A novel sample preparation method to avoid influence of embedding medium during nano-indentation

Meng, Yonggang; Wang, Siqun; Cai, Zhiyong; Young, Timothy M.; Du, Guanben; Li, Yanjun

doi:10.1007/s00339-012-7123-z

Cited by 51 publications

(21 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is well-known that the procedure of sample preparation before embedding as well as the embedding medium itself can modify cell wall properties [15,16] but not that much for LR-White or other acrylic-like resin [17,18], especially with the reduction in time at each step of the embedding process used in the present study. The authors thus consider that stiffness values are not significantly affected by the embedding resin in the present study.…”

Section: Sample Preparation Prior Atomic Force Microscopy (Afm) Peak mentioning

confidence: 93%

Investigation of the Mechanical Properties of Flax Cell Walls during Plant Development: The Relation between Performance and Cell Wall Structure

Goudenhooft

Siniscalco

Arnould

et al. 2018

Fibers

View full text Add to dashboard Cite

Abstract:The development of flax (Linum usitatissimum L.) fibers was studied to obtain better insight on the progression of their high mechanical performances during plant growth. Fibers at two steps of plant development were studied, namely the end of the fast growth period and at plant maturity, each time at three plant heights. The indentation modulus of the fiber cell wall was characterized by atomic force microscopy (AFM) using peak-force quantitative nano-mechanical property mapping (PF-QNM). Changes in the cell wall modulus with the cell wall thickening were highlighted. For growing plants, fibers from top and middle heights show a loose inner Gn layer with a lower indentation modulus than mature fibers, which exhibit thickened homogeneous cell walls made only of a G layer. The influence of these changes in the fiber cell wall on the mechanical performances of extracted elementary fibers was also emphasized by tensile tests. In addition, Raman spectra were recorded on samples from both growing and mature plants. The results suggest that, for the fiber cell wall, the cellulose contribution increases with fiber maturity, leading to a greater cell wall modulus of flax fibers.

show abstract

Section: Sample Preparation Prior Atomic Force Microscopy (Afm) Peak mentioning

confidence: 93%

Investigation of the Mechanical Properties of Flax Cell Walls during Plant Development: The Relation between Performance and Cell Wall Structure

Goudenhooft

Siniscalco

Arnould

et al. 2018

Fibers

View full text Add to dashboard Cite

show abstract

“…The dimension of the samples for nanoindentation was 8 × 8 × 1 mm 3 , and the slicing was done according to Meng et al (2013) . The small pieces were sealed with a polymer fi lm in FoodSaver vacuum sealing.…”

Section: Methodsmentioning

confidence: 99%

Quasi-static and dynamic nanoindentation to determine the influence of thermal treatment on the mechanical properties of bamboo cell walls

Li¹,

Yin²,

Huang³

et al. 2014

Holzforschung

Self Cite

View full text Add to dashboard Cite

Bamboo was thermally treated at 180 ° C and 200 ° C, and the micromechanical properties of its cell walls were investigated by means of quasi-static and dynamic nanoindentation experiments. With increasing treatment temperatures, the average dry density and mass of the bamboo decreased, whereas the already reduced elastic modulus at 180 ° C of the fiber cell walls did not change, but the hardness showed increasing tendencies. Dynamic nanoindentation revealed reduced storage modulus r ( ) E′ and loss modulus r ( ) E′′ for the thermotreated bamboo cell walls compared with the untreated bamboo fibers in all frequency regions. Moreover, r r , , E E ′ ′′ and loss tangent (tan δ ) of treated bamboo decreased with increasing treatment temperature.

show abstract

“…According to the method in Meng et al (2013), the samples were tested without embedded epoxy resin to avoid resin penetration. Each sample was mounted on a metal holder designed for ultramicrotomy to obtain a smooth surface with a diamond knife.…”

Section: Nanoindentation Measurementmentioning

confidence: 99%

“…Recent developments in these measurement techniques have further extended the usefulness of indentation tests at the cell-wall level of biological tissues (Wimmer et al 1997). During the past decade, a large number of useful engineering properties of biomaterials, including wood (Yu et al 2011;Meng et al 2013;Wang et al 2014), bamboo (Li et al 2015), crop stalks , and others, have been identified by means of nanoindentation.…”

Section: Introductionmentioning

confidence: 99%

Contributions of Basic Chemical Components to the Mechanical Behavior of Wood Fiber Cell Walls as Evaluated by Nanoindentation

Wang¹,

Li²,

Deng³

et al. 2016

BioResources

Self Cite

View full text Add to dashboard Cite

Selective chemical extraction was applied to gradually remove classes of chemical components from wood cell walls. Nanoindentation was performed on the control and treated wood cell walls to evaluate the contributions of the chemical components to the cell walls by measuring the elastic modulus, hardness, and creep compliance. Burger's model was applied to simulate the process of nanoindentation and to gain insight into the response of visco-elastic properties to the chemical components. Wood extractives showed limited effects on the cell-wall mechanics; however, the removal of hemicelluloses and lignin resulted in reductions of 11.7% and 28.4%, respectively, in the elastic modulus and 14.8% and 30.4%, respectively, in the hardness. The extraction of hemicelluloses and lignin reduced the resistance of wood cell walls to creep. Furthermore, the extracted parameters from Burger's modeling indicated that cellulose exhibited the greatest influence on the mechanical properties of wood cell wall, while hemicelluloses exhibited the greatest contribution to cell-wall viscosity, and lignin contributed extensively to cell-wall elasticity.

show abstract

A novel sample preparation method to avoid influence of embedding medium during nano-indentation

Cited by 51 publications

References 28 publications

Investigation of the Mechanical Properties of Flax Cell Walls during Plant Development: The Relation between Performance and Cell Wall Structure

Investigation of the Mechanical Properties of Flax Cell Walls during Plant Development: The Relation between Performance and Cell Wall Structure

Quasi-static and dynamic nanoindentation to determine the influence of thermal treatment on the mechanical properties of bamboo cell walls

Contributions of Basic Chemical Components to the Mechanical Behavior of Wood Fiber Cell Walls as Evaluated by Nanoindentation

Contact Info

Product

Resources

About