A novel role for Trichoderma secondary metabolites in the interactions with plants

Vinale, Francesco; Sivasithamparam, Krishnapillai; Ghisalberti, Emilio L.; Marra, Roberta; Barbetti, Martin J.; Li, H.; Woo, Sheridan L.; Lorito, Matteo

doi:10.1016/j.pmpp.2008.05.005

Cited by 464 publications

(361 citation statements)

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“…Flavonoids and phenolics acids are the principal groups of secondary metabolites and bioactive compounds in plants known for their role in plant resistance. Recently, the involvement of secondary metabolites in modulating plant health and resistance has been studied thoroughly (Vinale et al 2008(Vinale et al , 2009. The results are also in conformity with the study of other researchers who also showed promising effect of microbes on enhancement of phenolics and flavnoids (Gupta and Pandey 2015;Singh et al 2015).…”

Section: Resultssupporting

confidence: 83%

“…Likewise, the compounds mycosubtilin, rhizocticins, antifungal phosphonate oligopeptides and cyclic lipopeptide produced by different strains of B. subtilis enhanced the organism's antagonistic and plant growth promotion activities (Leclère et al 2005;Borisova et al 2010;Malfanova et al 2012). Vinale et al (2008) reported Trichoderma species to be well known producers of compounds that substantially affect the metabolism of the plant. In a study conducted by Verma et al (2014) T. harzanium culture filtrate at the dose of 3.3 % v/v was able to induce maximum biomass accumulation in embryogenic cell suspensions of latex free Papaver somniferum.…”

Section: Resultsmentioning

confidence: 99%

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Microbial secondary metabolites ameliorate growth, in planta contents and lignification in Withania somnifera (L.) Dunal

Singh

Gupta

Srivastava

et al. 2016

Physiol Mol Biol Plants

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In the present investigation, metabolites of Streptomyces sp. MTN14 and Trichoderma harzianum ThU significantly enhanced biomass yield (3.58 and 3.48 fold respectively) in comparison to the control plants. The secondary metabolites treatments also showed significant augmentation (0.75-2.25 fold) in withanolide A, a plant secondary metabolite. Lignin deposition, total phenolic and flavonoid content in W. somnifera were maximally induced in treatment having T. harzianum metabolites. Also, Trichoderma and Streptomyces metabolites were found much better in invoking in planta contents and antioxidants compared with their live culture treatments. Therefore, identification of new molecular effectors from metabolites of efficient microbes may be used as biopesticide and biofertilizer for commercial production of W. somnifera globally.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Microbial secondary metabolites ameliorate growth, in planta contents and lignification in Withania somnifera (L.) Dunal

Singh

Gupta

Srivastava

et al. 2016

Physiol Mol Biol Plants

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“…One such beneficial micro-organism is Trichoderma, a common soil fungus that has been shown to be a credible alternative to pesticides in the control of plant disease (Harman et al, 2004;Verma et al, 2007). In addition to disease control, certain strains also exhibit P-G-P activities (Bae et al, 2009;Contreras-Cornejo et al, 2009;Harman et al, 2004;Ortíz-Castro et al, 2009;Verma et al, 2007;Vinale et al, 2008Vinale et al, , 2009). These dual attributes make Trichoderma species attractive as both biocontrol agents and plant growth stimulants.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to their biocontrol properties, certain strains have been shown to enhance crop productivity by stimulating plant growth (Contreras-Cornejo et al, 2009;Harman et al, 2004;Ortíz-Castro et al, 2009;Vinale et al, 2008Vinale et al, , 2009. While genetic modification of Trichoderma strains by constitutive overexpression of chitinase, b-glucanase and proteinase genes has allowed the development of strains with improved biocontrol capabilities (Flores et al, 1997;Baek et al, 1999;Limó n et al, 1999;Viterbo et al, 2001;Djonović et al, 2007), less attention has been paid to enhancing the plantgrowth-promoting (P-G-P) activities of these fungi via genetic modification, and the impact that any such modification might have on their saprotrophic competence and fitness as biocontrol agents.…”

Section: Introductionmentioning

confidence: 99%

Saprotrophic competitiveness and biocontrol fitness of a genetically modified strain of the plant-growth-promoting fungus Trichoderma hamatum GD12

