A novel rice calmodulin-like gene, OsMSR2, enhances drought and salt tolerance and increases ABA sensitivity in Arabidopsis

Xu, Guoyun; Rocha, Paulo Alexandre Costa; Wang, Manling; Xu, Ming; Cui, Yanchun; Li, Luoye; Zhu, Yu-Xian; Xia, X. Y.

doi:10.1007/s00425-011-1386-z

Cited by 189 publications

(126 citation statements)

References 48 publications

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“…In Arabidopsis there are three additional genes associated with the rgsCaM-like clade (CML37, CML39, and CML41). Previous work shows that these Arabidopsis proteins, as well as orthologs in rice (Oryza sativa), are also regulated by abiotic stresses (Vanderbeld and Snedden, 2007;Xu et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis CML38, a Calcium Sensor That Localizes to Ribonucleoprotein Complexes under Hypoxia Stress

et al. 2015

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During waterlogging and the associated oxygen deprivation stress, plants respond by the induction of adaptive programs, including the redirected expression of gene networks toward the synthesis of core hypoxia-response proteins. Among these core response proteins in Arabidopsis (Arabidopsis thaliana) is the calcium sensor CML38, a protein related to regulator of gene silencing calmodulin-like proteins (rgsCaMs). CML38 transcripts are up-regulated more than 300-fold in roots within 6 h of hypoxia treatment. Transfer DNA insertional mutants of CML38 show an enhanced sensitivity to hypoxia stress, with lowered survival and more severe inhibition of root and shoot growth. By using yellow fluorescent protein (YFP) translational fusions, CML38 protein was found to be localized to cytosolic granule structures similar in morphology to hypoxia-induced stress granules. Immunoprecipitation of CML38 from the roots of hypoxia-challenged transgenic plants harboring CML38pro::CML38: YFP followed by liquid chromatography-tandem mass spectrometry analysis revealed the presence of protein targets associated with messenger RNA ribonucleoprotein (mRNP) complexes including stress granules, which are known to accumulate as messenger RNA storage and triage centers during hypoxia. This finding is further supported by the colocalization of CML38 with the mRNP stress granule marker RNA Binding Protein 47 (RBP47) upon cotransfection of Nicotiana benthamiana leaves. Ruthenium Red treatment results in the loss of CML38 signal in cytosolic granules, suggesting that calcium is necessary for stress granule association. These results confirm that CML38 is a core hypoxia response calcium sensor protein and suggest that it serves as a potential calcium signaling target within stress granules and other mRNPs that accumulate during flooding stress responses.

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Section: Discussionmentioning

confidence: 99%

Arabidopsis CML38, a Calcium Sensor That Localizes to Ribonucleoprotein Complexes under Hypoxia Stress

et al. 2015

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“…Interestingly, local adaptation to coastal, potentially saline impacted environments has been shown before, where a sodium transporter allele conferred elevated salinity tolerance (Baxter et al, 2010). Since calcium sensing is a crucial pathway for salt stress tolerance in Arabidopsis (Guan et al, 2013), and has shown to be important for salt and drought stress in rice (Oryza sativa) (Xu et al, 2011), natural allelic variation at the CaS gene locus might be one way in which coastal populations adapt their growth to a saline environment. As such, our findings provide an excellent starting point to study the links between calcium signaling-dependent root growth and adaptation to growth in saline environments.…”

Section: Discussionmentioning

confidence: 99%

A Scalable Open-Source Pipeline for Large-Scale Root Phenotyping of Arabidopsis

et al. 2014

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Large-scale phenotyping of multicellular organisms is one of the current challenges in biology. We present a comprehensive and scalable pipeline that allows for the efficient phenotyping of root growth traits on a large scale. This includes a highresolution, low-cost acquisition setup as well as the automated image processing software BRAT. We assess the performance of this pipeline in Arabidopsis thaliana under multiple growth conditions and show its utility by performing genome-wide association studies on 16 root growth traits quantified by BRAT each day during a 5-d time-course experiment. The most significantly associated genome region for root growth rate is a locus encoding a calcium sensing receptor. We find that loss of function and overexpression of this gene can significantly alter root growth in a growth condition dependent manner and that the minor natural allele of the Calcium Sensor Receptor locus is highly significantly enriched in populations in coastal areas, demonstrating the power of our approach to identify regulators of root growth that might have adaptive relevance.

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“…The expression of specific stress-responsive genes has been demonstrated to be calciumregulated, for example, in response to cold (Knight et al, 1996;Knight et al, 1997;Tä htiharju et al, 1997;Galon et al, 2010a). The protein intermediates brokering calcium signals to regulate gene expression have also, in some cases, been identified, for example calmodulins (CaMs) (Takahashi et al, 2011), CaM-like proteins (Chiasson et al, 2005;Magnan et al, 2008;Xu et al, 2011), calcium-dependent protein kinases (CPKs) (Boudsocq et al, 2010;Coca and Segundo, 2010), CIPK/CBLs (Albrecht et al, 2003;Weinl and Kudla, 2009), and even transcription factors, such as CaM-binding transcription activators (CAMTAs) (Galon et al, 2008;Doherty et al, 2009;Du et al, 2009;Galon et al, 2010b).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis Reveals Calcium Regulation of Specific Promoter Motifs inArabidopsis

et al. 2011

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Increases in intracellular calcium concentration ([Ca 2+ ] c ) mediate plant responses to stress by regulating the expression of genes encoding proteins that confer tolerance. Several plant stress genes have previously been shown to be calciumregulated, and in one case, a specific promoter motif Abscisic Acid Responsive-Element (ABRE) has been found to be regulated by calcium. A comprehensive survey of the Arabidopsis thaliana transcriptome for calcium-regulated promoter motifs was performed by measuring the expression of genes in Arabidopsis seedlings responding to three calcium elevations of different characteristics, using full genome microarray analysis. This work revealed a total of 269 genes upregulated by [Ca 2+ ] c in Arabidopsis. Bioinformatic analysis strongly indicated that at least four promoter motifs were [Ca 2+ ] c -regulated in planta. We confirmed this finding by expressing in plants chimeric gene constructs controlled exclusively by these cis-elements and by testing the necessity and sufficiency of calcium for their expression. Our data reveal that the C-Repeat/Drought-Responsive Element, Site II, and CAM box (along with the previously identified ABRE) promoter motifs are calcium-regulated. The identification of these promoter elements targeted by the second messenger intracellular calcium has implications for plant signaling in response to a variety of stimuli, including cold, drought, and biotic stress.

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A novel rice calmodulin-like gene, OsMSR2, enhances drought and salt tolerance and increases ABA sensitivity in Arabidopsis

Cited by 189 publications

References 48 publications

Arabidopsis CML38, a Calcium Sensor That Localizes to Ribonucleoprotein Complexes under Hypoxia Stress

Arabidopsis CML38, a Calcium Sensor That Localizes to Ribonucleoprotein Complexes under Hypoxia Stress

A Scalable Open-Source Pipeline for Large-Scale Root Phenotyping of Arabidopsis

Transcriptomic Analysis Reveals Calcium Regulation of Specific Promoter Motifs inArabidopsis

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