liu, Bing; Peng, Jiaxuan; Wu, Qiuyue; Zhao, Yaoshuai; Shang, Hua; Wang, Shuo

doi:10.1016/j.foodchem.2021.131322

Cited by 29 publications

(7 citation statements)

References 25 publications

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“…Both of the peptides exhibited a binding strength to OTA with K D value in the micromolar range, i.e., 11 to 15.7 µM, which are similar with those obtained by combinational chemistry [203,204]. With the advantages of easy availability and low cost, however, specific peptides have been developed only towards OTA and AFB1 [205]. The application of specific peptides as recognition elements in determination of other mycotoxins are not available yet.…”

Section: Molecularly Imprinted Polymers (Mips)mentioning

confidence: 53%

“…Biosensors are portable bioanalytical devices that incorporate biological recognition elements for binding of target molecules and a signal transducer to convert the biorecognition event into a measurable signal [333]. Based on various bioinspired recognition elements, mycotoxin sensors can be categorized into immunosensors [334], aptasensors [335,336], peptide-based sensors [205], and MIPs-based sensors [337,338]. Compared with other analytical methods mentioned above, it is much easier to realize real-time monitoring of reaction changes dynamically using biosensors, with output of the results in digital formats.…”

Section: Biosensorsmentioning

confidence: 99%

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Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review

Wang

Zhang

Wang

et al. 2022

Toxins

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Mycotoxins are secondary metabolites produced by fungal species, which pose significant risk to humans and livestock. The mycotoxins which are produced from Aspergillus, Penicillium, and Fusarium are considered most important and therefore regulated in food- and feedstuffs. Analyses are predominantly performed by official laboratory methods in centralized labs by expert technicians. There is an urgent demand for new low-cost, easy-to-use, and portable analytical devices for rapid on-site determination. Most significant advances were realized in the field bioanalytical techniques based on molecular recognition. This review aims to discuss recent progress in the generation of native biomolecules and new bioinspired materials towards mycotoxins for the development of reliable bioreceptor-based analytical methods. After brief presentation of basic knowledge regarding characteristics of most important mycotoxins, the generation, benefits, and limitations of present and emerging biorecognition molecules, such as polyclonal (pAb), monoclonal (mAb), recombinant antibodies (rAb), aptamers, short peptides, and molecularly imprinted polymers (MIPs), are discussed. Hereinafter, the use of binders in different areas of application, including sample preparation, microplate- and tube-based assays, lateral flow devices, and biosensors, is highlighted. Special focus, on a global scale, is placed on commercial availability of single receptor molecules, test-kits, and biosensor platforms using multiplexed bead-based suspension assays and planar biochip arrays. Future outlook is given with special emphasis on new challenges, such as increasing use of rAb based on synthetic and naïve antibody libraries to renounce animal immunization, multiple-analyte test-kits and high-throughput multiplexing, and determination of masked mycotoxins, including stereoisomeric degradation products.

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Section: Molecularly Imprinted Polymers (Mips)mentioning

confidence: 53%

Section: Biosensorsmentioning

confidence: 99%

Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review

Wang

Zhang

Wang

et al. 2022

Toxins

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“…Furthermore, the raw material used, such as indium tin oxide, is expensive. Similarly, the AuNPs in the electrochemical immunosensor presented by Liu and co-workers [ 70 ] are expensive as well. Unlike the existing electrochemical sensor, the PAni-based aptasensor is inexpensive with a low LOD of 0.01 nM, high repeatability of up to four times, good stability within 5 days of storage at 4 °C, and a facile fabrication method.…”

Section: Resultsmentioning

confidence: 99%

Impedimetric Polyaniline-Based Aptasensor for Aflatoxin B1 Determination in Agricultural Products

Ong

Phang

et al. 2023

Foods

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An impedimetric aptasensor based on a polyaniline (PAni) support matrix is developed through the surface modification of a screen-printed carbon electrode (SPE) for aflatoxin B1 (AFB1) detection in foodstuffs and feedstuffs for food safety. The PAni is synthesized with the chemical oxidation method and characterized with potentiostat/galvanostat, FTIR, and UV–vis spectroscopy techniques. The stepwise fabrication procedure of the PAni-based aptasensor is characterized by means of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods. The impedimetric aptasensor is optimized using the EIS technique, and its feasibility of detecting AFB1 in real sample matrices is evaluated via a recovery study in spiked foodstuffs and feedstuffs, such as pistachio nuts, cinnamons, cloves, corn, and soybeans, with a good recovery percentage, ranging from 87.9% to 94.7%. The charge transfer resistance (RCT) at the aptasensor interface increases linearly with the AFB1 concentration in the range of 3 × 10−2 nM to 8 × 10−2 nM, with a regression coefficient (R2) value of 0.9991 and detection limit of 0.01 nM. The proposed aptasensor is highly selective towards AFB1 and partially selective to AFB2 and ochratoxin A (OTA) due to their similar structures that differ only at the carbon–carbon double bond located at C8 and C9 and the large molecule size of OTA.

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“…Romanazzo et al [ 155 ] devised an enzyme-linked-immunomagnetic-electrochemical (ELIME) test to detect DON, utilizing anti-DON Fab fragments with a sensor's functional range of 100–4500 ng/mL. Similarly, Liu et al [ 156 ] used molecular docking and amino acid mutation techniques to generate a peptide library targeting AFB 1 . The assays showed a 9.4 × 10^-4 μg/L LOD alongside a linear range between 0.01 μg/L and 20 μg/L for AFB 1 .…”

Section: Mycotoxin Detectionmentioning

confidence: 99%

A comprehensive review of mycotoxins: Toxicology, detection, and effective mitigation approaches

Khan,

Anwar,

Ghazali

2024

Heliyon

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A novel screening on the specific peptide by molecular simulation and development of the electrochemical immunosensor for aflatoxin B1 in grains

Cited by 29 publications

References 25 publications

Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review

Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review

Impedimetric Polyaniline-Based Aptasensor for Aflatoxin B1 Determination in Agricultural Products

A comprehensive review of mycotoxins: Toxicology, detection, and effective mitigation approaches

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