et al. 2012

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Trichoderma species are ubiquitous soil fungi that hold enormous potential for the development of credible alternatives to agrochemicals and synthetic fertilizers in sustainable crop production. In this paper, we show that substantial improvements in plant productivity can be met by genetic modification of a plant-growth-promoting and biocontrol strain of Trichoderma hamatum, but that these improvements are obtained in the absence of disease pressure only. Using a quantitative monoclonal antibody-based ELISA, we show that an N-acetyl-b-D-glucosaminidase-deficient mutant of T. hamatum, generated by insertional mutagenesis of the corresponding gene, has impaired saprotrophic competitiveness during antagonistic interactions with Rhizoctonia solani in soil. Furthermore, its fitness as a biocontrol agent of the pre-emergence damping-off pathogen Sclerotinia sclerotiorum is significantly reduced, and its ability to promote plant growth is constrained by the presence of both pathogens. This work shows that while gains in T. hamatummediated plant-growth-promotion can be met through genetic manipulation of a single beneficial trait, such a modification has negative impacts on other aspects of its biology and ecology that contribute to its success as a saprotrophic competitor and antagonist of soil-borne pathogens. The work has important implications for fungal morphogenesis, demonstrating a clear link between hyphal architecture and secretory potential. Furthermore, it highlights the need for a holistic approach to the development of genetically modified Trichoderma strains for use as crop stimulants and biocontrol agents in plant agriculture. INTRODUCTIONTrichoderma species are ubiquitous soil saprotrophs that have attracted sustained scientific interest as biological control agents of plant disease. In addition to their biocontrol properties, certain strains have been shown to enhance crop productivity by stimulating plant growth (Contreras-Cornejo et al., 2009;Harman et al., 2004;Ortíz-Castro et al., 2009;Vinale et al., 2008Vinale et al., , 2009. While genetic modification of Trichoderma strains by constitutive overexpression of chitinase, b-glucanase and proteinase genes has allowed the development of strains with improved biocontrol capabilities (Flores et al., 1997;Baek et al., 1999;Limó n et al., 1999;Viterbo et al., 2001;Djonović et al., 2007), less attention has been paid to enhancing the plantgrowth-promoting (P-G-P) activities of these fungi via genetic modification, and the impact that any such modification might have on their saprotrophic competence and fitness as biocontrol agents.In a previous study, we showed that a naturally occurring strain of Trichoderma hamatum was able to promote plant growth in low pH, nutrient-poor peat soils (Thornton, 2008). These soils contain a significant pool of nitrogen sequestered in chitin of insect and fungal origin (Kerley & Read, 1998), and fungal N-acetyl-b-D-glucosaminidase has been shown to be a key chitinolytic enzyme releasing sequestered nitrogen for assimilation ...

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“…La antibiosis de Trichoderma es por producción de sustancias como tricodermina, tricodermol, tricotoxina, dermadina [19] [20]; mientras que Bacillus y Pseudomonas producen metabolitos antifúngicos como: L. C. Oliva Ortiz et al…”

Section: Introductionunclassified

Morphology and Biochemistry of Microbial Antagonists and Their Effect in the Growth of Garbanzo and Control of Fusariosis

Ortiz¹,

Alcaraz²,

Perez³

et al. 2016

OALib

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The chickpea (Cicer arietinum L.) is an important crop in the world. In this study, six strains of indigenous rhizosphere microorganisms (T442, T3241, 7A1, 751, HRG-050 y HRG-060) were characterized morphologically and biochemically through catalogs and API tests, and evaluated as growth promoters and biocontrol agents of F. oxysporum f. sp. ciceris (Foc), causal agent of Fusarium wilt of chickpea under laboratory and field conditions. Preliminary studies were performed in vitro, evaluating antagonism by PICR of Foc, in Petri dishes with PDA culture medium; the stimulation of germination was evaluated by seed inoculation with the same strains. In the field, chickpea seeds were inoculated with said antagonistic strains at a concentration of 1 × 10 8 ufc or conidia mL −1, establishing eight treatments: six based on the inoculation of seed with each antagonist strain, a chemical treatment (Benomyl) and a witness, in block design randomized complete, evaluating the protective effect and stimulatory in chickpea plants. The strains were characterized as Bacillus subtilis, Agrobacterium radiobacter, Pseudomonas sp., Trichoderma sp., and Bacillus megaterium, the latter with low percentage of identification. HRG-060 (Trichoderma sp.) and T442 (Bacillus subtilis) proved to be the best biocontrol agents in laboratory and field, as well as higher growth promoters, as significantly reduced growth of the pathogen and the incidence of the disease in the field, and increased the germination, greenness, biomass and yield of chickpea. The use of strains HRG-060 y T442 to control Fusarium wilt of chickpea may be possible in the conditions that have the central area of Sinaloa.

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A novel role for Trichoderma secondary metabolites in the interactions with plants

Cited by 464 publications

References 32 publications

Microbial secondary metabolites ameliorate growth, in planta contents and lignification in Withania somnifera (L.) Dunal

Microbial secondary metabolites ameliorate growth, in planta contents and lignification in Withania somnifera (L.) Dunal

Saprotrophic competitiveness and biocontrol fitness of a genetically modified strain of the plant-growth-promoting fungus Trichoderma hamatum GD12

Morphology and Biochemistry of Microbial Antagonists and Their Effect in the Growth of Garbanzo and Control of Fusariosis

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